Eric Betzig
YOU?
Author Swipe
View article: Mesoscale volumetric fluorescence imaging at nanoscale resolution by photochemical sectioning
Mesoscale volumetric fluorescence imaging at nanoscale resolution by photochemical sectioning Open
Optical nanoscopy of intact biological specimens has been transformed by recent advancements in hydrogel-based tissue clearing and expansion, enabling the imaging of cellular and subcellular structures with molecular contrast. However, exi…
View article: Fourier-based three-dimensional multistage transformer for aberration correction in multicellular specimens
Fourier-based three-dimensional multistage transformer for aberration correction in multicellular specimens Open
High-resolution tissue imaging is often compromised by sample-induced optical aberrations that degrade resolution and contrast. Although wavefront sensor-based adaptive optics (AO) can measure these aberrations, such hardware solutions are…
View article: A Multimodal Adaptive Optical Microscope For In Vivo Imaging from Molecules to Organisms
A Multimodal Adaptive Optical Microscope For In Vivo Imaging from Molecules to Organisms Open
Understanding biological systems requires observing features and processes across vast spatial and temporal scales, spanning nanometers to centimeters and milliseconds to days, often using multiple imaging modalities within complex native …
View article: Oblique line scan illumination enables expansive, accurate and sensitive single-protein measurements in solution and in living cells
Oblique line scan illumination enables expansive, accurate and sensitive single-protein measurements in solution and in living cells Open
An ideal tool for the study of cellular biology would enable the measure of molecular activity nondestructively within living cells. Single-molecule localization microscopy (SMLM) techniques, such as single-molecule tracking (SMT), enable …
View article: Software for lattice light-sheet imaging of FRET biosensors, illustrated with a new Rap1 biosensor
Software for lattice light-sheet imaging of FRET biosensors, illustrated with a new Rap1 biosensor Open
Lattice light-sheet microscopy (LLSM) is valuable for its combination of reduced photobleaching and outstanding spatiotemporal resolution in 3D. Using LLSM to image biosensors in living cells could provide unprecedented visualization of ra…
View article: Image processing tools for petabyte-scale light sheet microscopy data
Image processing tools for petabyte-scale light sheet microscopy data Open
Light sheet microscopy is a powerful technique for high-speed three-dimensional imaging of subcellular dynamics and large biological specimens. However, it often generates datasets ranging from hundreds of gigabytes to petabytes in size fo…
View article: Nanoscale volumetric fluorescence imaging via photochemical sectioning
Nanoscale volumetric fluorescence imaging via photochemical sectioning Open
Optical nanoscopy of intact biological specimens has been transformed by recent advancements in hydrogel-based tissue clearing and expansion, enabling the imaging of cellular and subcellular structures with molecular contrast. However, exi…
View article: Demo Live Cell Imaging Dataset for PetaKit5D
Demo Live Cell Imaging Dataset for PetaKit5D Open
Demo dataset for PetaKit5D (https://github.com/abcucberkeley/PetaKit5D). The dataset is for the large field of view live cell imaging of LLC-PK1 cells of nuclei and endoplasmic reticulum (ER). It contains two time points with two channels.…
View article: Oblique Line Scan Illumination Enables Expansive, Accurate and Sensitive Single Protein Measurements in Solution and in Living Cells
Oblique Line Scan Illumination Enables Expansive, Accurate and Sensitive Single Protein Measurements in Solution and in Living Cells Open
Single-molecule localization microscopy (SMLM) techniques, such as single-molecule tracking (SMT), enable in situ measurements in cells from which data-rich metrics can be extracted. SMT has been successfully applied to a variety of biolog…
View article: Demo Live Cell Imaging Dataset for LLSM5DTools
Demo Live Cell Imaging Dataset for LLSM5DTools Open
Demo dataset for LLSM5DTools (https://github.com/abcucberkeley/LLSM5DTools). The dataset is for the large field of view live cell imaging of LLC-PK1 cells of nuclei and endoplasmic reticulum (ER). It contains two time points with two chann…
View article: Image processing tools for petabyte-scale light sheet microscopy data
Image processing tools for petabyte-scale light sheet microscopy data Open
Light sheet microscopy is a powerful technique for high-speed 3D imaging of subcellular dynamics and large biological specimens. However, it often generates datasets ranging from hundreds of gigabytes to petabytes in size for a single expe…
View article: Oblique Line Scan Illumination Enables Expansive, Accurate and Sensitive Single Protein Measurements in Solution and in Living Cells
Oblique Line Scan Illumination Enables Expansive, Accurate and Sensitive Single Protein Measurements in Solution and in Living Cells Open
Single-molecule localization microscopy (SMLM) techniques, such as single-molecule tracking (SMT), enable in situ measurements in cells from which data-rich metrics can be extracted. SMT has been successfully applied to a variety of biolog…
View article: Seeing Beyond the Limits With Super-Resolution Microscopy
Seeing Beyond the Limits With Super-Resolution Microscopy Open
When I was young, I wanted to be an astronaut and I was a hard-core science fiction fan. I was always drawn to heroic characters that invented something new that resulted in an amazing breakthrough. When I started my scientific career, I d…
View article: Sensitivity optimization of a rhodopsin-based fluorescent voltage indicator
Sensitivity optimization of a rhodopsin-based fluorescent voltage indicator Open
The ability to optically image cellular transmembrane voltages at millisecond-timescale resolutions can offer unprecedented insight into the function of living brains in behaving animals. Here, we present a point mutation that increases th…
View article: Characterization, comparison, and optimization of lattice light sheets
Characterization, comparison, and optimization of lattice light sheets Open
Lattice light sheet microscopy excels at the noninvasive imaging of three-dimensional (3D) dynamic processes at high spatiotemporal resolution within cells and developing embryos. Recently, several papers have called into question the perf…
View article: Origin of wiring specificity in an olfactory map revealed by neuron type–specific, time-lapse imaging of dendrite targeting
Origin of wiring specificity in an olfactory map revealed by neuron type–specific, time-lapse imaging of dendrite targeting Open
How does wiring specificity of neural maps emerge during development? Formation of the adult Drosophila olfactory glomerular map begins with the patterning of projection neuron (PN) dendrites at the early pupal stage. To better understand …
View article: Author response: Origin of wiring specificity in an olfactory map revealed by neuron type–specific, time-lapse imaging of dendrite targeting
Author response: Origin of wiring specificity in an olfactory map revealed by neuron type–specific, time-lapse imaging of dendrite targeting Open
Full text Figures and data Side by side Abstract Editor's evaluation eLife digest Introduction Results Discussion Materials and methods Appendix 1 Data availability References Decision letter Author response Article and author information …
View article: Origin of wiring specificity in an olfactory map: dendrite targeting of projection neurons
Origin of wiring specificity in an olfactory map: dendrite targeting of projection neurons Open
How does wiring specificity of neural maps emerge during development? Formation of the adult Drosophila olfactory glomerular map begins with patterning of projection neuron (PN) dendrites at the early pupal stage. To better understand the …
View article: A proliferative to invasive switch is mediated by srGAP1 downregulation through the activation of TGF-β2 signaling
A proliferative to invasive switch is mediated by srGAP1 downregulation through the activation of TGF-β2 signaling Open
Many breast cancer (BC) patients suffer from complications of metastatic disease. To form metastases, cancer cells must become migratory and coordinate both invasive and proliferative programs at distant organs. Here, we identify srGAP1 as…
View article: Characterization, Comparison, and Optimization of Lattice Light Sheets
Characterization, Comparison, and Optimization of Lattice Light Sheets Open
Lattice light sheet microscopy excels at the non-invasive imaging of three-dimensional (3D) dynamic processes at high spatiotemporal resolution within cells and developing embryos. Recently, several papers have called into question the per…
View article: Superresolution microscopy reveals actomyosin dynamics in medioapical arrays
Superresolution microscopy reveals actomyosin dynamics in medioapical arrays Open
Superresolution microscopy produced images of individual actin and myosin filaments and their three-dimensional distribution in medioapical arrays of cells in developing embryos. Arrays are part of a modified cell cortex that condenses and…
View article: Cellular Bases of Olfactory Circuit Assembly Revealed by Systematic Time-lapse Imaging
Cellular Bases of Olfactory Circuit Assembly Revealed by Systematic Time-lapse Imaging Open
Neural circuit assembly features simultaneous targeting of numerous neuronal processes from constituent neuron types, yet the dynamics is poorly understood. Here, we use the Drosophila olfactory circuit to investigate dynamic cellular proc…
View article: u-track 3D: measuring and interrogating dense particle dynamics in three dimensions
u-track 3D: measuring and interrogating dense particle dynamics in three dimensions Open
Particle tracking is a ubiquitous task in the study of dynamic molecular and cellular processes through microscopy. Light-sheet microscopy has opened a path to acquiring complete cell volumes for investigation in 3-dimensions (3D). However…