Erin Cutts
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View article: Condensin II activation by M18BP1
Condensin II activation by M18BP1 Open
Condensin I and II promote the drastic spatial rearrangement of the human genome upon mitotic entry. While condensin II is known to initiate this process in early mitosis, what triggers its activation and loading onto chromatin at this jun…
View article: Molecular mechanism of condensin I activation by KIF4A
Molecular mechanism of condensin I activation by KIF4A Open
During mitosis, the condensin I and II complexes compact chromatin into chromosomes. Loss of the chromokinesin, KIF4A, results in reduced condensin I association with chromosomes, but the molecular mechanism behind this phenotype is unknow…
View article: Herpes simplex virus type 1 origin binding protein UL9 tethers and loops origin- and non-origin-DNA intra- and intermolecularly
Herpes simplex virus type 1 origin binding protein UL9 tethers and loops origin- and non-origin-DNA intra- and intermolecularly Open
Herpesviruses are ubiquitous human pathogens, which are the causative agent of mild to severe symptoms ranging from cold sore to nasopharyngeal carcinoma. Even though replication of the linear dsDNA genome has been studied for decades, we …
View article: Condensin II activation by M18BP1
Condensin II activation by M18BP1 Open
Condensin complexes promote the drastic spatial rearrangement of the genome upon mitotic entry. Condensin II initiates chromosome condensation in early mitosis. To prevent chromosome condensation during interphase, condensin II is inhibite…
View article: Molecular Mechanism of Condensin I Activation by KIF4A
Molecular Mechanism of Condensin I Activation by KIF4A Open
Summary During mitosis, the condensin I and II complexes compact chromatin into chromosomes. Loss of the chromokinesin, KIF4A, results in reduced condensin I chromosome association. However, the molecular mechanism behind this phenotype is…
View article: CD8+ T Cells Mediate Lethal Lung Pathology in the Absence of PD-L1 and Type I Interferon Signalling following LCMV Infection
CD8+ T Cells Mediate Lethal Lung Pathology in the Absence of PD-L1 and Type I Interferon Signalling following LCMV Infection Open
CD8+ T cells are critical to the adaptive immune response against viral pathogens. However, overwhelming antigen exposure can result in their exhaustion, characterised by reduced effector function, failure to clear virus, and the upregulat…
View article: Substrate accessibility regulation of human TopIIa decatenation by cohesin
Substrate accessibility regulation of human TopIIa decatenation by cohesin Open
Human topoisomerase II alpha (TOP2α) is the main mitotic decatenase 1–3 resolving intertwines between sister chromatids that form during DNA replication 4 . Here, we employ quadruple-trap optical tweezers to generate braids between a pair …
View article: Author response: MCPH1 inhibits Condensin II during interphase by regulating its SMC2-Kleisin interface
Author response: MCPH1 inhibits Condensin II during interphase by regulating its SMC2-Kleisin interface Open
Article Figures and data Abstract Editor's evaluation Introduction Results Discussion Materials and methods Appendix 1 Data availability References Decision letter Author response Article and author information Metrics Abstract Dramatic ch…
View article: Condensin compacts DNA in 15 nm steps and requires FACT for extrusion on chromatin
Condensin compacts DNA in 15 nm steps and requires FACT for extrusion on chromatin Open
Condensin plays a central role in the organisation of chromosomes by compacting chromatin into loops during mitosis. Condensin achieves this through a loop extrusion mechanism that remains poorly understood. To identify the molecular steps…
View article: Linker histone H1.8 inhibits chromatin binding of condensins and DNA topoisomerase II to tune chromosome length and individualization
Linker histone H1.8 inhibits chromatin binding of condensins and DNA topoisomerase II to tune chromosome length and individualization Open
DNA loop extrusion by condensins and decatenation by DNA topoisomerase II (topo II) are thought to drive mitotic chromosome compaction and individualization. Here, we reveal that the linker histone H1.8 antagonizes condensins and topo II t…
View article: MCPH1 inhibits condensin II during interphase by regulating its SMC2-kleisin interface
MCPH1 inhibits condensin II during interphase by regulating its SMC2-kleisin interface Open
The dramatic change in morphology of chromosomal DNAs between interphase and mitosis is one of the defining features of the eukaryotic cell cycle. Two types of enzymes, namely cohesin and condensin confer the topology of chromosomal DNA by…
View article: A commercial antibody to the human condensin II subunit NCAPH2 cross-reacts with a SWI/SNF complex component
A commercial antibody to the human condensin II subunit NCAPH2 cross-reacts with a SWI/SNF complex component Open
Condensin complexes compact and disentangle chromosomes in preparation for cell division. Commercially available antibodies raised against condensin subunits have been widely used to characterise their cellular interactome. Here we have as…
View article: Linker histone H1.8 inhibits chromatin-binding of condensins and DNA topoisomerase II to tune chromosome length and individualization
Linker histone H1.8 inhibits chromatin-binding of condensins and DNA topoisomerase II to tune chromosome length and individualization Open
Summary DNA loop extrusion by condensins and decatenation by DNA topoisomerase II (topo II) are thought to drive mitotic chromosome compaction and individualization. Here, we reveal that the linker histone H1.8 antagonizes condensins and t…
View article: A commercial antibody to the human condensin II subunit NCAPH2 cross-reacts with a SWI/SNF complex component
A commercial antibody to the human condensin II subunit NCAPH2 cross-reacts with a SWI/SNF complex component Open
Summary Condensin complexes compact and disentangle chromosomes in preparation for cell division. Commercially available antibodies raised against condensin subunits have been widely used to characterise their cellular interactome. Here we…
View article: Condensin complexes: understanding loop extrusion one conformational change at a time
Condensin complexes: understanding loop extrusion one conformational change at a time Open
Condensin and cohesin, both members of the structural maintenance of chromosome (SMC) family, contribute to the regulation and structure of chromatin. Recent work has shown both condensin and cohesin extrude DNA loops and most likely work …
View article: A quantitative binding model for the Apl protein, the dual purpose recombination-directionality factor and lysis-lysogeny regulator of bacteriophage 186
A quantitative binding model for the Apl protein, the dual purpose recombination-directionality factor and lysis-lysogeny regulator of bacteriophage 186 Open
The Apl protein of bacteriophage 186 functions both as an excisionase and as a transcriptional regulator; binding to the phage attachment site (att), and also between the major early phage promoters (pR-pL). Like other recombination direct…
View article: Human condensin I and II drive extensive ATP-dependent compaction of nucleosome-bound DNA. Kong and Cutts et al
Human condensin I and II drive extensive ATP-dependent compaction of nucleosome-bound DNA. Kong and Cutts et al Open
This dataset contains raw data used in generating figures in the article “Human condensin I and II drive extensive ATP-dependent compaction of nucleosome-bound DNA" by Kong and Cutts et al. Kymographs and movies (TIFF stacks) from sin…
View article: A quantitative binding model for the Apl protein, the dual purpose recombination-directionality factor and lysis-lysogeny regulator of bacteriophage 186
A quantitative binding model for the Apl protein, the dual purpose recombination-directionality factor and lysis-lysogeny regulator of bacteriophage 186 Open
The Apl protein of bacteriophage 186 functions both as an excisionase and as a transcriptional regulator; binding to the phage attachment site ( att ), and also between the major early phage promoters (pR-pL). Like other recombination dire…
View article: Human condensin I and II drive extensive ATP–dependent compaction of nucleosome–bound DNA
Human condensin I and II drive extensive ATP–dependent compaction of nucleosome–bound DNA Open
Structural maintenance of chromosomes (SMC) complexes are essential for genome organization from bacteria to humans, but their mechanisms of action remain poorly understood. Here, we characterize human SMC complexes condensin I and II and …
View article: Troubleshooting biGBac: a practical guide v1
Troubleshooting biGBac: a practical guide v1 Open
The biGBac system presented by Weissmann et al. (Weissmann et al, 2016 and Weissmann et al, 2018) is a modular insect cell expression system designed for expression of multi-subunit complexes. The system is very powerful, allowing up to 25…
View article: Structural analysis of P. falciparum KAHRP and PfEMP1 complexes with host erythrocyte spectrin suggests a model for cytoadherent knob protrusions
Structural analysis of P. falciparum KAHRP and PfEMP1 complexes with host erythrocyte spectrin suggests a model for cytoadherent knob protrusions Open
Plasmodium falciparum Erythrocyte Membrane Protein 1 (PfEMP1) and Knob-associated Histidine-rich Protein (KAHRP) are directly linked to malaria pathology. PfEMP1 and KAHRP cluster on protrusions (knobs) on the P. falciparum-infected erythr…
View article: <i>Plasmodium falciparum Plasmodium</i>helical interspersed subtelomeric proteins contribute to cytoadherence and anchor<i>P. falciparum</i>erythrocyte membrane protein 1 to the host cell cytoskeleton
<i>Plasmodium falciparum Plasmodium</i>helical interspersed subtelomeric proteins contribute to cytoadherence and anchor<i>P. falciparum</i>erythrocyte membrane protein 1 to the host cell cytoskeleton Open
Adherence of Plasmodium falciparum-infected erythrocytes to host endothelium is conferred through the parasite-derived virulence factor P. falciparum erythrocyte membrane protein 1 (PfEMP1), the major contributor to malaria severity. PfEMP…
View article: Plasmodium falciparum PHIST Proteins Contribute to Cytoadherence and Anchor PfEMP1 to the Host Cell Cytoskeleton
Plasmodium falciparum PHIST Proteins Contribute to Cytoadherence and Anchor PfEMP1 to the Host Cell Cytoskeleton Open
Adherence of Plasmodium falciparum infected erythrocytes to host endothelium is conferred through the parasite-derived virulence factor PfEMP1, the major contributor to malaria severity. PfEMP1 located at knob structures on the erythrocyte…
View article: Towards a structural model of adherent knobs on the surface of Plasmodium falciparum infected erythrocytes
Towards a structural model of adherent knobs on the surface of Plasmodium falciparum infected erythrocytes Open
The Plasmodium falciparum proteome is enriched in intrinsically disordered proteins, three of which are directly linked to malaria severity; P. falciparum Erythrocyte Membrane Protein 1 (Pf EMP1), Knob-associated Histidine-rich Protein (KA…