Eric Biot
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<span>M2WISH</span>: An easy and efficient protocol for whole‐mount <span>mRNA</span><i>in situ</i> hybridization that allows <span>3D</span> cell resolution of gene expression in <i>Arabidopsis thaliana</i> Open
SUMMARY Gene expression analysis is essential for understanding the mechanisms involved in plant development. Here, we developed M2WISH, a protocol based on M icro W ave treatment for W holemount mRNA I n S itu H ybridization in Arabidopsi…
View article: Non-random spatial organization of telomeres varies during the cell cycle and requires LAP2 and BAF
Non-random spatial organization of telomeres varies during the cell cycle and requires LAP2 and BAF Open
Spatial genome organization within the nucleus influences major biological processes and is impacted by the configuration of linear chromosomes. Here, we applied 3D spatial statistics and modeling on high-resolution telomere and centromere…
M2WISH: an easy and efficient protocol for whole-mount mRNA <i>in situ</i> hybridization that allows 3D cell resolution of gene expression in <i>Arabidopsis thaliana</i> Open
Gene expression analysis is essential for understanding the mechanisms involved in plant development. Here, we developed M2WISH, a protocol based on M icro W ave treatment for W holemount mRNA In S itu H ybridization in Arabidopsis. By per…
Model-based reconstruction of whole organ growth dynamics reveals invariant patterns in leaf morphogenesis Open
Plant organ morphogenesis spans several orders of magnitude in time and space. Because of limitations in live-imaging, analysing whole organ growth from initiation to mature stages typically rely on static data sampled from different timep…
View article: Spatial modeling of telomere intra-nuclear distribution reveals non-random organization that varies during cell cycle and depends on LAP2 and BAF
Spatial modeling of telomere intra-nuclear distribution reveals non-random organization that varies during cell cycle and depends on LAP2 and BAF Open
Genome organization within the 3D nuclear volume influences major biological processes but is completely lost during mitosis, which represents a major challenge to maintain cellular identity and cell fate. To restore a functional G1 nucleu…
Recommendation: Model-based reconstruction of whole organ growth dynamics reveals invariant patterns in leaf morphogenesis — R2/PR10 Open
Plant organ morphogenesis spans several orders of magnitude in time and space. Because of limitations in live-imaging, analysing whole organ growth from initiation to mature stages typically rely on static data sampled from different timep…
Decision: Model-based reconstruction of whole organ growth dynamics reveals invariant patterns in leaf morphogenesis — R2/PR11 Open
Plant organ morphogenesis spans several orders of magnitude in time and space. Because of limitations in live-imaging, analysing whole organ growth from initiation to mature stages typically rely on static data sampled from different timep…
Decision: Model-based reconstruction of whole organ growth dynamics reveals invariant patterns in leaf morphogenesis — R1/PR8 Open
Plant organ morphogenesis spans several orders of magnitude in time and space. Because of limitations in live-imaging, analysing whole organ growth from initiation to mature stages typically rely on static data sampled from different timep…
New Flowering and Architecture Traits Mediated by Multiplex CRISPR-Cas9 Gene Editing in Hexaploid Camelina sativa Open
Adapting plants to sustainable cropping systems is a major challenge for facing climate change and promoting agroecological transition. Camelina sativa is an emerging oilseed crop species with climate-resilient properties that could be use…
Review: Model-based reconstruction of whole organ growth dynamics reveals invariant patterns in leaf morphogenesis — R1/PR6 Open
Plant organ morphogenesis spans several orders of magnitude in time and space. Because of limitations in live-imaging, analysing whole organ growth from initiation to mature stages typically rely on static data sampled from different timep…
Decision: Model-based reconstruction of whole organ growth dynamics reveals invariant patterns in leaf morphogenesis — R0/PR4 Open
Plant organ morphogenesis spans several orders of magnitude in time and space. Because of limitations in live-imaging, analysing whole organ growth from initiation to mature stages typically rely on static data sampled from different timep…
View article: A cell wall-associated gene network shapes leaf boundary domains
A cell wall-associated gene network shapes leaf boundary domains Open
Boundary domains delimit and organize organ growth throughout plant development almost relentlessly, building plant architecture and morphogenesis. Boundary domains display reduced growth and orchestrate development of adjacent tissues in …
Recommendation: Model-based reconstruction of whole organ growth dynamics reveals invariant patterns in leaf morphogenesis — R0/PR3 Open
Plant organ morphogenesis spans several orders of magnitude in time and space. Because of limitations in live-imaging, analysing whole organ growth from initiation to mature stages typically rely on static data sampled from different timep…
Review: Model-based reconstruction of whole organ growth dynamics reveals invariant patterns in leaf morphogenesis — R0/PR2 Open
Plant organ morphogenesis spans several orders of magnitude in time and space. Because of limitations in live-imaging, analysing whole organ growth from initiation to mature stages typically rely on static data sampled from different timep…
View article: A cell wall-associated gene network shapes leaf boundary domains
A cell wall-associated gene network shapes leaf boundary domains Open
Boundary domains delimit and organize organ growth throughout plant development almost relentlessly building plant architecture and morphogenesis. Boundary domains display reduced growth and orchestrate development of adjacent tissues in a…
Strategy and software for the statistical spatial analysis of 3D intracellular distributions Open
Summary The localization of proteins in specific domains or compartments in the 3D cellular space is essential for many fundamental processes in eukaryotic cells. Deciphering spatial organization principles within cells is a challenging ta…
Multiscale quantification of morphodynamics: MorphoLeaf, software for 2-D shape analysis Open
A major challenge in morphometrics is to analyse complex biological shapes formed by structures at different scales. Leaves exemplify this challenge as they combine differences in their overall shape with smaller shape variations at their …