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View article: Leukocytes use endothelial membrane tunnels to extravasate the vasculature
Leukocytes use endothelial membrane tunnels to extravasate the vasculature Open
Upon inflammation, leukocytes extravasate through endothelial cells. When they extravasate, it is generally accepted that neighboring endothelial cells disconnect. Careful examination of endothelial junctions showed a partial membrane over…
View article: A live tumor fragment platform to assess immunotherapy response in core needle biopsies while addressing challenges of tumor heterogeneity
A live tumor fragment platform to assess immunotherapy response in core needle biopsies while addressing challenges of tumor heterogeneity Open
Background Immune checkpoint inhibitors (ICIs) have revolutionized cancer treatment, providing durable and even curative responses. However, most patients do not respond and current biomarkers (eg, programmed death ligand (PD-L1), mismatch…
View article: Leukocytes use endothelial membrane tunnels to extravasate the vasculature
Leukocytes use endothelial membrane tunnels to extravasate the vasculature Open
Upon inflammation, leukocytes extravasate through endothelial cells. When they extravasate in a paracellular manner, it is generally accepted that neighbouring endothelial cells physically disconnect to open cell-cell junctions, allowing l…
View article: Distinct mobility patterns of BRCA2 molecules at DNA damage sites
Distinct mobility patterns of BRCA2 molecules at DNA damage sites Open
BRCA2 is an essential tumor suppressor protein involved in promoting faithful repair of DNA lesions. The activity of BRCA2 needs to be tuned precisely to be active when and where it is needed. Here, we quantified the spatio-temporal dynami…
View article: Label-free fluorescence lifetime imaging for the assessment of cell viability in living tumor fragments
Label-free fluorescence lifetime imaging for the assessment of cell viability in living tumor fragments Open
These data demonstrate that MP-FLIM can detect and quantify cell viability without the use of potentially toxic dyes, thus enabling longitudinal multi-day studies assessing the effects of therapeutic agents on tumor fragments.
View article: Four-dimensional quantitative analysis of cell plate development in Arabidopsis using lattice light sheet microscopy identifies robust transition points between growth phases
Four-dimensional quantitative analysis of cell plate development in Arabidopsis using lattice light sheet microscopy identifies robust transition points between growth phases Open
Cell plate formation during cytokinesis entails multiple stages occurring concurrently and requiring orchestrated vesicle delivery, membrane remodelling, and timely deposition of polysaccharides, such as callose. Understanding such a dynam…
View article: Assessing cell viability with dynamic optical coherence microscopy
Assessing cell viability with dynamic optical coherence microscopy Open
Assessing cell viability is important in many fields of research. Current optical methods to assess cell viability typically involve fluorescent dyes, which are often less reliable and have poor permeability in primary tissues. Dynamic opt…
View article: Distinct mobility patterns of BRCA2 molecules at DNA damage sites v2
Distinct mobility patterns of BRCA2 molecules at DNA damage sites v2 Open
This repository contains source data and processed data accompanied with the paper: "Distinct mobility patterns of BRCA2 molecules at DNA damage sites" The repository contains the following files: single molecule tracking data of BRCA2-Hal…
View article: Distinct mobility patterns of BRCA2 molecules at DNA damage sites v2
Distinct mobility patterns of BRCA2 molecules at DNA damage sites v2 Open
This repository contains source data and processed data accompanied with the paper: "Distinct mobility patterns of BRCA2 molecules at DNA damage sites" The repository contains the following files: single molecule tracking data of BRCA2-Hal…
View article: 123 Assessing cytotoxic T cell responses in live tumor fragments (LTF)™ with multiphoton microscopy
123 Assessing cytotoxic T cell responses in live tumor fragments (LTF)™ with multiphoton microscopy Open
Background Immune Checkpoint Inhibitors (ICI) have revolutionized cancer treatment. Unfortunately, existing FDA approved biomarkers like PD-L1, MSI and dMMR do not accurately predict which patients will respond. The Elephas Cybrid™ platfor…
View article: Four-dimensional quantitative analysis of cell plate development using lattice light sheet microscopy identifies robust transition points between growth phases
Four-dimensional quantitative analysis of cell plate development using lattice light sheet microscopy identifies robust transition points between growth phases Open
Cell plate formation during cytokinesis entails multiple stages occurring concurrently and requiring orchestrated vesicle delivery, membrane remodeling, and timely polysaccharide deposition, such as callose. Such a dynamic process requires…
View article: Dissecting Cell Plate Development During Plant Cytokinesis
Dissecting Cell Plate Development During Plant Cytokinesis Open
Journal Article Dissecting Cell Plate Development During Plant Cytokinesis Get access Rosalie Sinclair, Rosalie Sinclair University of California, Davis, United States Search for other works by this author on: Oxford Academic Google Schola…
View article: Novel Machine Vision Image Guidance System Significantly Reduces Procedural Time and Radiation Exposure Compared With 2-dimensional Fluoroscopy-based Guidance in Pediatric Deformity Surgery
Novel Machine Vision Image Guidance System Significantly Reduces Procedural Time and Radiation Exposure Compared With 2-dimensional Fluoroscopy-based Guidance in Pediatric Deformity Surgery Open
Background: Intraoperative 2-dimensional (2D) fluoroscopy imaging has been commonly adopted for guidance during complex pediatric spinal deformity correction. Despite the benefits, fluoroscopy imaging emits harmful ionizing radiation, whic…
View article: Distinct mobility patterns of BRCA2 molecules at DNA damage sites
Distinct mobility patterns of BRCA2 molecules at DNA damage sites Open
BRCA2 is an essential tumor suppressor protein involved in promoting faithful repair of DNA lesions. The activity of BRCA2 needs to be tuned precisely to be active when and where it is needed. Here, we quantified the spatio-temporal dynami…
View article: Distinct mobility patterns of BRCA2 molecules at DNA damage sites
Distinct mobility patterns of BRCA2 molecules at DNA damage sites Open
This repository contains source data and processed data accompanied with the paper: "Distinct mobility patterns of BRCA2 molecules at DNA damage sites" The repository contains the following files: - Movies: This folder contains the movies …
View article: Binding partners regulate unfolding of myosin VI to activate the molecular motor
Binding partners regulate unfolding of myosin VI to activate the molecular motor Open
Myosin VI is the only minus-end actin motor and it is coupled to various cellular processes ranging from endocytosis to transcription. This multi-potent nature is achieved through alternative isoform splicing and interactions with a networ…
View article: Quantifying Molecular Dynamics within Complex Cellular Morphologies using LLSM‐FRAP
Quantifying Molecular Dynamics within Complex Cellular Morphologies using LLSM‐FRAP Open
Quantifying molecular dynamics within the context of complex cellular morphologies is essential toward understanding the inner workings and function of cells. Fluorescence recovery after photobleaching (FRAP) is one of the most broadly app…
View article: Endothelial junctional membrane protrusions serve as hotspots for neutrophil transmigration
Endothelial junctional membrane protrusions serve as hotspots for neutrophil transmigration Open
Upon inflammation, leukocytes rapidly transmigrate across the endothelium to enter the inflamed tissue. Evidence accumulates that leukocytes use preferred exit sites, alhough it is not yet clear how these hotspots in the endothelium are de…
View article: Endothelial junctional membrane protrusions serve as hotspots for neutrophil transmigration
Endothelial junctional membrane protrusions serve as hotspots for neutrophil transmigration Open
Upon inflammation, leukocytes rapidly transmigrate across the endothelium to enter the inflamed tissue. Evidence accumulates that leukocytes use preferred exit sites, though it is not yet clear how these hotspots in the endothelium are def…
View article: Binding partners regulate unfolding of myosin VI to activate the molecular motor
Binding partners regulate unfolding of myosin VI to activate the molecular motor Open
Myosin VI is the only minus-end actin motor and is coupled to various cellular processes ranging from endocytosis to transcription. This multi-potent nature is achieved through alternative isoform splicing and interactions with a network o…
View article: Nuclear myosin VI regulates the spatial organization of mammalian transcription initiation
Nuclear myosin VI regulates the spatial organization of mammalian transcription initiation Open
SUMMARY During transcription, RNA Polymerase II (RNAPII) is spatially organised within the nucleus into clusters that correlate with transcription activity. While this is a hallmark of genome regulation in mammalian cells, the mechanisms c…
View article: FMNL2 regulates dynamics of fascin in filopodia
FMNL2 regulates dynamics of fascin in filopodia Open
Filopodia are peripheral F-actin–rich structures that enable cell sensing of the microenvironment. Fascin is an F-actin–bundling protein that plays a key role in stabilizing filopodia to support efficient adhesion and migration. Fascin is …
View article: Hydra image processor: 5-D GPU image analysis library with MATLAB and python wrappers
Hydra image processor: 5-D GPU image analysis library with MATLAB and python wrappers Open
Summary Light microscopes can now capture data in five dimensions at very high frame rates producing terabytes of data per experiment. Five-dimensional data has three spatial dimensions (x, y, z), multiple channels (λ) and time (t). Curren…
View article: The Complete Interactome with 5-D GPU Accelerated Analysis, Visualization, and UI for Biological Microscopy Applications
The Complete Interactome with 5-D GPU Accelerated Analysis, Visualization, and UI for Biological Microscopy Applications Open
Microscopes now have the ability to collect highly complex data. This thesis presents new algorithms and software packages for analyzing, visualizing, and interacting with biological microscopy image datasets. These new software packages p…