Evan Appleton
YOU?
Author Swipe
View article: Simulating a <i>for-loop</i> in the human genome: Design and evaluation of recombinase genetic programs that count to three
Simulating a <i>for-loop</i> in the human genome: Design and evaluation of recombinase genetic programs that count to three Open
Summary Pluripotent cells specialize into numerous cell types by receiving external signals, making fate decisions, and executing differentiation functions – a paradigm similar to computer algorithms. While advances in biosensor design hav…
View article: Machine-guided cell-fate engineering
Machine-guided cell-fate engineering Open
The creation of induced pluripotent stem cells (iPSCs) has enabled scientists to explore the function, mechanisms, and differentiation processes of many types of cells. One of the fastest and most efficient approaches is transcription fact…
View article: Algorithms for Autonomous Formation of Multicellular Shapes from Single Cells
Algorithms for Autonomous Formation of Multicellular Shapes from Single Cells Open
Multicellular organisms originate from a single cell, ultimately giving rise to mature organisms of heterogeneous cell type composition in complex structures. Recent work in the areas of stem cell biology and tissue engineering has laid ma…
View article: Derivation of elephant induced pluripotent stem cells
Derivation of elephant induced pluripotent stem cells Open
The crisis of biodiversity loss in the anthropogenic era requires new tools for studying non-model organisms. Elephants, for example, are both an endangered species and excellent models studying complex phenotypes like size, social behavio…
View article: Machine-guided cell-fate engineering
Machine-guided cell-fate engineering Open
A bstract The creation of induced pluripotent stem cells (iPSCs) has enabled scientists to explore the derivation of many types of cells. While there are diverse general approaches for cell-fate engineering, one of the fastest and most eff…
View article: Iterative transcription factor screening enables rapid generation of microglia-like cells from human iPSC
Iterative transcription factor screening enables rapid generation of microglia-like cells from human iPSC Open
The ability to differentiate stem cells into human cell types is essential to define basic mechanisms and therapeutics, especially for cell types not routinely accessible by biopsies. But while engineered expression of transcription factor…
View article: helixCAM: A Platform for Programmable Cellular Assembly in Bacteria and Human Cells
helixCAM: A Platform for Programmable Cellular Assembly in Bacteria and Human Cells Open
Summary Interactions between cells are indispensable for creating structure and signaling. The ability to direct precise cell-cell interactions would be powerful for engineering tissues, understanding signaling pathways, and directing immu…
View article: Cell therapy strategies for COVID-19: Current approaches and potential applications
Cell therapy strategies for COVID-19: Current approaches and potential applications Open
Cell therapy has strong promise for treating COVID-19 survivors due to customizability, targetability, and regenerative function.
View article: Synthetic auxotrophy remains stable after continuous evolution and in coculture with mammalian cells
Synthetic auxotrophy remains stable after continuous evolution and in coculture with mammalian cells Open
A synthetic auxotroph does not escape from biocontainment after evolution, demonstrating that life does not always find a way.
View article: Technical Aspects of Biosecurity: Screening Guidance, Attribution, and Traceability
Technical Aspects of Biosecurity: Screening Guidance, Attribution, and Traceability Open
Biosecurity is a multi-disciplinary topic that covers areas of policy, public health, economics, and science. This chapter focuses on the technical scientific aspects of the current international biosecurity framework. We discuss these tec…
View article: Synthetic auxotrophy remains stable after continuous evolution and in co-culture with mammalian cells
Synthetic auxotrophy remains stable after continuous evolution and in co-culture with mammalian cells Open
Understanding the evolutionary stability and possible context-dependence of biological containment techniques is critical as engineered microbes are increasingly under consideration for applications beyond biomanufacturing. While batch cul…
View article: Algorithms for the selection of fluorescent reporters
Algorithms for the selection of fluorescent reporters Open
Molecular biologists rely on the use of fluorescent probes to take measurements of their model systems. These fluorophores fall into various classes (e.g. fluorescent dyes, fluorescent proteins, etc.), but they all share some general prope…
View article: Genetic design automation for autonomous formation of multicellular shapes from a single cell progenitor
Genetic design automation for autonomous formation of multicellular shapes from a single cell progenitor Open
A bstract Multi-cellular organisms originate from a single cell, ultimately giving rise to mature organisms of heterogeneous cell type composition in complex structures. Recent work in the areas of stem cell biology and tissue engineering …
View article: Design Automation in Synthetic Biology
Design Automation in Synthetic Biology Open
Design automation refers to a category of software tools for designing systems that work together in a workflow for designing, building, testing, and analyzing systems with a target behavior. In synthetic biology, these tools are called bi…
View article: A design-build-test-learn tool for synthetic biology
A design-build-test-learn tool for synthetic biology Open
Modern synthetic gene regulatory networks emerge from iterative design-build-test cycles that encompass the decisions and actions necessary to design, build, and test target genetic systems. Historically, such cycles have been performed ma…
View article: BBF RFC 94: Type IIS Assembly for Bacterial Transcriptional Units: A Standardized Assembly Method for Building Bacterial Transcriptional Units Using the Type IIS Restriction Enzymes BsaI and BbsI
BBF RFC 94: Type IIS Assembly for Bacterial Transcriptional Units: A Standardized Assembly Method for Building Bacterial Transcriptional Units Using the Type IIS Restriction Enzymes BsaI and BbsI Open
This RFC94 describes an assembly standard based on the Type IIS restriction enzymes BsaI and BbsI (also called BpiI). This assembly standard is based upon the Modular Cloning (MoClo) assembly strategy, which was introduced in 2011 by Weber…