Eva Žerovnik
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View article: Molecular and cellular processes underlying Unverricht-Lundborg disease—prospects for early interventions and a cure
Molecular and cellular processes underlying Unverricht-Lundborg disease—prospects for early interventions and a cure Open
A short overview of the main features of progressive myoclonus epilepsies (PMEs), such as Lafora disease (LD), neuronal ceroid lipofuscinoses (NCLs), and myoclonus epilepsy with ragged-red fibers (MERRF) is given. The stress of this review…
View article: Editorial: Women in molecular neuroscience
Editorial: Women in molecular neuroscience Open
EDITORIAL article Front. Mol. Neurosci., 14 May 2024Sec. Brain Disease Mechanisms Volume 17 - 2024 | https://doi.org/10.3389/fnmol.2024.1422589
View article: Correction: Jahić Mujkić et al. Synergy of the Inhibitory Action of Polyphenols Plus Vitamin C on Amyloid Fibril Formation: Case Study of Human Stefin B. Antioxidants 2021, 10, 1471
Correction: Jahić Mujkić et al. Synergy of the Inhibitory Action of Polyphenols Plus Vitamin C on Amyloid Fibril Formation: Case Study of Human Stefin B. Antioxidants 2021, 10, 1471 Open
In the original publication [...]
View article: Protein Condensates and Protein Aggregates: In Vitro, in the Cell, and In Silico
Protein Condensates and Protein Aggregates: In Vitro, in the Cell, and In Silico Open
Similar to other polypeptides and electrolytes, proteins undergo phase transitions, obeying physicochemical laws. They can undergo liquid-to-gel and liquid-to-liquid phase transitions. Intrinsically disordered proteins are particularly sus…
View article: Amyloid Formation of Stefin B Protein Studied by Infrared Spectroscopy
Amyloid Formation of Stefin B Protein Studied by Infrared Spectroscopy Open
Background: Stefin B, an established model protein for studying the stability and mechanism of protein folding, was used for monitoring protein aggregation and formation of amyloid structure by infrared spectroscopy. Methods: The analyses …
View article: Amyloid Fibrils of Stefin B Show Anisotropic Properties
Amyloid Fibrils of Stefin B Show Anisotropic Properties Open
Human stefin B, a member of the cystatin family of cysteine protease inhibitors, tends to form amyloid fibrils under relatively mild conditions, which is why it is used as a model protein to study amyloid fibrillation. Here, we show for th…
View article: Human stefin B: from its structure, folding, and aggregation to its function in health and disease
Human stefin B: from its structure, folding, and aggregation to its function in health and disease Open
Mutations in the gene for human stefin B (cystatin B) cause progressive myoclonic epilepsy type 1 (EPM1), a neurodegenerative disorder. The most common change is dodecamer repeats in the promoter region of the gene, though missense and fra…
View article: Synergy of the Inhibitory Action of Polyphenols Plus Vitamin C on Amyloid Fibril Formation: Case Study of Human Stefin B
Synergy of the Inhibitory Action of Polyphenols Plus Vitamin C on Amyloid Fibril Formation: Case Study of Human Stefin B Open
In order to study how polyphenols and vitamin C (vitC) together affect protein aggregation to amyloid fibrils, we performed similar in vitro studies as before using stefin B as a model and a potentially amyloid-forming protein (it aggregat…
View article: Viroporins vs. Other Pore-Forming Proteins: What Lessons Can We Take?
Viroporins vs. Other Pore-Forming Proteins: What Lessons Can We Take? Open
Pore-forming proteins (PFPs) exist in virtually all domains of life, and by disrupting cellular membranes, depending on the pore size, they cause ion dis-balance, small substances, or even protein efflux/influx, influencing cell’s signalin…
View article: Prediction of Transmembrane Regions, Cholesterol, and Ganglioside Binding Sites in Amyloid-Forming Proteins Indicate Potential for Amyloid Pore Formation
Prediction of Transmembrane Regions, Cholesterol, and Ganglioside Binding Sites in Amyloid-Forming Proteins Indicate Potential for Amyloid Pore Formation Open
Besides amyloid fibrils, amyloid pores (APs) represent another mechanism of amyloid induced toxicity. Since hypothesis put forward by Arispe and collegues in 1993 that amyloid-beta makes ion-conducting channels and that Alzheimer's disease…
View article: On Similarities and Differences of HIV and SARS Cov-2; Open Questions Relevant for COVID-19 Disease
On Similarities and Differences of HIV and SARS Cov-2; Open Questions Relevant for COVID-19 Disease Open
This paper is written from an educated bio-scientist' perspective who follows studies about COVID-19 in order to better understand some relevant questions about the disease and vaccination. I first pose myself a question about similarities…
View article: Special Issue: “Inflammation, Oxidative Stress and Protein Aggregation; Any Links?”
Special Issue: “Inflammation, Oxidative Stress and Protein Aggregation; Any Links?” Open
Inflammation is a complex immune response that enables survival during infection and maintains tissue homeostasis [...]
View article: Antioxidants From the Class of Polyphenols, NAC and Vitamin C Act Differently on Amyloid Fibril Formation by Two Human Cystatins: Stefin B and Cystatin C.
Antioxidants From the Class of Polyphenols, NAC and Vitamin C Act Differently on Amyloid Fibril Formation by Two Human Cystatins: Stefin B and Cystatin C. Open
We compare the effect on amyloid fibril formation by two homologous proteins from the family of cystatins, human stefin B (stB) and cystatin C (cysC) in presence of 3 polyphenols: curcumin, resveratrol and quercetin and 2 non-phenolic anti…
View article: The effect of three polyphenols and some other antioxidant substances on amyloid fibril formation by Human cystatin C
The effect of three polyphenols and some other antioxidant substances on amyloid fibril formation by Human cystatin C Open
Human cystatin C (CysC) is an amyloid forming protein involved in the hereditary cerebral amyloid angiopathy (HCCAA) that affects arteries in the brain and the peripheral nervous system. In this study we measured the influence of several s…
View article: Assessing Autophagy in Archived Tissue or How to Capture Autophagic Flux from a Tissue Snapshot
Assessing Autophagy in Archived Tissue or How to Capture Autophagic Flux from a Tissue Snapshot Open
Autophagy is a highly conserved degradation mechanism that is essential for maintaining cellular homeostasis. In human disease, autophagy pathways are frequently deregulated and there is immense interest in targeting autophagy for therapeu…
View article: Prolines Affect the Nucleation Phase of Amyloid Fibrillation Reaction; Mutational Analysis of Human Stefin B
Prolines Affect the Nucleation Phase of Amyloid Fibrillation Reaction; Mutational Analysis of Human Stefin B Open
Proline residues play a prominent role in protein folding and aggregation. We investigated the influence of single prolines and their combination on oligomerization and the amyloid fibrillation reaction of human stefin B (stB). The proline…
View article: Possible Mechanisms by which Stefin B could Regulate Proteostasis and Oxidative Stress
Possible Mechanisms by which Stefin B could Regulate Proteostasis and Oxidative Stress Open
Human stefin B is a protease inhibitor from the family of cystatins. It was reported that it forms oligomers in cells. We have shown that it has a role in cell’s response to misfolded proteins. We also have shown that its oligomers bind am…
View article: From Oxidative Stress Damage to Pathways, Networks, and Autophagy via MicroRNAs
From Oxidative Stress Damage to Pathways, Networks, and Autophagy via MicroRNAs Open
Oxidative stress can alter the expression level of many microRNAs (miRNAs), but how these changes are integrated and related to oxidative stress responses is poorly understood. In this article, we addressed this question by using in silico…
View article: Inhibition of Protein Aggregation by Several Antioxidants
Inhibition of Protein Aggregation by Several Antioxidants Open
Amyloid fibril formation is a shared property of all proteins; therefore, model proteins can be used to study this process. We measured protein aggregation of the model amyloid‐forming protein stefin B in the presence and absence of severa…
View article: Proline Residues as Switches in Conformational Changes Leading to Amyloid Fibril Formation
Proline Residues as Switches in Conformational Changes Leading to Amyloid Fibril Formation Open
Here we discuss studies of the structure, folding, oligomerization and amyloid fibril formation of several proline mutants of human stefin B, which is a protein inhibitor of lysosomal cysteine cathepsins and a member of the cystatin family…
View article: Recent Developments in Treating Alzheimer’s Disease
Recent Developments in Treating Alzheimer’s Disease Open
Early diagnosis and efficient treatment for sporadic Alzheimer’s disease (AD) are urgently needed as the condition becomes an increasing burden within aging societies. We collected recent data on the progress towards effective treatments o…