Evan P. Perillo
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View article: High-plex Multiomic Analysis in FFPE at Subcellular Level by Spatial Molecular Imaging
High-plex Multiomic Analysis in FFPE at Subcellular Level by Spatial Molecular Imaging Open
The Spatial Molecular Imaging platform (CosMx TM SMI, NanoString Technologies, Seattle, WA) utilizes high-plex in-situ imaging chemistry for both RNA and protein detection. This automated instrument provides 1000’s of plex, at high sensiti…
Diamond Raman Laser and Yb Fiber Amplifier for <i>In Vivo</i> Multiphoton Fluorescence Microscopy Open
Here we introduce a fiber amplifier and a diamond Raman laser that output high powers (6.5 W, 1.3 W) at valuable wavelengths (1060 nm, 1250 nm) for multiphoton excitation of red-shifted fluorophores. These custom excitation sources are bot…
Three-Dimensional Two-Color Dual-Particle Tracking Microscope for Monitoring DNA Conformational Changes and Nanoparticle Landings on Live Cells Open
Here, we present a three-dimensional two-color dual-particle tracking (3D-2C-DPT) technique that can simultaneously localize two spectrally distinct targets in three dimensions with a time resolution down to 5 ms. The dual-targets can be t…
View article: Front Matter: Volume 11244
Front Matter: Volume 11244 Open
A unique citation identifier (CID) number is assigned to each article at the time of
Spatial EGFR Dynamics and Metastatic Phenotypes Modulated by Upregulated EphB2 and Src Pathways in Advanced Prostate Cancer Open
Advanced prostate cancer is a very heterogeneous disease reflecting in diverse regulations of oncogenic signaling pathways. Aberrant spatial dynamics of epidermal growth factor receptor (EGFR) promote their dimerization and clustering, lea…
Abstract 173: Upregulated EPHB2 and SRC pathways modulate spatial EGFR dynamics and malignant phenotypes and predict poor prognosis in prostate cancer Open
Dysregulated dynamics and trafficking of receptor tyrosine kinases (RTK) have been linked to oncogenesis and metastasis. The mechanisms for disfunction of RTK dynamics are emerging and still not clear. Using single-particle tracking (SPT) …
View article: Polymer dots enable deep in vivo multiphoton fluorescence imaging of microvasculature
Polymer dots enable deep in vivo multiphoton fluorescence imaging of microvasculature Open
Deep in vivo imaging of vasculature requires small, bright, and photostable fluorophores suitable for multiphoton microscopy (MPM). Although semiconducting polymer dots (pdots) are an emerging class of highly fluorescent contrast agents wi…
View article: <i>In vivo</i> multiphoton fluorescence imaging with polymer dots
<i>In vivo</i> multiphoton fluorescence imaging with polymer dots Open
Deep in vivo imaging of vasculature requires small, bright, and photostable fluorophores suitable for multiphoton microscopy (MPM). Although semiconducting polymer dots (pdots) are an emerging class of highly fluorescent contrast agents wi…
View article: Plasmonic Nanostructures: Controlling Plasmon‐Enhanced Fluorescence via Intersystem Crossing in Photoswitchable Molecules (Small 38/2017)
Plasmonic Nanostructures: Controlling Plasmon‐Enhanced Fluorescence via Intersystem Crossing in Photoswitchable Molecules (Small 38/2017) Open
In article number 1701763, Yuebing Zheng and co-workers report how intersystem crossing (ISC) controls plasmon-enhanced fluorescence in hybrid systems of plasmonic nanostructures and spiropyran molecules. They further demonstrate An all-op…
Controlling Plasmon‐Enhanced Fluorescence via Intersystem Crossing in Photoswitchable Molecules Open
By harnessing photoswitchable intersystem crossing (ISC) in spiropyran (SP) molecules, active control of plasmon‐enhanced fluorescence in the hybrid systems of SP molecules and plasmonic nanostructures is achieved. Specifically, SP‐derived…
In vivo multiphoton imaging of a diverse array of fluorophores to investigate deep neurovascular structure Open
We perform high-resolution, non-invasive, in vivo deep-tissue imaging of the mouse neocortex using multiphoton microscopy with a high repetition rate optical parametric amplifier laser source tunable between λ=1,100 and 1,400 nm. By combin…
High-Resolution Bubble Printing of Quantum Dots Open
Semiconductor quantum dots (QDs) feature excellent properties, such as high quantum efficiency, tunable emission frequency, and good fluorescence stability. Incorporation of QDs into new devices relies upon high-resolution and high-through…
Patterning and fluorescence tuning of quantum dots with haptic-interfaced bubble printing Open
Sub-micron resolution patterning and fluorescence tuning of CdSe/CdS quantum dots using haptic-interfaced bubble printing (HIBP) is reported.
Nonlinear microscopy methods for imaging and particle tracking in thick biological specimen Open
Optical microscopy techniques such as single-particle tracking and high-resolution (<500 nm) imaging are critical tools for the advancement of biological research. However most high-resolution optical techniques utilize a camera-based or c…
View article: Molecular-Fluorescence Enhancement via Blue-Shifted Plasmon-Induced Resonance Energy Transfer
Molecular-Fluorescence Enhancement via Blue-Shifted Plasmon-Induced Resonance Energy Transfer Open
We report molecular-fluorescence enhancement via the blue-shifted plasmon-induced resonance energy transfer (PIRET) from single Au nanorods (AuNRs) to merocyanine (MC) dye molecules. The blue-shifted PIRET occurs when there is a proper spe…
Single-Molecule Tracking and Its Application in Biomolecular Binding Detection Open
In the past two decades significant advances have been made in single-molecule detection, which enables the direct observation of single biomolecules at work in real time and under physiological conditions. In particular, the development o…
Deep in vivo two-photon microscopy with a low cost custom built mode-locked 1060 nm fiber laser Open
Here we demonstrate that a mode-locked ytterbium fiber laser for two-photon fluorescence microscopy can be built for $13,000. The laser emits at a wavelength of 1060 nm with a usable average power of 1 W at a repetition rate of 40 MHz and …