Euan Pyle
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View article: Cryo-electron tomography reveals how COPII assembles on cargo-containing membranes
Cryo-electron tomography reveals how COPII assembles on cargo-containing membranes Open
Proteins traverse the eukaryotic secretory pathway through membrane trafficking between organelles. The coat protein complex II (COPII) mediates the anterograde transport of newly synthesized proteins from the endoplasmic reticulum, engagi…
View article: An image processing pipeline for electron cryo‐tomography in <scp>RELION</scp> ‐5
An image processing pipeline for electron cryo‐tomography in <span>RELION</span> ‐5 Open
Electron tomography of frozen, hydrated samples allows structure determination of macromolecular complexes that are embedded in complex environments. Provided that the target complexes may be localised in noisy, three‐dimensional tomograph…
View article: An image processing pipeline for electron cryo-tomography in RELION-5
An image processing pipeline for electron cryo-tomography in RELION-5 Open
Electron tomography of frozen, hydrated samples allows structure determination of macromolecular complexes that are embedded in complex environments. Provided that the target complexes may be localised in noisy, three-dimensional tomograph…
View article: Cryo-electron tomography reveals how COPII assembles on cargo-containing membranes
Cryo-electron tomography reveals how COPII assembles on cargo-containing membranes Open
Proteins traverse the eukaryotic secretory pathway through membrane trafficking between organelles. The COPII coat mediates the anterograde transport of newly synthesised proteins from the endoplasmic reticulum, engaging cargoes with a wid…
View article: A Bayesian approach to single-particle electron cryo-tomography in RELION-4.0
A Bayesian approach to single-particle electron cryo-tomography in RELION-4.0 Open
We present a new approach for macromolecular structure determination from multiple particles in electron cryo-tomography (cryo-ET) data sets. Whereas existing subtomogram averaging approaches are based on 3D data models, we propose to opti…
View article: A Bayesian approach to single-particle electron cryo-tomography in RELION-4.0
A Bayesian approach to single-particle electron cryo-tomography in RELION-4.0 Open
We present a new approach for macromolecular structure determination from multiple particles in electron cryo-tomography (cryo-ET) data sets. Whereas existing subtomogram averaging approaches are based on 3D data models, we propose to opti…
View article: Strategies for picking membrane-associated particles within subtomogram averaging workflows
Strategies for picking membrane-associated particles within subtomogram averaging workflows Open
In cryo-tomography and subtomogram averaging, particle picking constitutes a major bottleneck and requires significant user time and expertise. Here we summarise particle-picking strategies, focusing in particular on membrane-associated pa…
View article: Current data processing strategies for cryo-electron tomography and subtomogram averaging
Current data processing strategies for cryo-electron tomography and subtomogram averaging Open
Cryo-electron tomography (cryo-ET) can be used to reconstruct three-dimensional (3D) volumes, or tomograms, from a series of tilted two-dimensional images of biological objects in their near-native states in situ or in vitro. 3D subvolumes…
View article: Specific Residues in a Purine Transporter Are Critical for Dimerization, ER Exit, and Function
Specific Residues in a Purine Transporter Are Critical for Dimerization, ER Exit, and Function Open
Transporters are transmembrane proteins that mediate the selective translocation of solutes across biological membranes. Recently, we have shown that specific interactions with plasma membrane phospholipids are essential for the formation …
View article: Insights into protein-lipid interactions by structural mass spectrometry
Insights into protein-lipid interactions by structural mass spectrometry Open
Interactions with membrane lipids have long been known to affect a wide range of membrane protein properties including folding, stability, and function. Our understanding of the precise molecular basis of these interactions is currently li…
View article: Distinct specific interactions of the UapA transporter with membrane lipids are critical for dimerization, ER-exit and function
Distinct specific interactions of the UapA transporter with membrane lipids are critical for dimerization, ER-exit and function Open
Transporters are transmembrane proteins that mediate the selective translocation of solutes across biological membranes. Recently, we have shown that specific interactions with plasma membrane phospholipids are essential for formation and/…
View article: HDX-MS reveals nucleotide-dependent, anti-correlated opening and closure of SecA and SecY channels of the bacterial translocon
HDX-MS reveals nucleotide-dependent, anti-correlated opening and closure of SecA and SecY channels of the bacterial translocon Open
The bacterial Sec translocon is a multi-protein complex responsible for translocating diverse proteins across the plasma membrane. For post-translational protein translocation, the Sec-channel – SecYEG – associates with the motor protein S…
View article: HDX-MS reveals nucleotide-regulated, anti-correlated opening and closure of SecA and SecY channels of the bacterial translocon
HDX-MS reveals nucleotide-regulated, anti-correlated opening and closure of SecA and SecY channels of the bacterial translocon Open
The bacterial Sec translocon is a multi-component protein complex responsible for translocating diverse proteins across the plasma membrane. For post-translational protein translocation, the Sec-channel – SecYEG – associates with the motor…
View article: ATP-induced asymmetric pre-protein folding as a driver of protein translocation through the Sec machinery
ATP-induced asymmetric pre-protein folding as a driver of protein translocation through the Sec machinery Open
Transport of proteins across membranes is a fundamental process, achieved in every cell by the ‘Sec’ translocon. In prokaryotes, SecYEG associates with the motor ATPase SecA to carry out translocation for pre-protein secretion. Previously,…
View article: Transporter oligomerisation: roles in structure and function
Transporter oligomerisation: roles in structure and function Open
Oligomerisation is a key feature of integral membrane transporters with roles in structure, function and stability. In this review, we cover some very recent advances in our understanding of how oligomerisation affects these key transporte…
View article: Specific cardiolipin–SecY interactions are required for proton-motive force stimulation of protein secretion
Specific cardiolipin–SecY interactions are required for proton-motive force stimulation of protein secretion Open
Significance Many proteins are located in lipid membranes surrounding cells and subcellular organelles. The membrane can impart important structural and functional effects on the protein, making understanding of this interaction critical. …
View article: Structural lipids enable the formation of functional oligomers of the eukaryotic purine symporter UapA
Structural lipids enable the formation of functional oligomers of the eukaryotic purine symporter UapA Open
The role of membrane lipids in modulating eukaryotic transporter structure and function remains poorly understood. We used native mass spectrometry in combination with molecular dynamics simulations and in vivo analyses to investigate the …
View article: Specific cardiolipin-SecY interactions are required for proton-motive-force stimulation of protein secretion
Specific cardiolipin-SecY interactions are required for proton-motive-force stimulation of protein secretion Open
The transport of proteins across or into membranes is a vital biological process, achieved in every cell by the conserved Sec machinery. In bacteria, SecYEG combines with the SecA motor protein for secretion of pre-proteins across the plas…
View article: Hybrid Mass Spectrometry: Towards Characterization of Protein Conformational States
Hybrid Mass Spectrometry: Towards Characterization of Protein Conformational States Open
A current challenge in structural biology is to unravel the conformational states of protein complexes. Hybrid mass spectrometry (MS) has emerged as a key tool to study the structural dynamics of large protein complexes unattainable by tra…