Eva Forsten
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Primary high-throughput screening of engineered phytases by online monitoring of the oxygen transfer rate of Komagataella phaffii Open
Background Recombinant phytase production has recently gained increased recognition in phosphate recycling from phytate contained in plant-based side and waste streams. Until now, new phytase variants are evaluated at the end of the expres…
Small-scale fed-batch cultivations of Vibrio natriegens: overcoming challenges for early process development Open
Vibrio natriegens is a fast-growing microbial workhorse with high potential for biotechnological applications. However, handling the bacterium in batch processes is challenging due to its high overflow metabolism and mixed acid formation u…
High‐sensitivity real‐time monitoring of <span>pH</span> and respiration activity unveils metabolic dynamics in shake flask cultures Open
Erlenmeyer shake flasks are widely used during the first steps of bioprocess development. Despite their broad application in academia and industry, shake flasks usually lack standardized and user‐friendly online monitoring techniques. In t…
Unraveling the impact of pH, sodium concentration, and medium osmolality on Vibrio natriegens in batch processes Open
Background Vibrio natriegens , a halophilic marine γ-proteobacterium, holds immense biotechnological potential due to its remarkably short generation time of under ten minutes. However, the highest growth rates have been primarily observed…
Phosphate limitation enhances malic acid production on nitrogen-rich molasses with Ustilago trichophora Open
Background An important step in replacing petrochemical products with sustainable, cost-effective alternatives is the use of feedstocks other than, e.g., pure glucose in the fermentative production of platform chemicals. Ustilaginaceae off…
Inoculum cell count influences separation efficiency and variance in Ames plate incorporation and Ames RAMOS test Open
The Ames test is one of the most applied tools in mutagenicity testing of chemicals ever since its introduction by Ames et al. in the 1970s. Its principle is based on histidine auxotrophic bacteria that regain prototrophy through reverse m…