Enrico Gratton
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View article: Highly multiplexed spectral FLIM via physics informed data analysis
Highly multiplexed spectral FLIM via physics informed data analysis Open
Spectral fluorescence lifetime imaging (S-FLIM) allows for the simultaneous deconvolution of signal from multiple fluorophore species by leveraging both spectral and lifetime information. However, existing analyses still face multiple diff…
View article: Fluorescence Lifetime Imaging Detects Long-Lifetime Signal Associated with Reduced Pyocyanin at the Surface of <i>Pseudomonas aeruginosa</i> Biofilms and in Cross-Feeding Conditions
Fluorescence Lifetime Imaging Detects Long-Lifetime Signal Associated with Reduced Pyocyanin at the Surface of <i>Pseudomonas aeruginosa</i> Biofilms and in Cross-Feeding Conditions Open
Understanding bacterial physiology in real-world environments requires noninvasive approaches and is a challenging yet necessary endeavor to effectively treat infectious disease. Bacteria evolve strategies to tolerate chemical gradients as…
View article: Pair correlation microscopy of intracellular molecular transport
Pair correlation microscopy of intracellular molecular transport Open
Pair correlation microscopy is a unique approach to fluorescence correlation spectroscopy that can track the long-range diffusive route of a population of fluorescent molecules in live cells with respect to intracellular architecture. This…
View article: Exploiting the detector distance information in image scanning microscopy by phasor-based SPLIT-ISM
Exploiting the detector distance information in image scanning microscopy by phasor-based SPLIT-ISM Open
Confocal microscopy is an important bio-imaging technique that increases the resolution using a spatial pinhole to block out-of-focus light. In theory, the maximum resolution and optical sectioning are obtained when the detection pinhole i…
View article: Micronuclear collapse from oxidative damage
Micronuclear collapse from oxidative damage Open
Chromosome-containing micronuclei are a hallmark of aggressive cancers. Micronuclei frequently undergo irreversible collapse, exposing their enclosed chromatin to the cytosol. Micronuclear rupture catalyzes chromosomal rearrangements, epig…
View article: Organelle phenotyping and multi‐dimensional microscopy identify C1q as a novel regulator of microglial function
Organelle phenotyping and multi‐dimensional microscopy identify C1q as a novel regulator of microglial function Open
Microglia, the immune cells of the central nervous system, are dynamic and heterogenous cells. While single cell RNA sequencing has become the conventional methodology for evaluating microglial state, transcriptomics do not provide insight…
View article: Abstract 4293: Chromosomal instability causes epigenetic aberrations in cancer
Abstract 4293: Chromosomal instability causes epigenetic aberrations in cancer Open
Chromosomal instability and changes in the epigenome are defining features in most metastatic cancers. Our research links chromosomal missegregation, their containment in micronuclei, and subsequent rupture of the micronuclear envelope to …
View article: Live-cell biosensors based on the fluorescence lifetime of environment-sensing dyes
Live-cell biosensors based on the fluorescence lifetime of environment-sensing dyes Open
In this work, we examine the use of environment-sensitive fluorescent dyes in fluorescence lifetime imaging microscopy (FLIM) biosensors. We screened merocyanine dyes to find an optimal combination of environment-induced lifetime changes, …
View article: Organelle phenotyping and multi-dimensional microscopy identify C1q as a novel regulator of microglial function
Organelle phenotyping and multi-dimensional microscopy identify C1q as a novel regulator of microglial function Open
Microglia, the immune cells of the central nervous system (CNS), are incredibly dynamic and heterogenous cells. While single cell RNA sequencing has become the conventional methodology for evaluating microglial state, transcriptomics do no…
View article: Comparison of MSD analysis from single particle tracking with MSD from images. Getting the best of both worlds
Comparison of MSD analysis from single particle tracking with MSD from images. Getting the best of both worlds Open
Fluorescence microscopy can provide valuable information about cell interior dynamics. Particularly, mean squared displacement (MSD) analysis is widely used to characterize proteins and sub-cellular structures’ mobility providing the laws …
View article: Building Fluorescence Lifetime Maps Photon-by-Photon by Leveraging Spatial Correlations
Building Fluorescence Lifetime Maps Photon-by-Photon by Leveraging Spatial Correlations Open
Fluorescence lifetime imaging microscopy (FLIM) has become a standard tool in the quantitative characterization of subcellular environments. However, quantitative FLIM analyses face several challenges. First, spatial correlations between p…
View article: Measuring protein-membrane interaction through radial fluorescence correlation in 2 dimensions
Measuring protein-membrane interaction through radial fluorescence correlation in 2 dimensions Open
The cell membrane has a fundamental role in the cell life cycle but there’s still much to be learned about its heterogeneous structure, regulation, and protein interaction. Additionally, the protein-membrane interaction is often overlooked…
View article: Epigenetic dysregulation from chromosomal transit in micronuclei
Epigenetic dysregulation from chromosomal transit in micronuclei Open
View article: Fluorescence lifetime: Beating the IRF and interpulse window
Fluorescence lifetime: Beating the IRF and interpulse window Open
View article: Building Fluorescence Lifetime Maps Photon-by-photon by Leveraging Spatial Correlations
Building Fluorescence Lifetime Maps Photon-by-photon by Leveraging Spatial Correlations Open
Fluorescence lifetime imaging microscopy (FLIM) has become a standard tool in the quantitative analysis of sub-cellular environments. However, quantitative FLIM analyses face several challenges. First, spatial correlations between pixels a…
View article: The role of FXR and TGR5 in reversing and preventing progression of Western diet–induced hepatic steatosis, inflammation, and fibrosis in mice
The role of FXR and TGR5 in reversing and preventing progression of Western diet–induced hepatic steatosis, inflammation, and fibrosis in mice Open
Nonalcoholic steatohepatitis (NASH) is the most common chronic liver disease in the US, partly due to the increasing incidence of metabolic syndrome, obesity, and type 2 diabetes. The roles of bile acids and their receptors, such as the nu…
View article: Phasor Analysis of Fluorescence Lifetime Enables Quantitative Multiplexed Molecular Imaging of Three Probes
Phasor Analysis of Fluorescence Lifetime Enables Quantitative Multiplexed Molecular Imaging of Three Probes Open
The excited-state lifetime is an intrinsic property of fluorescent molecules that can be leveraged for multiplexed imaging. An advantage of fluorescence lifetime-based multiplexing is that signals from multiple probes can be gathered simul…
View article: Fluorescence Lifetime: Beating the IRF and interpulse window
Fluorescence Lifetime: Beating the IRF and interpulse window Open
Fluorescence lifetime imaging (FLIM) has been essential in capturing spatial distributions of chemical species across cellular environments employing pulsed illumination confocal setups. However, quantitative interpretation of lifetime dat…
View article: Elucidating the Oligomerization and Cellular Interactions of a Trimer Derived from Aβ through Fluorescence and Mass Spectrometric Studies
Elucidating the Oligomerization and Cellular Interactions of a Trimer Derived from Aβ through Fluorescence and Mass Spectrometric Studies Open
Aβ oligomers play a central role in the neurodegeneration observed with Alzheimer's disease. Our laboratory has developed covalently stabilized trimers derived from residues 17-36 of Aβ as model systems for studying Aβ oligomers. In the cu…
View article: Author Correction: In vivo pair correlation microscopy reveals dengue virus capsid protein nucleocytoplasmic bidirectional movement in mammalian infected cells
Author Correction: In vivo pair correlation microscopy reveals dengue virus capsid protein nucleocytoplasmic bidirectional movement in mammalian infected cells Open
View article: Multiplexed bioluminescence microscopy via phasor analysis
Multiplexed bioluminescence microscopy via phasor analysis Open
View article: Radial pair correlation of molecular brightness fluctuations maps protein diffusion as a function of oligomeric state within live-cell nuclear architecture
Radial pair correlation of molecular brightness fluctuations maps protein diffusion as a function of oligomeric state within live-cell nuclear architecture Open
Nuclear proteins can modulate their DNA binding activity and the exploration volume available during DNA target search by self-associating into higher-order oligomers. Directly tracking this process in the nucleoplasm of a living cell is, …
View article: In vivo macromolecular crowding is differentially modulated by aquaporin 0 in zebrafish lens: Insights from a nanoenvironment sensor and spectral imaging
In vivo macromolecular crowding is differentially modulated by aquaporin 0 in zebrafish lens: Insights from a nanoenvironment sensor and spectral imaging Open
Macromolecular crowding is crucial for cellular homeostasis. In vivo studies of macromolecular crowding and water dynamics are needed to understand their roles in cellular physiology and fate determination. Macromolecular crowding in the l…
View article: High Resolution Fluorescence Lifetime Maps from Minimal Photon Counts
High Resolution Fluorescence Lifetime Maps from Minimal Photon Counts Open
Fluorescence lifetime imaging microscopy (FLIM) may reveal subcellular spatial lifetime maps of key molecular species. Yet, such a quantitative picture of life necessarily demands high photon budgets at every pixel under the current analys…
View article: Live cell biosensors based on the fluorescence lifetime of environment-sensing dyes
Live cell biosensors based on the fluorescence lifetime of environment-sensing dyes Open
Most biosensors used with fluorescence lifetime imaging (FLIM) have been based on fluorescence resonance energy transfer (FRET). Here we examined the capabilities of FLIM biosensors based on environment-sensing dyes. We screened merocyanin…
View article: High resolution fluorescence lifetime maps from minimal photon counts
High resolution fluorescence lifetime maps from minimal photon counts Open
View article: Epigenetic dysregulation from chromosomal transit in micronuclei
Epigenetic dysregulation from chromosomal transit in micronuclei Open
Chromosomal instability (CIN) and epigenetic alterations are characteristics of advanced and metastatic cancers [1-4], yet whether they are mechanistically linked is unknown. Here we show that missegregation of mitotic chromosomes, their s…
View article: Spatial transcriptomics using combinatorial fluorescence spectral and lifetime encoding, imaging and analysis
Spatial transcriptomics using combinatorial fluorescence spectral and lifetime encoding, imaging and analysis Open
View article: Fluorescence lifetime microscopy unveils the supramolecular organization of liposomal Doxorubicin
Fluorescence lifetime microscopy unveils the supramolecular organization of liposomal Doxorubicin Open
The supramolecular organization of Doxorubicin (DOX) within the standard Doxoves® liposomal formulation (DOX®) is investigated using visible light and phasor approach to fluorescence lifetime imaging (phasor-FLIM).
View article: Visualizing the mode of action and supramolecular assembly of teixobactin analogues in <i>Bacillus subtilis</i>
Visualizing the mode of action and supramolecular assembly of teixobactin analogues in <i>Bacillus subtilis</i> Open
FRET microscopy experiments demonstrate supramolecular assembly of teixobactin molecules on Bacillus subtilis , providing further evidence that teixobactin is a supramolecular antibiotic.