Eric J. Rees
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View article: Role of novel polysaccharide layers in assembly of the exosporium, the outermost protein layer of the <i>Bacillus anthracis</i> spore
Role of novel polysaccharide layers in assembly of the exosporium, the outermost protein layer of the <i>Bacillus anthracis</i> spore Open
A fundamental question in cell biology is how cells assemble their outer layers. The bacterial endospore is a well‐established model for cell layer assembly. However, the assembly of the exosporium, a complex protein shell comprising the o…
View article: Improved RAD51 binders through motif shuffling based on the modularity of BRC repeats
Improved RAD51 binders through motif shuffling based on the modularity of BRC repeats Open
Significance Despite advances in directed evolution and computational design, engineering of functional proteins remains challenging. Here we demonstrate that rearrangement of modular repeats from BRCA2 can yield chimeras with improved int…
View article: Frame localisation optical projection tomography
Frame localisation optical projection tomography Open
We present a tomographic reconstruction algorithm (flOPT), which is applied to Optical Projection Tomography (OPT) images, that is robust to mechanical jitter and systematic angular and spatial drift. OPT relies on precise mechanical rotat…
View article: An open‐hardware sample mounting solution for inverted light‐sheet microscopes with large detection objective lenses
An open‐hardware sample mounting solution for inverted light‐sheet microscopes with large detection objective lenses Open
Summary Implementations of light‐sheet microscopes are often incompatible with standard methods of sample mounting. Light‐sheet microscopy uses orthogonal illumination and detection to create a thin sheet of light which does not illuminate…
View article: Improved RAD51 binders through motif shuffling based on the modularity of BRC repeats
Improved RAD51 binders through motif shuffling based on the modularity of BRC repeats Open
Exchanges of protein sequence modules support leaps in function unavailable through point mutations during evolution. Here we study the role of the two RAD51-interacting modules within the eight binding BRC repeats of BRCA2. We created 64 …
View article: Concepts for structured illumination microscopy with extended axial resolution through mirrored illumination
Concepts for structured illumination microscopy with extended axial resolution through mirrored illumination Open
Wide-field fluorescence microscopy, while much faster than confocal microscopy, suffers from a lack of optical sectioning and poor axial resolution. 3D structured illumination microscopy (SIM) has been demonstrated to provide optical secti…
View article: Concepts for structured illumination microscopy with extended axial resolution through mirrored illumination
Concepts for structured illumination microscopy with extended axial resolution through mirrored illumination Open
Wide-field fluorescence microscopy, while much faster than confocal microscopy, suffers from a lack of optical sectioning and poor axial resolution. 3D structured illumination microscopy (SIM) has been demonstrated to provide optical secti…
View article: Concepts for structured illumination microscopy with extended axial resolution through mirrored illumination
Concepts for structured illumination microscopy with extended axial resolution through mirrored illumination Open
Wide-field fluorescence microscopy, while much faster than confocal microscopy, suffers from a lack of optical sectioning and poor axial resolution. 3D structured illumination microscopy (SIM) has been demonstrated to provide optical secti…
View article: OptiJ: Open-source optical projection tomography of large organ samples
OptiJ: Open-source optical projection tomography of large organ samples Open
The three-dimensional imaging of mesoscopic samples with Optical Projection Tomography (OPT) has become a powerful tool for biomedical phenotyping studies. OPT uses visible light to visualize the 3D morphology of large transparent samples.…
View article: OptiJ: Open-source optical projection tomography of large organ samples
OptiJ: Open-source optical projection tomography of large organ samples Open
The three-dimensional imaging of mesoscopic samples with Optical Projection Tomography (OPT) has become a powerful tool for biomedical phenotyping studies. OPT uses visible light to visualize the 3D morphology of large transparent samples.…
View article: Novel cortex lytic enzymes in <i>Bacillus megaterium</i> QM B1551 spores
Novel cortex lytic enzymes in <i>Bacillus megaterium</i> QM B1551 spores Open
Present models for spore germination in Bacillus species include a requirement for either the SleB or CwlJ cortex lytic enzymes to efficiently depolymerise the spore cortex. Previous work has demonstrated that B. megaterium spores may diff…
View article: An Open-Hardware sample mounting solution for inverted light-sheet microscopes with large detection objective lenses
An Open-Hardware sample mounting solution for inverted light-sheet microscopes with large detection objective lenses Open
Implementations of light-sheet microscopes are often incompatible with standard methods of sample mounting. Light-sheet microscopy uses orthogonal illumination and detection to create a thin sheet of light which does not illuminate the sam…
View article: Improved methodology for performing the inverse Abel transform of flame images for color ratio pyrometry
Improved methodology for performing the inverse Abel transform of flame images for color ratio pyrometry Open
A new method is presented for performing the Abel inversion by fitting the line-of-sight projection of a predefined intensity distribution (FLiPPID) to the recorded 2D projections. The aim is to develop a methodology that is less prone to …
View article: Intra-mitochondrial proteostasis is directly coupled to alpha-synuclein and Amyloid β 1-42 pathology
Intra-mitochondrial proteostasis is directly coupled to alpha-synuclein and Amyloid β 1-42 pathology Open
Mitochondria have long been implicated in Parkinson’s disease (PD), however, it is not clear how mitochondrial impairment and alpha-synuclein pathology are coupled. We report here that intra-mitochondrial protein homeostasis plays a major …
View article: Structured illumination microscopy with extended axial resolution through mirrored illumination
Structured illumination microscopy with extended axial resolution through mirrored illumination Open
Wide-field fluorescence microscopy, while much faster than confocal microscopy, suffers from a lack of optical sectioning and poor axial resolution. 3D structured illumination microscopy (SIM) has been demonstrated to provide optical secti…
View article: An open-hardware sample mounting solution for light-sheet microscopes
An open-hardware sample mounting solution for light-sheet microscopes Open
Implementations of light-sheet microscopes are often incompatible with standard methods of sample mounting. Light-sheet microscopy uses orthogonal illumination and detection to create a thin sheet of light which does not illuminate the sam…
View article: Research data supporting: Proteins encoded by the gerP operon are localised to the inner coat in Bacillus cereus spores and are dependent on GerPA for assembly
Research data supporting: Proteins encoded by the gerP operon are localised to the inner coat in Bacillus cereus spores and are dependent on GerPA for assembly Open
Image data, image analysis software, and numerical results in support of a paper on protein location in B. cereus spores
View article: Research data supporting: Proteins Encoded by the gerP Operon Are Localized to the Inner Coat in Bacillus cereus Spores and Are Dependent on GerPA and SafA for Assembly
Research data supporting: Proteins Encoded by the gerP Operon Are Localized to the Inner Coat in Bacillus cereus Spores and Are Dependent on GerPA and SafA for Assembly Open
Image data and numerical results of image analysis. The numerical results are summarised in the excel file: GerP_results_Supporting_data, and pages within that file describe the contents of the folders of image data.
View article: Proteins Encoded by the <i>gerP</i> Operon Are Localized to the Inner Coat in Bacillus cereus Spores and Are Dependent on GerPA and SafA for Assembly
Proteins Encoded by the <i>gerP</i> Operon Are Localized to the Inner Coat in Bacillus cereus Spores and Are Dependent on GerPA and SafA for Assembly Open
The germination of Bacillus spores is triggered by certain amino acids and sugar molecules which permeate the outermost layers of the spore to interact with receptor complexes that reside in the inner membrane. Previous studies have shown …
View article: ELM: super-resolution analysis of wide-field images of fluorescent shell structures
ELM: super-resolution analysis of wide-field images of fluorescent shell structures Open
It is often necessary to precisely quantify the size of specimens in biological studies. When measuring feature size in fluorescence microscopy, significant biases can arise due to blurring of its edges if the feature is smaller than the d…
View article: Homographically generated light sheets for the microscopy of large specimens
Homographically generated light sheets for the microscopy of large specimens Open
We compare the performance of linear and nonlinear methods for aligning the excitation and detection planes throughout volumes of large specimens in digitally scanned light sheet microscopy. An effective nonlinear method involves the regis…
View article: Microbiome Structural and Functional Interactions across Host Dietary Niche Space
Microbiome Structural and Functional Interactions across Host Dietary Niche Space Open
Host-associated microbiomes are integral components of host health, but microbiome community structure varies among and within hosts. Reconciling community variability with the apparent dependence of hosts on community function, and charac…
View article: Generation of strength in a drying film: How fracture toughness depends on dispersion properties
Generation of strength in a drying film: How fracture toughness depends on dispersion properties Open
The fracture toughness of colloidal films is measured by characterizing cracks which form during directional drying. Images from a confocal microscope are processed to measure the crack width as a function of distance from the crack tip. A…
View article: Quantitative Affinity Determination by Fluorescence Anisotropy Measurements of Individual Nanoliter Droplets
Quantitative Affinity Determination by Fluorescence Anisotropy Measurements of Individual Nanoliter Droplets Open
Fluorescence anisotropy measurements of reagents compartmentalized into individual nanoliter droplets are shown to yield high-resolution binding curves from which precise dissociation constants (K$_{d}$) for protein-peptide interactions ca…
View article: triSPIM: light sheet microscopy with isotropic super-resolution
triSPIM: light sheet microscopy with isotropic super-resolution Open
We propose a three-objective light sheet microscopy geometry which, through a combination of skewed lattice light sheet excitation through two objectives and the computational fusion of images taken from two separate lens pairings, would a…
View article: Research data supporting “Generation of strength in a drying colloidal dispersion: How the fracture toughness depends on film properties”
Research data supporting “Generation of strength in a drying colloidal dispersion: How the fracture toughness depends on film properties” Open
In experiments we generate images of cracks in drying suspensions The code determines the crack tip and then calculates the crack width as a function of distance from the crack tip.
View article: Terahertz correlation spectroscopy infers particle velocity and rheological properties
Terahertz correlation spectroscopy infers particle velocity and rheological properties Open
Correlation spectroscopy is an analytical technique that can identify the residence time of reflective or fluorescent particles in a measurement spot, allowing particle velocity or diffusion to be inferred. We show that the technique can b…