Daniel E. Otzen
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View article: Nitrated products formed on α-synuclein are preferentially incorporated into oligomers but excluded from fibrils: A mechanism for accumulation of neurotoxic species
Nitrated products formed on α-synuclein are preferentially incorporated into oligomers but excluded from fibrils: A mechanism for accumulation of neurotoxic species Open
View article: Environmental identification of novel enzymes against heteroatomic plastics
Environmental identification of novel enzymes against heteroatomic plastics Open
Better enzymes are needed to develop sustainable methods to recycle plastics with C-X heterobonds such as polyurethane (PUR) and nylon, for which no industrial-scale solutions exist. Current methods rely largely on sequence mining based on…
View article: The Importance of Being Imperfect: Structure and Function of Bacterial Amyloid
The Importance of Being Imperfect: Structure and Function of Bacterial Amyloid Open
Amyloids, once viewed solely as pathological hallmarks, are now recognized as widespread and versatile functional protein assemblies. Bacterial functional amyloids (FuBAs), particularly curli (CsgA) from Escherichia coli and FapC from Pseu…
View article: Homologous <scp>HipA</scp> ‐like kinases are controlled by internal translational initiation and genetic organisation
Homologous <span>HipA</span> ‐like kinases are controlled by internal translational initiation and genetic organisation Open
HipA‐like kinases are widespread bacterial serine–threonine kinases, yet their regulatory mechanisms remain poorly understood. Here, we characterise two novel HipA‐like systems, the monocistronic hipL and bicistronic hipIN , also encoding …
View article: Divergent effects of anionic surfactants on laundry enzymes: Structural stability, activity modulation, and self-cleavage mechanisms
Divergent effects of anionic surfactants on laundry enzymes: Structural stability, activity modulation, and self-cleavage mechanisms Open
Research on interactions between surfactants and proteins typically studies protein denaturation. Resistance to surfactant-induced denaturation is exploited in the use of detergent enzymes which are commonly used with petrochemically deriv…
View article: PhaseXplorer Creates High-Dimensional Phase Diagrams with Closed-Loop Active Learning
PhaseXplorer Creates High-Dimensional Phase Diagrams with Closed-Loop Active Learning Open
Phase separation fundamentally governs material properties and cellular function on multiple organizational scales. Conventional approaches to studying this nonlinear phenomenon necessitate resource-intensive experiments. As such, investig…
View article: Dynamic visualization of extracellular matrix components in S. aureus colony biofilms reveals functional amyloids leading to the formation of cap-like structures
Dynamic visualization of extracellular matrix components in S. aureus colony biofilms reveals functional amyloids leading to the formation of cap-like structures Open
View article: Single-vesicle Tracking of α-Synuclein Oligomers Reveals Pore Formation by a Three-Stage Model
Single-vesicle Tracking of α-Synuclein Oligomers Reveals Pore Formation by a Three-Stage Model Open
Neurodegenerative disorders, such as Parkinson's disease (PD) pose significant health challenges. A major hallmark of PD is the aggregation of α-synuclein into toxic oligomers (αSO) and fibrils. While many efforts focus on slowing disease …
View article: <i>Helicobacter pylori</i> CagA protein is a potent and broad-spectrum amyloid inhibitor
<i>Helicobacter pylori</i> CagA protein is a potent and broad-spectrum amyloid inhibitor Open
Bacteria, the smallest and most abundant life forms on Earth, have been a source of insights that have had a considerable impact on human health. Helicobacter pylori has captured substantial attention due to its role in provoking an array …
View article: Natural Design of a Stabilized Cross‐β Fold: Structure of the FuA <i>FapC</i> from <i>Pseudomonas</i> Sp. UK4 Reveals a Critical Role for Stacking of Imperfect Repeats
Natural Design of a Stabilized Cross‐β Fold: Structure of the FuA <i>FapC</i> from <i>Pseudomonas</i> Sp. UK4 Reveals a Critical Role for Stacking of Imperfect Repeats Open
An essential structural component of bacterial biofilms is functional amyloid (FuA), which also has great potential as an engineerable nano‐biomaterial. However, experimentally based high resolution structures of FuA that resolve individua…
View article: Nanobodies raised against the cytotoxic α-synuclein oligomer are oligomer-specific and promote its cellular uptake
Nanobodies raised against the cytotoxic α-synuclein oligomer are oligomer-specific and promote its cellular uptake Open
Parkinson’s disease involves the accumulation of aggregates of ɑ-synuclein (ɑ-Syn), both as intracellular fibrils and as cytotoxic soluble oligomeric species (ɑSOs). No available nanobodies show exclusive preference for the oligomeric stat…
View article: On the Hydrolysis of the Urethane Bond Catalyzed by Pseudomonas sp. MIS38 Lipase: a QM/MM Mechanistic Insight
On the Hydrolysis of the Urethane Bond Catalyzed by Pseudomonas sp. MIS38 Lipase: a QM/MM Mechanistic Insight Open
Plastic accumulation has become a major global concern, due to the lack of efficient and environmentally friendly strategies to manage the end-of-life of these materials. Among the most used families of plastics is polyurethane (PU), value…
View article: Quaternary stabilization of a <scp>GH2</scp><i>β</i>‐galactosidase from the psychrophile <i>A. ikkensis</i>, a flexible and unstable dimeric enzyme
Quaternary stabilization of a <span>GH2</span><i>β</i>‐galactosidase from the psychrophile <i>A. ikkensis</i>, a flexible and unstable dimeric enzyme Open
Studies of cold‐active enzymes may elucidate the basis for low‐temperature activity and contribute to their wider application in energy‐efficient processes. Here we investigate the cold‐active GH2 β ‐galactosidase from the psychrophilic ba…
View article: Role of electrostatics in cold adaptation: A comparative study of eury- and stenopsychrophilic triose phosphate isomerase
Role of electrostatics in cold adaptation: A comparative study of eury- and stenopsychrophilic triose phosphate isomerase Open
Psychrophilic (cold-active) organisms have developed enzymes that facilitate sufficient metabolic activity at low temperatures to sustain life. This occurs through molecular adaptations that tend to increase protein flexibility at the expe…
View article: Single-vesicle tracking of α-synuclein oligomers reveals pore formation by a three-stage model modulated by charge, curvature, lipids and ligands
Single-vesicle tracking of α-synuclein oligomers reveals pore formation by a three-stage model modulated by charge, curvature, lipids and ligands Open
Neurodegenerative disorders like Parkinson’s disease (PD) pose significant health challenges. A major hallmark is the aggregation of α-synuclein into toxic oligomers (αSO) and fibrils. While many efforts focus on slowing disease progressio…
View article: Cold-Active Starch-Degrading Enzymes from a Cold and Alkaline Greenland Environment: Role of Ca2+ Ions and Conformational Dynamics in Psychrophilicity
Cold-Active Starch-Degrading Enzymes from a Cold and Alkaline Greenland Environment: Role of Ca2+ Ions and Conformational Dynamics in Psychrophilicity Open
Cold-active enzymes hold promise for energy-efficient processes. Amylases are widely used in household and industrial applications, but only a few are cold-active. Here we describe three novel secreted amylases, Rho13, Ika2 and I3C6, all f…
View article: Investigating strategies for creating cross-linked amyloid fibril networks through branching of amyloid growth
Investigating strategies for creating cross-linked amyloid fibril networks through branching of amyloid growth Open
View article: Interactions between pathological and functional amyloid: A match made in Heaven or Hell?
Interactions between pathological and functional amyloid: A match made in Heaven or Hell? Open
The amyloid state of proteins occurs in many different contexts in Nature and in modern society, ranging from the pathological kind (neurodegenerative diseases and amyloidosis) via man-made forms (food processing and - to a much smaller ex…
View article: Cold-Active Starch-Degrading Enzymes from a Cold and Alkaline Greenland Environment: Role of Ca<sup>2+</sup> Ions and Conformational Dynamics in Psychrophilicity
Cold-Active Starch-Degrading Enzymes from a Cold and Alkaline Greenland Environment: Role of Ca<sup>2+</sup> Ions and Conformational Dynamics in Psychrophilicity Open
Cold-active enzymes hold promise in energy-efficient processes. Amylases are widely used in household and industrial applications, but only a few are cold-active. Here we describe three novel secreted amylases Rho13, Ika2 and I3C6, all fro…
View article: Strategies for Creating Cross-Linked Amyloid Fibril Networks Through Passive Branching of Amyloid Growth
Strategies for Creating Cross-Linked Amyloid Fibril Networks Through Passive Branching of Amyloid Growth Open
View article: Transforming Computational Power into Environmental Solutions: HPC-driven Research on Urethanase-mediated Plastic Degradation
Transforming Computational Power into Environmental Solutions: HPC-driven Research on Urethanase-mediated Plastic Degradation Open
View article: Resilience and charge-dependent fibrillation of functional amyloid: Interactions of Pseudomonas biofilm-associated FapB and FapC amyloids
Resilience and charge-dependent fibrillation of functional amyloid: Interactions of Pseudomonas biofilm-associated FapB and FapC amyloids Open
FapC and FapB are biofilm-associated amyloids involved in the virulence of Pseudomonas and other bacteria. We herein demonstrate their exceptional thermal and chemical resilience, suggesting that their biofilm structures might withstand st…
View article: Unveiling the enzymatic pathway of UMG-SP2 urethanase: insights into polyurethane degradation at the atomic level
Unveiling the enzymatic pathway of UMG-SP2 urethanase: insights into polyurethane degradation at the atomic level Open
This work uncovers the detailed catalytic mechanism of UMG-SP2 urethanase, offering insights into improving PU degradation.
View article: Dimethyl labeling of N-terminal amines allows unambiguous identification of protein crosslinks
Dimethyl labeling of N-terminal amines allows unambiguous identification of protein crosslinks Open
View article: Young rat microbiota extracts strongly inhibit fibrillation of α-synuclein and protect neuroblastoma cells and zebrafish against α-synuclein toxicity
Young rat microbiota extracts strongly inhibit fibrillation of α-synuclein and protect neuroblastoma cells and zebrafish against α-synuclein toxicity Open
View article: Magnetoresponsive liposomes applications in nanomedicine: A comprehensive review
Magnetoresponsive liposomes applications in nanomedicine: A comprehensive review Open
Safe and effective cancer therapy requires a suitable nanocarrier that can target particular sites, such as cancer cells, in a selective manner. With the tremendous growth in nanotechnology, liposomes, among various competing nanocarriers,…
View article: Investigating the interactions between an industrial lipase and anionic (bio)surfactants
Investigating the interactions between an industrial lipase and anionic (bio)surfactants Open
In laundry formulations, synergies between amphiphiles and other additives such as enzymes increase sustainability through a large decrease in energy consumption. However, traditional surfactants are derived from petroleum, requiring chemi…
View article: CsgA gatekeeper residues control nucleation but not stability of functional amyloid
CsgA gatekeeper residues control nucleation but not stability of functional amyloid Open
Functional amyloids, beneficial to the organism producing them, are found throughout life, from bacteria to humans. While disease‐related amyloids form by uncontrolled aggregation, the fibrillation of functional amyloid is regulated by com…
View article: Insight into protein crosslinking and casein polymerization in pre- and posthydrolyzed lactose-free ultra-high-temperature milk during long-term storage
Insight into protein crosslinking and casein polymerization in pre- and posthydrolyzed lactose-free ultra-high-temperature milk during long-term storage Open
During processing and storage of both conventional and lactose-hydrolyzed UHT milk (LHM), aggregation of milk proteins occurs. Protein aggregation can inter alia occur via nonreducible covalent cross-links derived from either Maillard or d…
View article: The Catalytic Mechanism of the Plastic-Degrading Enzyme Urethanase UMG-SP2
The Catalytic Mechanism of the Plastic-Degrading Enzyme Urethanase UMG-SP2 Open
The recently discovered metagenomic urethanases UMG-SP1, UMG-SP2, and UMG-SP3 have emerged as promising tools to improve existing chemical processes for polyurethane (PU) waste recycling. These enzymes are capable of breaking down urethane…