Evgeny Onischenko
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View article: Vsb1, Ypq1 and Ypq2 control dynamic cationic amino acid storage in the yeast vacuole
Vsb1, Ypq1 and Ypq2 control dynamic cationic amino acid storage in the yeast vacuole Open
Although the yeast vacuole plays a crucial role in storing and mobilizing cationic amino acids (CAA), CAA transport at the vacuolar membrane remains poorly understood. Here, by combining analysis of CAA pools, uptake and permeabilization a…
View article: An amphipathic helix in Brl1 is required for nuclear pore complex biogenesis in S. cerevisiae
An amphipathic helix in Brl1 is required for nuclear pore complex biogenesis in S. cerevisiae Open
The nuclear pore complex (NPC) is the central portal for macromolecular exchange between the nucleus and cytoplasm. In all eukaryotes, NPCs assemble into an intact nuclear envelope (NE) during interphase, but the process of NPC biogenesis …
View article: The Nuclear Pore Complex: Birth, Life, and Death of a Cellular Behemoth
The Nuclear Pore Complex: Birth, Life, and Death of a Cellular Behemoth Open
Nuclear pore complexes (NPCs) are the only transport channels that cross the nuclear envelope. Constructed from ~500–1000 nucleoporin proteins each, they are among the largest macromolecular assemblies in eukaryotic cells. Thanks to advanc…
View article: An amphipathic helix in Brl1 is required for membrane fusion during nuclear pore complex biogenesis in <i>S. cerevisiae</i>
An amphipathic helix in Brl1 is required for membrane fusion during nuclear pore complex biogenesis in <i>S. cerevisiae</i> Open
The nuclear pore complex (NPC) is the central portal for macromolecular exchange between the nucleus and cytoplasm. In all eukaryotes, NPCs assemble into an intact nuclear envelope (NE) during interphase, but the process of NPC biogenesis …
View article: Code for Maturation kinetics of a multiprotein complex revealed by metabolic labeling
Code for Maturation kinetics of a multiprotein complex revealed by metabolic labeling Open
A project for fitting a (binary) assembly tree to dynamic labeling data from AP-MS proteomics. This code was used in the work published as "Maturation kinetics of a multiprotein complex revealed by metabolic labeling" in Onischenko et al. …
View article: Mapping the native organization of the yeast nuclear pore complex using nuclear radial intensity measurements
Mapping the native organization of the yeast nuclear pore complex using nuclear radial intensity measurements Open
Significance The nuclear pore complex (NPC) is a huge protein assembly that selectively transports macromolecules across the nuclear envelope (NE) of eukaryotes. Determining the precise organization of that machinery has been a long-standi…
View article: Stoichiometry and compositional plasticity of the yeast nuclear pore complex revealed by quantitative fluorescence microscopy
Stoichiometry and compositional plasticity of the yeast nuclear pore complex revealed by quantitative fluorescence microscopy Open
The nuclear pore complex (NPC) is an 8-fold symmetrical channel providing selective transport of biomolecules across the nuclear envelope. Each NPC consists of ~30 different nuclear pore proteins (Nups) all present in multiple copies per N…