Eva Mie Lang Spedtsberg
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View article: First-class – biosynthesis of 6-MSA and bostrycoidin type I polyketides in Yarrowia lipolytica
First-class – biosynthesis of 6-MSA and bostrycoidin type I polyketides in Yarrowia lipolytica Open
Fungal polyketides are a large group of secondary metabolites, valuable due to their diverse spectrum of pharmacological activities. Polyketide biosynthesis in filamentous fungi presents some challenges: small yield and low-purity titers. …
View article: Yeast recombinational cloning for heterologous biosynthesis of polyketides: a molecular microbiology laboratory module for undergraduate students
Yeast recombinational cloning for heterologous biosynthesis of polyketides: a molecular microbiology laboratory module for undergraduate students Open
Recombinant plasmids are essential tools in molecular biotechnology, and reliable plasmid assembly methods have, therefore, become a prerequisite for the successful cloning and transfer of genes. Among the multitude of available plasmid as…
View article: Speed dating for enzymes! Finding the perfect phosphopantetheinyl transferase partner for your polyketide synthase
Speed dating for enzymes! Finding the perfect phosphopantetheinyl transferase partner for your polyketide synthase Open
The biosynthetic pathways for the fungal polyketides bikaverin and bostrycoidin, from Fusarium verticillioides and Fusarium solani respectively, were reconstructed and heterologously expressed in S. cerevisiae alongside seven different pho…
View article: Heterologous Expression of the Core Genes in the Complex Fusarubin Gene Cluster of Fusarium Solani
Heterologous Expression of the Core Genes in the Complex Fusarubin Gene Cluster of Fusarium Solani Open
Through stepwise recreation of the biosynthetic gene cluster containing PKS3 from Fusarium solani, it was possible to produce the core scaffold compound of bostrycoidin, a red aza-anthraquinone pigment in Saccharomyces cerevisiae. This was…