Evan M. Green
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View article: Structural basis for HCMV Pentamer receptor recognition and antibody neutralization
Structural basis for HCMV Pentamer receptor recognition and antibody neutralization Open
Human cytomegalovirus (HCMV) represents the viral leading cause of congenital birth defects and uses the gH/gL/UL128-130-131A complex (Pentamer) to enter different cell types, including epithelial and endothelial cells. Upon infection, Pen…
View article: Identification of recombinant Fabs for structural and functional characterization of HIV-host factor complexes
Identification of recombinant Fabs for structural and functional characterization of HIV-host factor complexes Open
Viral infection and pathogenesis is mediated by host protein—viral protein complexes that are important targets for therapeutic intervention as they are potentially less prone to development of drug resistance. We have identified human, re…
View article: Antibody-Drug Conjugates Targeting the Urokinase Receptor (uPAR) as a Possible Treatment of Aggressive Breast Cancer
Antibody-Drug Conjugates Targeting the Urokinase Receptor (uPAR) as a Possible Treatment of Aggressive Breast Cancer Open
A promising molecular target for aggressive cancers is the urokinase receptor (uPAR). A fully human, recombinant antibody that binds uPAR to form a stable complex that blocks uPA-uPAR interactions (2G10) and is internalized primarily throu…
View article: Why recombinant antibodies — benefits and applications
Why recombinant antibodies — benefits and applications Open
View article: Structural basis for activation of voltage sensor domains in an ion channel TPC1
Structural basis for activation of voltage sensor domains in an ion channel TPC1 Open
Significance This paper addresses how the structures of voltage-sensing domains within voltage-gated ion channels respond to physiological changes in electric potential across membranes. We report three new structures of TPC1 by cryo-EM an…
View article: The structural basis for activation of voltage sensor domains in an ion channel TPC1
The structural basis for activation of voltage sensor domains in an ion channel TPC1 Open
Voltage sensing domains (VSDs) couple changes in transmembrane electrical potential to conformational changes that regulate ion conductance through a central channel. Positively charged amino acids inside each sensor cooperatively respond …
View article: Extending chemical perturbations of the ubiquitin fitness landscape in a classroom setting reveals new constraints on sequence tolerance
Extending chemical perturbations of the ubiquitin fitness landscape in a classroom setting reveals new constraints on sequence tolerance Open
Although the primary protein sequence of ubiquitin (Ub) is extremely stable over evolutionary time, it is highly tolerant to mutation during selection experiments performed in the laboratory. We have proposed that this discrepancy results …
View article: Thermostabilization, Expression, Purification, and Crystallization of the Human Serotonin Transporter Bound to <em>S</em>-citalopram
Thermostabilization, Expression, Purification, and Crystallization of the Human Serotonin Transporter Bound to <em>S</em>-citalopram Open
The serotonin transporter is a sodium and chloride-coupled transporter that "pumps" extracellular serotonin into cells. S-citalopram is a drug used to treat depression and anxiety by binding to the serotonin transporter with high-affinity,…
View article: Thermostabilization, Expression, Purification, and Crystallization of the Human Serotonin Transporter Bound to <em>S</em>-citalopram
Thermostabilization, Expression, Purification, and Crystallization of the Human Serotonin Transporter Bound to <em>S</em>-citalopram Open
The serotonin transporter is a sodium and chloride-coupled transporter that "pumps" extracellular serotonin into cells. S-citalopram is a drug used to treat depression and anxiety by binding to the serotonin transporter with high-affinity,…
View article: Determination of ubiquitin fitness landscapes under different chemical stresses in a classroom setting
Determination of ubiquitin fitness landscapes under different chemical stresses in a classroom setting Open
Ubiquitin is essential for eukaryotic life and varies in only 3 amino acid positions between yeast and humans. However, recent deep sequencing studies indicate that ubiquitin is highly tolerant to single mutations. We hypothesized that thi…
View article: Author response: Determination of ubiquitin fitness landscapes under different chemical stresses in a classroom setting
Author response: Determination of ubiquitin fitness landscapes under different chemical stresses in a classroom setting Open
Article Figures and data Abstract eLife digest Introduction Results Discussion Materials and methods Data availability References Decision letter Author response Article and author information Metrics Abstract Ubiquitin is essential for eu…
View article: X-ray structures and mechanism of the human serotonin transporter
X-ray structures and mechanism of the human serotonin transporter Open
View article: Thermostabilization of the Human Serotonin Transporter in an Antidepressant-Bound Conformation
Thermostabilization of the Human Serotonin Transporter in an Antidepressant-Bound Conformation Open
Serotonin is a ubiquitous chemical transmitter with particularly important roles in the gastrointestinal, cardiovascular and central nervous systems. Modulation of serotonergic signaling occurs, in part, by uptake of the transmitter by the…
View article: Determination of Ubiquitin Fitness Landscapes Under Different Chemical Stresses in a Classroom Setting
Determination of Ubiquitin Fitness Landscapes Under Different Chemical Stresses in a Classroom Setting Open
Ubiquitination is an essential post-translational regulatory process that can control protein stability, localization, and activity. Ubiquitin is essential for eukaryotic life and is highly conserved, varying in only 3 amino acid positions…