Eric A. Johnson
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View article: Unwrapping success: A scoping review of exam wrappers and metacognitive skill development in undergraduate nursing education
Unwrapping success: A scoping review of exam wrappers and metacognitive skill development in undergraduate nursing education Open
Exam wrappers are promising tools for developing metacognitive skills in nursing students. Embedding them early in curricula, using digital platforms and supporting feedback literacy may improve uptake and sustainability. Future studies sh…
View article: A Unified Framework for Anomaly Detection and Root Cause Analysis in Microservice Systems
A Unified Framework for Anomaly Detection and Root Cause Analysis in Microservice Systems Open
Modern software applications increasingly rely on microservice architectures for scalability, flexibility, and rapid deployment. However, this architectural paradigm introduces new complexities in monitoring system behavior, identifying an…
View article: Botulinum neurotoxin X lacks potency in mice and in human neurons
Botulinum neurotoxin X lacks potency in mice and in human neurons Open
Botulinum neurotoxins (BoNTs) are a class of toxins produced by Clostridium botulinum ( C. botulinum ) and other species of Clostridia . BoNT/X is a putative novel botulinum neurotoxin identified through genome sequencing and capable of SN…
View article: Analyzing the Role of Reactive Oxygen Species in Male-Female Interactions in Arabidopsis thaliana.
Analyzing the Role of Reactive Oxygen Species in Male-Female Interactions in Arabidopsis thaliana. Open
Fertilization, both in plants and animals, is at its core, a study of cell to cell communication. With respect to plants, the male gametophyte, the pollen tube, elongates within the female organ called the pistil, transporting in its cytop…
View article: A Novel Approach to Locating Community Clinics to Promote Health Care Accessibility and Reduce Health Disparities in Baltimore, Maryland
A Novel Approach to Locating Community Clinics to Promote Health Care Accessibility and Reduce Health Disparities in Baltimore, Maryland Open
Baltimore, Maryland’s entrenched racial residential segregation renders the city’s world-class medical facilities and services inaccessible to many Black residents living in its most divested neighborhoods. Arguing the need for post-pandem…
View article: Reduction in Long-term Mortality After Sleeve Gastrectomy and Gastric Bypass Compared to Nonsurgical Patients With Severe Obesity
Reduction in Long-term Mortality After Sleeve Gastrectomy and Gastric Bypass Compared to Nonsurgical Patients With Severe Obesity Open
Objective: To separately compare the long-term risk of mortality among bariatric surgical patients undergoing either Roux-en-Y gastric bypass (RYGB) or sleeve gastrectomy (SG) to large, matched, population-based cohorts of patients with se…
View article: Posttranslational Regulation of Botulinum Neurotoxin Production in Clostridium botulinum Hall A- <i>hyper</i>
Posttranslational Regulation of Botulinum Neurotoxin Production in Clostridium botulinum Hall A- <i>hyper</i> Open
Botulinum neurotoxin (BoNT) is a public health and bioterrorism concern as well as an important and widely used pharmaceutical, yet the regulation of its synthesis by BoNT-producing clostridia is not well understood. This paper highlights …
View article: Irreversible Inhibition of BoNT/A Protease: Unique Warhead Reactivity and Function Contingent upon a Bifunctional Approach
Irreversible Inhibition of BoNT/A Protease: Unique Warhead Reactivity and Function Contingent upon a Bifunctional Approach Open
We describe a comprehensive screening campaign of warheads, linked to a hydroxamate chelating anchor, for the modification of Cys165 within the BoNT/A protease. Engaging thorough enzyme kinetics, we detail a remarkable proximity-driven cov…
View article: Irreversible inhibition of BoNT/A protease: proximity-driven reactivity contingent upon a bifunctional approach
Irreversible inhibition of BoNT/A protease: proximity-driven reactivity contingent upon a bifunctional approach Open
A proximity-driven covalent bond with intrinsically less reactive warheads has been made possible by using a metal-chelating anchor for directed targeted covalent modification of Cys165 within the BoNT/A protease.
View article: Identification of 3-hydroxy-1,2-dimethylpyridine-4(1<i>H</i>)-thione as a metal-binding motif for the inhibition of botulinum neurotoxin A
Identification of 3-hydroxy-1,2-dimethylpyridine-4(1<i>H</i>)-thione as a metal-binding motif for the inhibition of botulinum neurotoxin A Open
The 3,4-HOPTO pharmacophore was identified as an inhibitor of botulinum neurotoxin A light chain. Structure-activity relationships were explored, resulting in a compound that protects SNAP-25 cleavage in motor neurons.
View article: Catch and Anchor Approach To Combat Both Toxicity and Longevity of Botulinum Toxin A
Catch and Anchor Approach To Combat Both Toxicity and Longevity of Botulinum Toxin A Open
Botulinum neurotoxins have remarkable persistence (∼weeks to months in cells), outlasting the small-molecule inhibitors designed to target them. To address this disconnect, inhibitors bearing two pharmacophores-a zinc binding group and a C…
View article: A Novel High-Potency Tetanus Vaccine
A Novel High-Potency Tetanus Vaccine Open
Chemical inactivation is a clinically effective mechanism to detoxify protein toxins to produce vaccines against microbial infections and to serve as a platform for production of conjugate polysaccharide vaccines. This method is widely use…
View article: Cryptic Patterns of Speciation in Cryptic Primates: Microendemic Mouse Lemurs and the Multispecies Coalescent
Cryptic Patterns of Speciation in Cryptic Primates: Microendemic Mouse Lemurs and the Multispecies Coalescent Open
Mouse lemurs (Microcebus) are a radiation of morphologically cryptic primates distributed throughout Madagascar for which the number of recognized species has exploded in the past two decades. This taxonomic revision has prompted understan…
View article: Measuring problem prescription opioid use among patients receiving long-term opioid analgesic treatment: development and evaluation of an algorithm for use in EHR and claims data
Measuring problem prescription opioid use among patients receiving long-term opioid analgesic treatment: development and evaluation of an algorithm for use in EHR and claims data Open
OBJECTIVE: Opioid surveillance in response to the opioid epidemic will benefit from scalable, automated algorithms for identifying patients with clinically documented signs of problem prescription opioid use. Existing algorithms lack accur…
View article: Critical Analysis of Neuronal Cell and the Mouse Bioassay for Detection of Botulinum Neurotoxins
Critical Analysis of Neuronal Cell and the Mouse Bioassay for Detection of Botulinum Neurotoxins Open
Botulinum Neurotoxins (BoNTs) are a large protein family that includes the most potent neurotoxins known to humankind. BoNTs delivered locally in humans at low doses are widely used pharmaceuticals. Reliable and quantitative detection of B…
View article: Botulinum neurotoxins A, B, C, E, and F preferentially enter cultured human motor neurons compared to other cultured human neuronal populations
Botulinum neurotoxins A, B, C, E, and F preferentially enter cultured human motor neurons compared to other cultured human neuronal populations Open
Human‐induced pluripotent stem cell (hi PSC )‐derived neurons can be exquisitely sensitive to botulinum neurotoxins (Bo NT s), exceeding sensitivity of the traditionally used mouse bioassay. In this report, four defined hi PSC ‐derived neu…
View article: Isolation and Characterization of the Novel Botulinum Neurotoxin A Subtype 6
Isolation and Characterization of the Novel Botulinum Neurotoxin A Subtype 6 Open
Botulinum neurotoxins (BoNTs) have proved to be an effective treatment for a large number of neuropathic conditions. BoNTs comprise a large family of zinc metalloproteases, but BoNT/A1 is used nearly exclusively for pharmaceutical purposes…
View article: Association Between Bariatric Surgery and Macrovascular Disease Outcomes in Patients With Type 2 Diabetes and Severe Obesity
Association Between Bariatric Surgery and Macrovascular Disease Outcomes in Patients With Type 2 Diabetes and Severe Obesity Open
In this observational study of patients with type 2 diabetes and severe obesity who underwent surgery, compared with those who did not undergo surgery, bariatric surgery was associated with a lower risk of macrovascular outcomes. The findi…
View article: Botulinum Neurotoxin Detection Methods for Public Health Response and Surveillance
Botulinum Neurotoxin Detection Methods for Public Health Response and Surveillance Open
Botulism outbreak due to consumption of food contaminated with botulinum neurotoxins (BoNTs) is a public health emergency. The threat of bioterrorism through deliberate distribution in food sources and/or aerosolization of BoNTs raises glo…
View article: The Light Chain Defines the Duration of Action of Botulinum Toxin Serotype A Subtypes
The Light Chain Defines the Duration of Action of Botulinum Toxin Serotype A Subtypes Open
Botulinum neurotoxin (BoNT) is the causative agent of botulism and a widely used pharmaceutical to treat a variety of neurological diseases. BoNTs are 150-kDa protein toxins organized into heavy chain (HC) and light chain (LC) domains link…
View article: A Study and Review of Effects of Botulinum Toxins on Mast Cell Dependent and Independent Pruritus
A Study and Review of Effects of Botulinum Toxins on Mast Cell Dependent and Independent Pruritus Open
Pruriceptive itch originates following activation of peripheral sensory nerve terminals when pruritogens come in contact with the skin. The ability of botulinum neurotoxins (BoNTs) to attenuate transmitter release from afferent terminals p…