Eugene Rha
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View article: Dsembler - DNA Assembly Designer: A Tool for Facilitating Assembly of Oligomers
Dsembler - DNA Assembly Designer: A Tool for Facilitating Assembly of Oligomers Open
Synthetic biology has garnered significant global interest owing to its diverse applications in bio-based production, biosensing, living therapeutics, and drug delivery. This heightened interest has increased the demand for novel protein s…
View article: Cell-free biosensor with automated acoustic liquid handling for rapid and scalable characterization of cellobiohydrolases on microcrystalline cellulose
Cell-free biosensor with automated acoustic liquid handling for rapid and scalable characterization of cellobiohydrolases on microcrystalline cellulose Open
Engineering enzymes to degrade solid substrates, such as crystalline cellulose from paper sludge or microplastics in sewage sludge, presents challenges for high-throughput screening (HTS), as solid substrates are not readily accessible in …
View article: Engineered Methylococcus capsulatus Bath for efficient methane conversion to isoprene
Engineered Methylococcus capsulatus Bath for efficient methane conversion to isoprene Open
Isoprene has numerous industrial applications, including rubber polymer and potential biofuel. Microbial methane-based isoprene production could be a cost-effective and environmentally benign process, owing to a reduced carbon footprint an…
View article: A Genetically Encoded Biosensor for the Detection of Levulinic Acid
A Genetically Encoded Biosensor for the Detection of Levulinic Acid Open
Levulinic acid (LA) is a valuable chemical used in fuel additives, fragrances, and polymers. In this study, we proposed possible biosynthetic pathways for LA production from lignin and poly(ethylene terephthalate). We also created a geneti…
View article: CRISPRi-based programmable logic inverter cascade for antibiotic-free selection and maintenance of multiple plasmids
CRISPRi-based programmable logic inverter cascade for antibiotic-free selection and maintenance of multiple plasmids Open
Antibiotics have been widely used for plasmid-mediated cell engineering. However, continued use of antibiotics increases the metabolic burden, horizontal gene transfer risks, and biomanufacturing costs. There are limited approaches to main…
View article: Novel High-Throughput DNA Part Characterization Technique for Synthetic Biology
Novel High-Throughput DNA Part Characterization Technique for Synthetic Biology Open
This study presents a novel DNA part characterization technique that increases throughput by combinatorial DNA part assembly, solid plate-based quantitative fluorescence assay for phenotyping, and barcode tagging-based long-read sequencing…
View article: A Highly Efficient and Versatile Genetic Engineering Toolkit For a Methanotroph-Based Biorefinery
A Highly Efficient and Versatile Genetic Engineering Toolkit For a Methanotroph-Based Biorefinery Open
View article: Antagonistic Control of Genetic Circuit Performance for Rapid Analysis of Targeted Enzyme Activity in Living Cells
Antagonistic Control of Genetic Circuit Performance for Rapid Analysis of Targeted Enzyme Activity in Living Cells Open
Genetic circuits have been developed for quantitative measurement of enzyme activity, metabolic engineering of strain development, and dynamic regulation of microbial cells. A genetic circuit consists of several bio-elements, including enz…
View article: Machine learning linked evolutionary biosensor array for highly sensitive and specific molecular identification
Machine learning linked evolutionary biosensor array for highly sensitive and specific molecular identification Open
View article: Sensitive and Rapid Phenotyping of Microbes With Soluble Methane Monooxygenase Using a Droplet-Based Assay
Sensitive and Rapid Phenotyping of Microbes With Soluble Methane Monooxygenase Using a Droplet-Based Assay Open
Methanotrophs with soluble methane monooxygenase (sMMO) show high potential for various ecological and biotechnological applications. Here, we developed a high throughput method to identify sMMO-producing microbes by integrating droplet mi…
View article: Acclimation of bacterial cell state for high-throughput enzyme engineering using a DmpR-dependent transcriptional activation system
Acclimation of bacterial cell state for high-throughput enzyme engineering using a DmpR-dependent transcriptional activation system Open
Genetic circuit-based biosensors have emerged as an effective analytical tool in synthetic biology; these biosensors can be applied to high-throughput screening of new biocatalysts and metabolic pathways. Sigma 54 (σ 54 )-dependent transcr…
View article: Discovery and Biochemical Characterization of a Methanol Dehydrogenase From Lysinibacillus xylanilyticus
Discovery and Biochemical Characterization of a Methanol Dehydrogenase From Lysinibacillus xylanilyticus Open
Bioconversion of C1 chemicals such as methane and methanol into higher carbon-chain chemicals has been widely studied. Methanol oxidation catalyzed by methanol dehydrogenase (Mdh) is one of the key steps in methanol utilization in bacteria…
View article: <scp>CRISPR</scp> interference‐mediated gene regulation in <i>Pseudomonas putida</i> <scp>KT</scp> 2440
<span>CRISPR</span> interference‐mediated gene regulation in <i>Pseudomonas putida</i> <span>KT</span> 2440 Open
Summary Targeted gene regulation is indispensable for reprogramming a cellular network to modulate a microbial phenotype. Here, we adopted the type II CRISPR interference ( CRISPR i) system for simple and efficient regulation of target gen…
View article: Complete Genome Sequence of the Soil Bacterium <i>Pseudomonas kribbensis</i> Strain 46-2 <sup>T</sup>
Complete Genome Sequence of the Soil Bacterium <i>Pseudomonas kribbensis</i> Strain 46-2 <sup>T</sup> Open
Pseudomonas kribbensis is a novel species belonging to the Pseudomonas fluorescens intrageneric group of the genus Pseudomonas . Herein, we report the complete genome sequence of strain 46-2 T , isolated from garden soil in Daejeon, South …
View article: A synthetic microbial biosensor for high-throughput screening of lactam biocatalysts
A synthetic microbial biosensor for high-throughput screening of lactam biocatalysts Open
View article: Evolution of enzymes with new specificity by high-throughput screening using DmpR-based genetic circuits and multiple flow cytometry rounds
Evolution of enzymes with new specificity by high-throughput screening using DmpR-based genetic circuits and multiple flow cytometry rounds Open
View article: A novel psychrophilic alkaline phosphatase from the metagenome of tidal flat sediments
A novel psychrophilic alkaline phosphatase from the metagenome of tidal flat sediments Open