Etsuo A. Susaki
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View article: Whole-Brain Single-Neuron Atlas Reveals Microglial Security Hole Accelerating Neuronal Vulnerability
Whole-Brain Single-Neuron Atlas Reveals Microglial Security Hole Accelerating Neuronal Vulnerability Open
Neurodegenerative diseases are characterized by progressive neuronal loss, yet subtle degeneration remains difficult to detect, hindering advancements in early diagnosis and intervention strategies. Here, we present a 3D, whole-brain, sing…
View article: Whole‐brain Cellome‐Wide Association Study (CWAS) Unveils Ultra‐Early Neurodegeneration Phenomena in a 3D Spatial Cell Atlas
Whole‐brain Cellome‐Wide Association Study (CWAS) Unveils Ultra‐Early Neurodegeneration Phenomena in a 3D Spatial Cell Atlas Open
Background In aging societies, neurodegenerative diseases, such as Alzheimer’s disease, are receiving attention. These diseases are primary targets for preemptive medicine, emphasizing the importance of early detection and preventive treat…
View article: Whole‐brain Cellome‐Wide Association Study (CWAS) Unveils Ultra‐Early Neurodegeneration Phenomena in a 3D Spatial Cell Atlas
Whole‐brain Cellome‐Wide Association Study (CWAS) Unveils Ultra‐Early Neurodegeneration Phenomena in a 3D Spatial Cell Atlas Open
Background In aging societies, neurodegenerative diseases, such as Alzheimer's disease, are receiving attention. These diseases are primary targets for preemptive medicine, emphasizing the importance of early detection and preventive treat…
View article: Unlocking the potential of large-scale 3D imaging with tissue clearing techniques
Unlocking the potential of large-scale 3D imaging with tissue clearing techniques Open
The three-dimensional (3D) anatomical structure of living organisms is intrinsically linked to their functions, yet modern life sciences have not fully explored this aspect. Recently, the combination of efficient tissue clearing techniques…
View article: Cortical parvalbumin neurons are responsible for homeostatic sleep rebound through CaMKII activation
Cortical parvalbumin neurons are responsible for homeostatic sleep rebound through CaMKII activation Open
The homeostatic regulation of sleep is characterized by rebound sleep after prolonged wakefulness, but the molecular and cellular mechanisms underlying this regulation are still unknown. In this study, we show that Ca 2+ /calmodulin-depend…
View article: Signal improved ultra-fast light-sheet microscope for large tissue imaging
Signal improved ultra-fast light-sheet microscope for large tissue imaging Open
Axially swept light-sheet microscope in conjunction with tissue clearing enables three-dimensional morphological investigation of millimeter-scaled tissues at isotropic sub-micron resolution. However, these microscopes suffer from low dete…
View article: Blueprints from plane to space: outlook of next‐generation three‐dimensional histopathology
Blueprints from plane to space: outlook of next‐generation three‐dimensional histopathology Open
Here, we summarize the literature relevant to recent advances in three‐dimensional (3D) histopathology in relation to clinical oncology, highlighting serial sectioning, tissue clearing, light‐sheet microscopy, and digital image analysis wi…
View article: Bioorthogonal chemical labeling of endogenous neurotransmitter receptors in living mouse brains
Bioorthogonal chemical labeling of endogenous neurotransmitter receptors in living mouse brains Open
Neurotransmitter receptors are essential components of synapses for communication between neurons in the brain. Because the spatiotemporal expression profiles and dynamics of neurotransmitter receptors involved in many functions are delica…
View article: A novel tauopathy model mimicking molecular and spatial aspects of human tau pathology
A novel tauopathy model mimicking molecular and spatial aspects of human tau pathology Open
Creating a mouse model that recapitulates human tau pathology is essential for developing strategies to intervene in tau-induced neurodegeneration. However, mimicking the pathological features seen in human pathology often involves a trade…
View article: Signal Improved ultra-Fast Light-sheet Microscope (SIFT) for large tissue imaging
Signal Improved ultra-Fast Light-sheet Microscope (SIFT) for large tissue imaging Open
Light-sheet fluorescence microscopy (LSFM) in conjunction with tissue clearing techniques enables morphological investigation of large tissues faster and with excellent optical sectioning. Recently, cleared tissue axially swept light-sheet…
View article: Signal Improved ultra-Fast Light-sheet Microscope (SIFT) for large tissue imaging
Signal Improved ultra-Fast Light-sheet Microscope (SIFT) for large tissue imaging Open
Light-sheet fluorescence microscopy (LSFM) in conjunction with tissue clearing techniques enables morphological investigation of large tissues faster and with excellent optical sectioning. Recently, cleared tissue axially swept light-sheet…
View article: descSPIM: Affordable and Easy-to-Build Light-Sheet Microscopy for Tissue Clearing Technique Users
descSPIM: Affordable and Easy-to-Build Light-Sheet Microscopy for Tissue Clearing Technique Users Open
Summary Despite the easier use of multiple tissue clearing techniques in recent years, poor access to adequate light-sheet fluorescence microscopy remains a major obstacle for biomedical end users. Here, we propose a solution by developing…
View article: Cortical parvalbumin neurons are responsible for homeostatic sleep rebound through CaMKII activation
Cortical parvalbumin neurons are responsible for homeostatic sleep rebound through CaMKII activation Open
The homeostatic regulation of sleep is characterized by rebound sleep after prolonged wakefulness, but the molecular and cellular mechanisms underlying this regulation are still unknown. We show here that CaMKII-dependent activity control …
View article: Bioorthogonal chemical labelling of endogenous neurotransmitter receptors in living mouse brains
Bioorthogonal chemical labelling of endogenous neurotransmitter receptors in living mouse brains Open
Neurotransmitter receptors are essential components of synapses for communication between neurons in the brain. Because the spatiotemporal expression profiles and dynamics of neurotransmitter receptors involved in many functions are delica…
View article: Lipopolysaccharides and Cellular Senescence: Involvement in Atherosclerosis
Lipopolysaccharides and Cellular Senescence: Involvement in Atherosclerosis Open
Atherosclerosis is a chronic inflammatory disease of the vascular walls related to aging. Thus far, the roles of cellular senescence and bacterial infection in the pathogenesis of atherosclerosis have been speculated to be independent of e…
View article: Multiresolution nondestructive 3D pathology of whole lymph nodes for breast cancer staging
Multiresolution nondestructive 3D pathology of whole lymph nodes for breast cancer staging Open
For breast cancer patients, the extent of regional lymph node (LN) metastasis influences the decision to remove all axillary LNs. Metastases are currently identified and classified with visual analysis of a few thin tissue sections with co…
View article: Multiresolution nondestructive 3D pathology of whole lymph nodes for breast cancer staging.
Multiresolution nondestructive 3D pathology of whole lymph nodes for breast cancer staging. Open
Our 3D pathology method may improve clinical management for breast cancer patients by improving staging accuracy of LN metastases.
View article: A Novel Three-Dimensional Imaging System Based on Polysaccharide Staining for Accurate Histopathological Diagnosis of Inflammatory Bowel Diseases
A Novel Three-Dimensional Imaging System Based on Polysaccharide Staining for Accurate Histopathological Diagnosis of Inflammatory Bowel Diseases Open
PAFhy staining and PAFhy-3D imaging are promising approaches for next-generation experimental and clinical histopathology.
View article: A hybrid open-top light-sheet microscope for multi-scale imaging of cleared tissues
A hybrid open-top light-sheet microscope for multi-scale imaging of cleared tissues Open
Light-sheet microscopy has emerged as the preferred means for high-throughput volumetric imaging of cleared tissues. However, there is a need for a user-friendly system that can address imaging applications with varied requirements in term…
View article: Acoustic light-sheet microscopy
Acoustic light-sheet microscopy Open
Light-sheet imaging of 3D objects with high spatial resolution remains an open challenge because of the trade-off between field-of-view (FOV) and axial resolution originating from the diffraction of light. We developed acoustic light-sheet…
View article: Perspective: Extending the Utility of Three-Dimensional Organoids by Tissue Clearing Technologies
Perspective: Extending the Utility of Three-Dimensional Organoids by Tissue Clearing Technologies Open
An organoid, a self-organizing organ-like tissue developed from stem cells, can exhibit a miniaturized three-dimensional (3D) structure and part of the physiological functions of the original organ. Due to the reproducibility of tissue com…
View article: Activation of Sympathetic Signaling in Macrophages Blocks Systemic Inflammation and Protects against Renal Ischemia-Reperfusion Injury
Activation of Sympathetic Signaling in Macrophages Blocks Systemic Inflammation and Protects against Renal Ischemia-Reperfusion Injury Open
Significance Statement The detailed role of neural activity in inflammatory diseases is still unclear because it varies according to the disease situation and responsible cell types. This study shows that activation of β 2-adrenergic recep…
View article: CUBIC-Cloud: An Integrative Computational Framework Towards Community-driven Whole-Mouse-Brain Mapping
CUBIC-Cloud: An Integrative Computational Framework Towards Community-driven Whole-Mouse-Brain Mapping Open
Recent advancements in tissue clearing technologies have offered unparalleled opportunities for researchers to explore the whole mouse brain at cellular resolution. With the expansion of this experimental technique, however, a scalable and…
View article: A hybrid open-top light-sheet microscope for multi-scale imaging of cleared tissues
A hybrid open-top light-sheet microscope for multi-scale imaging of cleared tissues Open
Light-sheet microscopy has emerged as the preferred means for high-throughput volumetric imaging of cleared tissues. However, there is a need for a user-friendly system that can address imaging applications with varied requirements in term…
View article: Versatile whole-organ/body staining and imaging based on electrolyte-gel properties of biological tissues
Versatile whole-organ/body staining and imaging based on electrolyte-gel properties of biological tissues Open
Whole-organ/body three-dimensional (3D) staining and imaging have been enduring challenges in histology. By dissecting the complex physicochemical environment of the staining system, we developed a highly optimized 3D staining imaging pipe…