Emily Freund
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View article: Cross-competition shapes CD8+ T cell hierarchies and differentiation after RNA vaccination
Cross-competition shapes CD8+ T cell hierarchies and differentiation after RNA vaccination Open
Short Summary Immunodominance is a universal feature of adaptive immunity that constrains T cell expansion, clonal diversity and breadth resulting in a narrowly focused T cell response. While observed across diverse priming settings and va…
View article: A single MHCII neoepitope mRNA vaccine elicits CD4 T- and B- cell responses promoting endogenous CD8 anti-tumor immunity
A single MHCII neoepitope mRNA vaccine elicits CD4 T- and B- cell responses promoting endogenous CD8 anti-tumor immunity Open
Summary Recent progress in therapeutic cancer vaccines has shown promising clinical activity, especially when targeting MHC class I (MHCI) neoantigen-specific CD8 + T cell responses in post-surgical patients. To explore the role of CD4 + T…
View article: Current Analytical Strategies for mRNA-Based Therapeutics
Current Analytical Strategies for mRNA-Based Therapeutics Open
Recent advancements in mRNA technology, utilized in vaccines, immunotherapies, protein replacement therapies, and genome editing, have emerged as promising and increasingly viable treatments. The rapid, potent, and transient properties of …
View article: Towards designing improved cancer immunotherapy targets with a peptide-MHC-I presentation model, HLApollo
Towards designing improved cancer immunotherapy targets with a peptide-MHC-I presentation model, HLApollo Open
View article: 1036 Recurrent RNA-lipoplex vaccination is required to replenish tumor-infiltrating neoantigen-specific T cells
1036 Recurrent RNA-lipoplex vaccination is required to replenish tumor-infiltrating neoantigen-specific T cells Open
View article: HLApollo: Towards designing improved cancer immunotherapy targets with a superior peptide-MHC-I presentation model
HLApollo: Towards designing improved cancer immunotherapy targets with a superior peptide-MHC-I presentation model Open
Based on the success of cancer immunotherapy, personalized cancer vaccines have recently emerged as the vanguard of oncology treatment. Because antigen presentation on MHC class I (MHC-I) is key to the adaptive immune response to cancerous…
View article: Systematic perturbation screens decode regulators of inflammatory macrophage states and identify a role for<i>TNF</i>mRNA m6A modification
Systematic perturbation screens decode regulators of inflammatory macrophage states and identify a role for<i>TNF</i>mRNA m6A modification Open
Macrophages adopt dynamic cell states with distinct effector functions to maintain tissue homeostasis and respond to environmental challenges. During chronic inflammation, macrophage polarization is subverted towards sustained inflammatory…
View article: Comprehensive Impurity Profiling of mRNA: Evaluating Current Technologies and Advanced Analytical Techniques
Comprehensive Impurity Profiling of mRNA: Evaluating Current Technologies and Advanced Analytical Techniques Open
In vitro transcription (IVT) of mRNA is a versatile platform for a broad range of biotechnological applications. Its rapid, scalable, and cost-effective production makes it a compelling choice for the development of mRNA-based cancer thera…
View article: Nucleotide modifications enable rational design of TLR7-selective ligands by blocking RNase cleavage
Nucleotide modifications enable rational design of TLR7-selective ligands by blocking RNase cleavage Open
Toll-like receptors 7 (TLR7) and 8 (TLR8) each sense single-stranded RNA (ssRNA), but their activation results in different immune activation profiles. Attempts to selectively target either TLR7 or TLR8 have been hindered by their high deg…
View article: 1130 Design and characterization of novel TLR7-selective ligands with an automated screening platform
1130 Design and characterization of novel TLR7-selective ligands with an automated screening platform Open
Background Toll-like receptors (TLRs) are an integral part of innate immune cell activation and play an essential role in mounting effective antigen-specific adaptive immune responses. TLR7 and TLR8 are highly homologous TLR family members…
View article: HLApollo: Towards designing improved cancer immunotherapy targets with a superior peptide-MHC-I presentation model
HLApollo: Towards designing improved cancer immunotherapy targets with a superior peptide-MHC-I presentation model Open
Based on the success of cancer immunotherapy, personalized cancer vaccines have recently emerged as the vanguard of oncology treatment. Because antigen presentation on MHC class I (MHC-I) is key to the adaptive immune response to c…
View article: HLApollo: A superior transformer model for pan-allelic peptide-MHC-I presentation prediction, with diverse negative coverage, deconvolution and protein language features
HLApollo: A superior transformer model for pan-allelic peptide-MHC-I presentation prediction, with diverse negative coverage, deconvolution and protein language features Open
Antigen presentation on MHC class I (MHC-I) is key to the adaptive immune response to cancerous cells. Computational prediction of peptide presentation by MHC-I has enabled individualized cancer immunotherapies. Here, we introduce HLApollo…
View article: IL-1 and IL-1ra are key regulators of the inflammatory response to RNA vaccines
IL-1 and IL-1ra are key regulators of the inflammatory response to RNA vaccines Open
View article: IL-1β and IL-1ra are key regulators of the inflammatory response to RNA vaccines
IL-1β and IL-1ra are key regulators of the inflammatory response to RNA vaccines Open
Excessive systemic inflammation is characteristic to various acute conditions including sepsis, viral infections and immunotherapy-induced adverse events such as cytokine release syndrome (CRS). Recently, several clinical trials evaluating…
View article: Efficient gene knockout in primary human and murine myeloid cells by non-viral delivery of CRISPR-Cas9
Efficient gene knockout in primary human and murine myeloid cells by non-viral delivery of CRISPR-Cas9 Open
Myeloid cells play critical and diverse roles in mammalian physiology, including tissue development and repair, innate defense against pathogens, and generation of adaptive immunity. As cells that show prolonged recruitment to sites of inj…
View article: Unbiased Identification of trans Regulators of ADAR and A-to-I RNA Editing
Unbiased Identification of trans Regulators of ADAR and A-to-I RNA Editing Open
View article: Zinc Finger RNA-Binding Protein Zn72D Regulates ADAR-Mediated RNA Editing in Neurons
Zinc Finger RNA-Binding Protein Zn72D Regulates ADAR-Mediated RNA Editing in Neurons Open
View article: Unbiased identification of <i>trans</i> regulators of ADAR and A-to-I RNA editing
Unbiased identification of <i>trans</i> regulators of ADAR and A-to-I RNA editing Open
Adenosine-to-Inosine RNA editing is catalyzed by ADAR enzymes that deaminate adenosine to inosine. While many RNA editing sites are known, few trans regulators have been identified. We perform BioID followed by mass-spectrometry to identif…
View article: Zinc finger RNA binding protein Zn72D regulates ADAR-mediated RNA editing in neurons
Zinc finger RNA binding protein Zn72D regulates ADAR-mediated RNA editing in neurons Open
Adenosine-to-inosine RNA editing, catalyzed by ADAR enzymes, alters RNA sequences from those encoded by DNA. These editing events are dynamically regulated, but few trans regulators of ADARs are known in vivo . Here, we screen RNA binding …