Emmanuelle Thinon
YOU?
Author Swipe
View article: Affinity-based protein profiling of the antiviral natural product nanchangmycin
Affinity-based protein profiling of the antiviral natural product nanchangmycin Open
An affinity-based probe of the natural product nanchangmycin revealed a new mechanism by which this compound inhibits Zika virus infection.
View article: Affinity-based protein profiling of the antiviral natural product Nanchangmycin
Affinity-based protein profiling of the antiviral natural product Nanchangmycin Open
Nanchangmycin is a natural product with broad-spectrum activity against various organisms, exhibiting antibiotic, antiviral, anticancer, and antifibrotic effects. Nanchangmycin belongs to the family of polyether ionophores and is proposed …
View article: Optimal Stapling of a Helical Peptide‐Foldamer Hybrid Using a <i>C</i>‐Terminal 4‐Mercaptoproline Enhances Protein Surface Recognition and Cellular Activity
Optimal Stapling of a Helical Peptide‐Foldamer Hybrid Using a <i>C</i>‐Terminal 4‐Mercaptoproline Enhances Protein Surface Recognition and Cellular Activity Open
Structural analysis of a co‐crystal of a helically‐folded peptide‐foldamer hybrid in complex with hDM2 E3 ubiquitin ligase, revealed a unique orientation for the C ‐terminal proline with the pyrrolidine ring pointing backwards in the seque…
View article: Cell-Permeable Peptide Inhibitors of the p53-hDM2 Interaction via Foldamer Helix Mimicry and Bis-Thioether Stapling
Cell-Permeable Peptide Inhibitors of the p53-hDM2 Interaction via Foldamer Helix Mimicry and Bis-Thioether Stapling Open
Combining helical foldamers with α-peptides can produce α-helix mimetics with a reduced peptide character and enhanced resistance to proteolysis. Previously, we engineered a hybrid peptide-oligourea sequence replicating the N-terminal α-he…
View article: BFSP1 C-terminal domains released by post-translational processing events can alter significantly the calcium regulation of AQP0 water permeability
BFSP1 C-terminal domains released by post-translational processing events can alter significantly the calcium regulation of AQP0 water permeability Open
BFSP1 (beaded filament structural protein 1, filensin) is a cytoskeletal protein expressed in the eye lens. It binds AQP0 in vitro and its C-terminal sequences have been suggested to regulate the water channel activity of AQP0. A myristoyl…
View article: Selective Enrichment and Direct Analysis of Protein S-Palmitoylation Sites
Selective Enrichment and Direct Analysis of Protein S-Palmitoylation Sites Open
S-Fatty-acylation is the covalent attachment of long chain fatty acids, predominately palmitate (C16:0, S-palmitoylation), to cysteine (Cys) residues via a thioester linkage on proteins. This post-translational and reversible lipid modific…
View article: Mass‐Tag Labeling Using Acyl‐PEG Exchange for the Determination of Endogenous Protein S‐Fatty Acylation
Mass‐Tag Labeling Using Acyl‐PEG Exchange for the Determination of Endogenous Protein S‐Fatty Acylation Open
The covalent coupling of fatty acids to proteins provides an important mechanism of regulation in cells. In eukaryotes, cysteine fatty acylation (S‐fatty acylation) has been shown to be critical for protein function in a variety of cellula…
View article: Open Source High Content Analysis Utilizing Automated Fluorescence Lifetime Imaging Microscopy
Open Source High Content Analysis Utilizing Automated Fluorescence Lifetime Imaging Microscopy Open
We present an open source high content analysis instrument utilizing automated fluorescence lifetime imaging (FLIM) for assaying protein interactions using Förster resonance energy transfer (FRET) based readouts of fixed or live cells in m…
View article: Open Source High Content Analysis Utilizing Automated Fluorescence Lifetime Imaging Microscopy
Open Source High Content Analysis Utilizing Automated Fluorescence Lifetime Imaging Microscopy Open
We present an open source high content analysis instrument utilizing automated fluorescence lifetime imaging (FLIM) for assaying protein interactions using Förster resonance energy transfer (FRET) based readouts of fixed or live cells in m…
View article: Bioorthogonal Chemical Reporters for Monitoring Unsaturated Fatty‐Acylated Proteins
Bioorthogonal Chemical Reporters for Monitoring Unsaturated Fatty‐Acylated Proteins Open
Dietary unsaturated fatty acids, such as oleic acid, have been shown to be covalently incorporated into a small subset of proteins, but the generality and diversity of this protein modification has not been studied. We synthesized unsatura…
View article: <i>N</i>-Myristoyltransferase Inhibition Induces ER-Stress, Cell Cycle Arrest, and Apoptosis in Cancer Cells
<i>N</i>-Myristoyltransferase Inhibition Induces ER-Stress, Cell Cycle Arrest, and Apoptosis in Cancer Cells Open
N-Myristoyltransferase (NMT) covalently attaches a C14 fatty acid to the N-terminal glycine of proteins and has been proposed as a therapeutic target in cancer. We have recently shown that selective NMT inhibition leads to dose-responsive …
View article: Mass-tag labeling reveals site-specific and endogenous levels of protein S-fatty acylation
Mass-tag labeling reveals site-specific and endogenous levels of protein S-fatty acylation Open
Significance Cysteine fatty acylation (S-fatty acylation) regulates the stability, trafficking, and activity of proteins in eukaryotes. Current methods to study S-fatty acylation rely on the metabolic incorporation of fatty acid analogs or…
View article: Chemical reporters for exploring protein acylation
Chemical reporters for exploring protein acylation Open
Proteins are acylated by a variety of metabolites that regulates many important cellular pathways in all kingdoms of life. Acyl groups in cells can vary in structure from the smallest unit, acetate, to modified long-chain fatty acids, all …