Emma Zsófia Aletta Nagy
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View article: Amino acid exchangeability and surface accessibility underpin the effects of single substitutions
Amino acid exchangeability and surface accessibility underpin the effects of single substitutions Open
Deep mutational scans have measured the effects of many mutations on many different proteins. Here we use a collection of such scans to perform a statistical meta-analysis of the effects of single amino acid substitutions. Specifically, we…
View article: Fluorescent enzyme-coupled activity assay for phenylalanine ammonia-lyases
Fluorescent enzyme-coupled activity assay for phenylalanine ammonia-lyases Open
Phenylalanine ammonia-lyases (PALs) catalyse the non-oxidative deamination of l -phenylalanine to trans -cinnamic acid, while in the presence of high ammonia concentration the reverse reaction occurs. PALs have been intensively studied, ho…
View article: Robust Suppression of Lipopolysaccharide Deficiency in Acinetobacter baumannii by Growth in Minimal Medium
Robust Suppression of Lipopolysaccharide Deficiency in Acinetobacter baumannii by Growth in Minimal Medium Open
Gram-negative bacteria are defined by their asymmetric outer membrane that consists of phospholipids on the inner leaflet and lipopolysaccharide (LPS) in the outer leaflet. LPS is essential in all but a few Gram-negative species; the reaso…
View article: Exploring the substrate scope of ferulic acid decarboxylase (FDC1) from Saccharomyces cerevisiae
Exploring the substrate scope of ferulic acid decarboxylase (FDC1) from Saccharomyces cerevisiae Open
Ferulic acid decarboxylase from Saccharomyces cerevisiae ( Sc FDC1) was described to possess a novel, prenylated flavin mononucleotide cofactor (prFMN) providing the first enzymatic 1,3-dipolar cycloaddition mechanism. The high tolerance o…
View article: A cluster of residues in the lipopolysaccharide exporter that selects substrate variants for transport to the outer membrane
A cluster of residues in the lipopolysaccharide exporter that selects substrate variants for transport to the outer membrane Open
Summary Most Gram‐negative bacteria assemble lipopolysaccharides (LPS) on their surface to form a permeability barrier against many antimicrobials. LPS is synthesized at the inner membrane and then transported to the outer leaflet of the o…
View article: Tailored Mutants of Phenylalanine Ammonia‐Lyase from <i>Petroselinum crispum</i> for the Synthesis of Bulky <scp>l</scp>‐ and <scp>d</scp>‐Arylalanines
Tailored Mutants of Phenylalanine Ammonia‐Lyase from <i>Petroselinum crispum</i> for the Synthesis of Bulky <span>l</span>‐ and <span>d</span>‐Arylalanines Open
Tailored mutants of phenylalanine ammonia‐lyase from Petroselinum crispum ( Pc PAL) were created and tested in ammonia elimination from various sterically demanding, non‐natural analogues of phenylalanine and in ammonia addition reactions …