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View article: Linker histone regulates the myeloid versus lymphoid bifurcation of multipotent hematopoietic stem and progenitors
Linker histone regulates the myeloid versus lymphoid bifurcation of multipotent hematopoietic stem and progenitors Open
Myeloid-biased differentiation of multipotent hematopoietic stem and progenitor cells (HSPCs) occurs with aging or exhaustion. The molecular mechanism(s) responsible for this fate bias remain unclear. Here, we report that linker histone re…
View article: Sclerotic GVHD and Scleroderma Share Dysregulated Gene Expression that is Ameliorated by EREG Therapeutic Antibody
Sclerotic GVHD and Scleroderma Share Dysregulated Gene Expression that is Ameliorated by EREG Therapeutic Antibody Open
Immune driven fibrotic skin diseases including scleroderma/systemic sclerosis (SSc) and chronic graft-versus-host-disease (cGvHD) cause skin stiffening that has major impact on patient quality of life and associated patient mortality. Ther…
View article: Humanization of CD47 enables development of functional human neutrophils via postirradiation remodeling of the bone marrow
Humanization of CD47 enables development of functional human neutrophils via postirradiation remodeling of the bone marrow Open
Murine and human immune systems differ significantly, particularly within the myeloid lineage. Humanized mice, generated by transplanting human hematopoietic stem, progenitor cells into genetically modified mice, are invaluable to study hu…
View article: Engineering Mice to Study Human Immunity
Engineering Mice to Study Human Immunity Open
Humanized mice, which carry a human hematopoietic and immune system, have greatly advanced our understanding of human immune responses and immunological diseases. These mice are created via the transplantation of human hematopoietic stem a…
View article: Linker histone regulates the myeloid versus lymphoid bifurcation of multipotent hematopoietic stem and progenitors
Linker histone regulates the myeloid versus lymphoid bifurcation of multipotent hematopoietic stem and progenitors Open
Myeloid-biased differentiation of multipotent hematopoietic stem and progenitor cells (HSPCs) occurs with aging or exhaustion. The molecular mechanism(s) responsible for this fate bias remain unclear. Here we report that linker histone reg…
View article: The human CD47 checkpoint is targeted by an immunosuppressive <i>Aedes aegypti</i> salivary factor to enhance arboviral skin infectivity
The human CD47 checkpoint is targeted by an immunosuppressive <i>Aedes aegypti</i> salivary factor to enhance arboviral skin infectivity Open
The Aedes aegypti mosquito is a vector of many infectious agents, including flaviviruses such as Zika virus. Components of mosquito saliva have pleomorphic effects on the vertebrate host to enhance blood feeding, and these changes also cre…
View article: IL-4 Licenses B Cell Activation Through Cholesterol Synthesis
IL-4 Licenses B Cell Activation Through Cholesterol Synthesis Open
Lymphocyte activation involves a transition from quiescence and associated catabolic metabolism to a metabolic state with noted similarities to cancer cells such as heavy reliance on aerobic glycolysis for energy demands and increased nutr…
View article: Gene trajectory inference for single-cell data by optimal transport metrics
Gene trajectory inference for single-cell data by optimal transport metrics Open
View article: Scaling deep identifiable models enables zero-shot characterization of single-cell biological states
Scaling deep identifiable models enables zero-shot characterization of single-cell biological states Open
How to identify true biological differences across samples while overcoming batch effects has been a persistent challenge in single-cell RNA-seq data analysis, hindering analyses across datasets for transferable biological findings. In thi…
View article: 1025 Tumor-specific CD8+ T cells epigenetically licensed by IL-7R are critical for anti-tumor immunity in melanoma
1025 Tumor-specific CD8+ T cells epigenetically licensed by IL-7R are critical for anti-tumor immunity in melanoma Open
Background Recurrence of advanced melanoma after checkpoint therapy is a major risk factor for death, and treatment options are limited. Anti-tumor memory plays a critical role in preventing recurrence, but the identity, location and funct…
View article: IL-6 trans-signaling in a humanized mouse model of scleroderma
IL-6 trans-signaling in a humanized mouse model of scleroderma Open
Fibrosis is regulated by interactions between immune and mesenchymal cells. However, the capacity of cell types to modulate human fibrosis pathology is poorly understood due to lack of a fully humanized model system. MISTRG6 mice were engi…
View article: Humanized mouse liver reveals endothelial control of essential hepatic metabolic functions
Humanized mouse liver reveals endothelial control of essential hepatic metabolic functions Open
View article: IL-7R licenses a population of epigenetically poised memory CD8 <sup>+</sup> T cells with superior antitumor efficacy that are critical for melanoma memory
IL-7R licenses a population of epigenetically poised memory CD8 <sup>+</sup> T cells with superior antitumor efficacy that are critical for melanoma memory Open
Recurrence of advanced melanoma after therapy is a major risk factor for reduced survival, and treatment options are limited. Antitumor immune memory plays a critical role in preventing melanoma recurrence and memory T cells could be a pot…
View article: Autologous humanized PDX modeling for immuno-oncology recapitulates features of the human tumor microenvironment
Autologous humanized PDX modeling for immuno-oncology recapitulates features of the human tumor microenvironment Open
Background Interactions between immune and tumor cells are critical to determining cancer progression and response. In addition, preclinical prediction of immune-related drug efficacy is limited by interspecies differences between human an…
View article: Human neutrophil development and functionality are enabled in a humanized mouse model
Human neutrophil development and functionality are enabled in a humanized mouse model Open
Mice with a functional human immune system serve as an invaluable tool to study the development and function of the human immune system in vivo. A major technological limitation of all current humanized mouse models is the lack of mature a…
View article: Autologous humanized PDX modeling for immuno-oncology recapitulates the human tumor microenvironment
Autologous humanized PDX modeling for immuno-oncology recapitulates the human tumor microenvironment Open
Interactions between immune and tumor cells are critical to determining cancer progression and response. In addition, preclinical prediction of immune-related drug efficacy is limited by inter-species differences between human and mouse, a…
View article: RNA m <sup>6</sup> A demethylase ALKBH5 regulates the development of γδ T cells
RNA m <sup>6</sup> A demethylase ALKBH5 regulates the development of γδ T cells Open
γδ T cells are an abundant T cell population at the mucosa and are important in providing immune surveillance as well as maintaining tissue homeostasis. However, despite γδ T cells’ origin in the thymus, detailed mechanisms regulating γδ T…
View article: Gene Trajectory Inference for Single-cell Data by Optimal Transport Metrics
Gene Trajectory Inference for Single-cell Data by Optimal Transport Metrics Open
Single-cell RNA-sequencing has been widely used to investigate cell state transitions and gene dynamics of biological processes. Current strategies to infer the sequential dynamics of genes in a process typically rely on constructing cell …
View article: Inflammasome activation in infected macrophages drives COVID-19 pathology
Inflammasome activation in infected macrophages drives COVID-19 pathology Open
View article: Human neutrophil development and functionality are enabled in a humanized mouse model
Human neutrophil development and functionality are enabled in a humanized mouse model Open
Mice with a functional human immune system serve as an invaluable tool to study the development and function of human immune system in vivo. A major technological limitation of all current humanized mouse models is the lack of mature and f…
View article: Viral replication in human macrophages enhances an inflammatory cascade and interferon driven chronic COVID-19 in humanized mice.
Viral replication in human macrophages enhances an inflammatory cascade and interferon driven chronic COVID-19 in humanized mice. Open
In the MISTRG6 model of COVID-19, SARS-CoV-2 virus peaks at four days post infection (dpi) and then subsides to levels 1000-fold lower which persist for weeks. Similar to infected humans, viral RNA persists at high levels for several weeks…
View article: A humanized mouse model of chronic COVID-19
A humanized mouse model of chronic COVID-19 Open
View article: Inflammasome activation in infected macrophages drives COVID-19 pathology
Inflammasome activation in infected macrophages drives COVID-19 pathology Open
Severe COVID-19 is characterized by persistent lung inflammation, inflammatory cytokine production, viral RNA, and sustained interferon (IFN) response all of which are recapitulated and required for pathology in the SARS-CoV-2 infected MIS…
View article: Editorial: Regulatory T Cell Heterogeneity: Canonical and Non-Canonical Functions
Editorial: Regulatory T Cell Heterogeneity: Canonical and Non-Canonical Functions Open
EDITORIAL article Front. Immunol., 22 September 2021Sec. T Cell Biology Volume 12 - 2021 | https://doi.org/10.3389/fimmu.2021.722563
View article: Detection of differentially abundant cell subpopulations in scRNA-seq data
Detection of differentially abundant cell subpopulations in scRNA-seq data Open
Significance Comparative analysis of samples from two biological states, such as two stages of embryonic development, is a pressing problem in single-cell RNA sequencing (scRNA-seq). A key challenge is to detect cell subpopulations whose a…
View article: A humanized mouse model of chronic COVID-19 to evaluate disease mechanisms and treatment options
A humanized mouse model of chronic COVID-19 to evaluate disease mechanisms and treatment options Open
Coronavirus-associated acute respiratory disease, called coronavirus disease 2019 (COVID-19) is an infectious disease caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). More than 90 million people have been infected wi…
View article: Gene expression
Gene expression Open
We evaluated the transcriptional landscape at single cell or whole tissue level in uninfected and SARS-CoV-2 infected lungs of humanized mice at multiple time points (2, 4, 7, 14, 28 dpi).
View article: Immune cells
Immune cells Open
We characterized human and mouse immune cells in SARS-CoV-2 infected and uninfected MISTRG6-hACE2mice by flow cytometry to evaluate the cells that drive immunopathology and viral clearance.We reported frequencies (%) of cells at dif…
View article: Immune cells
Immune cells Open
We characterized human and mouse immune cells in SARS-CoV-2 infected and uninfected MISTRG6-hACE2mice by flow cytometry to evaluate the cells that drive immunopathology and viral clearance.We reported frequencies (%) of cells at dif…
View article: Gene expression
Gene expression Open
We evaluated the transcriptional landscape at single cell or whole tissue level in uninfected and SARS-CoV-2 infected lungs of humanized mice at multiple time points (2, 4, 7, 14, 28 dpi).