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View article: Beyond synthetic lethality in large-scale metabolic and regulatory network models via genetic minimal intervention sets
Beyond synthetic lethality in large-scale metabolic and regulatory network models via genetic minimal intervention sets Open
Motivation The integration of genome-scale metabolic and regulatory networks has received significant interest in the field of Cancer Systems Biology. However, the identification of lethal genetic interventions in these integrated models r…
View article: A functional CAR-T cell atlas to unravel regulatory mechanisms of CAR-T therapies
A functional CAR-T cell atlas to unravel regulatory mechanisms of CAR-T therapies Open
Introduction: Over the past decade, CAR-T therapies have emerged as a groundbreaking treatment for cancer, showing remarkable efficacy in certain hematologic malignancies. However, these therapies still face important limitations, includin…
View article: Integrated single-cell and spatial analysis identifies T cell exhaustion and adhesion signatures in early follicular lymphoma transformation
Integrated single-cell and spatial analysis identifies T cell exhaustion and adhesion signatures in early follicular lymphoma transformation Open
INTRODUCTION. Germinal center (GC) lymphomas, including follicular lymphoma (FL) and diffuse large B cell lymphoma (DLBCL), arise from B cells at distinct GC stages and display molecular and clinical heterogeneity. FL transformation is ass…
View article: Systematic production of human kidney organoids for transplantation in porcine kidneys during ex vivo machine perfusion
Systematic production of human kidney organoids for transplantation in porcine kidneys during ex vivo machine perfusion Open
Organoids derived from human pluripotent stem (hPS) cells hold promise for therapeutic purposes. However, technological advances to overcome their massive production while ensuring differentiation fidelity are still lacking. Here we report…
View article: An open CAR-T single-cell atlas to enable in-depth characterization and rational engineering of CAR-T products
An open CAR-T single-cell atlas to enable in-depth characterization and rational engineering of CAR-T products Open
BRIEF ABSTRACT We built a CAR-T cell functional atlas from over one million cells across 13 studies, integrating data from patients and healthy donors. The atlas captures 11 phenotypes, links infusion product composition with clinical resp…
View article: Uncovering the regulatory landscape of early human B cell lymphopoiesis and its implications in the pathogenesis of B-ALL
Uncovering the regulatory landscape of early human B cell lymphopoiesis and its implications in the pathogenesis of B-ALL Open
Dysregulation of early B cell lymphopoiesis—the process guiding cellular immunity development—can lead to malignancy, making it crucial to understand its regulatory mechanisms. We generated a multiomics resource comprising paired chromatin…
View article: PROGNOSIS - PROGNOSTIC FACTORS OF OUTCOME AND RISK ASSESSMENT
PROGNOSIS - PROGNOSTIC FACTORS OF OUTCOME AND RISK ASSESSMENT Open
View article: Call for preserving specialized knowledge and contributions of the CAT to advancing ATMPs in Europe
Call for preserving specialized knowledge and contributions of the CAT to advancing ATMPs in Europe Open
With the proposal for major reform of the pharmaceutical legislation (Regulation (EU) 2023/0131, Directive (EU) 2023/0132) in April 2023, the foundations of Europe's regulatory architecture are being reshaped. The revised legislation propo…
View article: Melt electrowritten 3D human cardiac engineered tissues reproduce the enhanced sensitivity of fibrotic patients to doxorubicin
Melt electrowritten 3D human cardiac engineered tissues reproduce the enhanced sensitivity of fibrotic patients to doxorubicin Open
Introduction Cardiotoxicity is a serious and frequent side effect of anthracycline cancer therapy, prompting the cardio-oncologic follow up of patients. However, mechanisms are poorly understood. Moreover, cardiac patients demonstrate an e…
View article: Histone methyl-transferase G9a inhibition boosts the efficacy of immune checkpoint inhibitors in experimental hepatocellular carcinoma
Histone methyl-transferase G9a inhibition boosts the efficacy of immune checkpoint inhibitors in experimental hepatocellular carcinoma Open
Background and Aims Immune checkpoint inhibitors (ICI) have revolutionized cancer therapy. Yet, their efficacy in hepatocellular carcinoma (HCC) remains limited, partly due to tumor-intrinsic mechanisms of immune evasion. This study focuse…
View article: Uncovering cell type-specific phenotypes using a novel human in vitro model of transthyretin amyloid cardiomyopathy
Uncovering cell type-specific phenotypes using a novel human in vitro model of transthyretin amyloid cardiomyopathy Open
Our model shows that WT and variant TTR fibrils lead to cell type-specific phenotypes, providing novel insights into the underlying cellular disease mechanisms of ATTR-CM, thereby facilitating the identification of novel therapeutic target…
View article: 179 | GENOMIC AND TRANSCRIPTIONAL SINGLE‐CELL HETEROGENEITY IN GERMINAL‐CENTER LYMPHOMAS: INSIGHTS INTO FOLLICULAR LYMPHOMA TRANSFORMATION
179 | GENOMIC AND TRANSCRIPTIONAL SINGLE‐CELL HETEROGENEITY IN GERMINAL‐CENTER LYMPHOMAS: INSIGHTS INTO FOLLICULAR LYMPHOMA TRANSFORMATION Open
View article: Airway basal stem cells are necessary for the maintenance of functional intraepithelial airway macrophages
Airway basal stem cells are necessary for the maintenance of functional intraepithelial airway macrophages Open
View article: Aptameroak Mieloma Anizkoitzaren aurkako RNA-n oinarritutako terapien garapenerako
Aptameroak Mieloma Anizkoitzaren aurkako RNA-n oinarritutako terapien garapenerako Open
Mieloma Anizkoitza hezur-muineko zelula plasmatikoen minbizia da, hein handi batean oraindik sendagaitz dirauena. Berriki, RNA ez-kodetzaileak (lncRNA) gaixotasun honen tratamendurako itu terapeutiko bezala proposatu dira. Hala ere, moleku…
View article: Co-targeting of epigenetic regulators and BCL-XL improves efficacy of immune checkpoint blockade therapy in multiple solid tumors
Co-targeting of epigenetic regulators and BCL-XL improves efficacy of immune checkpoint blockade therapy in multiple solid tumors Open
View article: From Sanger to Oxford Nanopore MinION Technology: The Impact of Third-Generation Sequencing on Genetic Hematological Diagnosis
From Sanger to Oxford Nanopore MinION Technology: The Impact of Third-Generation Sequencing on Genetic Hematological Diagnosis Open
Background: Sanger sequencing remains the gold standard for characterizing genetic variants in short DNA fragments (<700 bp). However, the increasing demand for short TATs and high sensitivities in variant detection, particularly in oncohe…
View article: Biologically‐Inspired Melt Electrowriting for the Generation of Highly Biomimetic Functional Myocardium
Biologically‐Inspired Melt Electrowriting for the Generation of Highly Biomimetic Functional Myocardium Open
In the heart, the specific 3D structure of myocardial layers produces an efficient ejection of blood. When myocardial infarction strikes, this architecture is disrupted, adding a disarranged contraction to the decreased availability of pum…
View article: Single-Cell Multiomics Reveals Regulatory Mechanisms of CAR T Cell Persistence and Dysfunction in Multiple Myeloma
Single-Cell Multiomics Reveals Regulatory Mechanisms of CAR T Cell Persistence and Dysfunction in Multiple Myeloma Open
Understanding the mechanisms that drive chimeric antigen receptor (CAR) T cell function and persistence in multiple myeloma (MM) remains a critical challenge for improving therapeutic outcomes. In this study, we applied single-cell multiom…
View article: Chaperone-mediated autophagy sustains pericyte stemness necessary for brain tissue homeostasis
Chaperone-mediated autophagy sustains pericyte stemness necessary for brain tissue homeostasis Open
we show that manipulation of CMA activity in host PCs could be a useful therapeutical approach in the context of brain inflammation, which might be extended to other diseases where the pericyte has a key role in response to inflammation.
View article: Implication of bone marrow adipose tissue in bone homeostasis during osteoarthritis
Implication of bone marrow adipose tissue in bone homeostasis during osteoarthritis Open
All these results support the emergence of BMAds as new cell partners in OA, opening new venues for therapeutic approaches.
View article: P-08.01 A combinatorial therapy for bladder cancer: epigenetic inhibitor and CAR-NK cells
P-08.01 A combinatorial therapy for bladder cancer: epigenetic inhibitor and CAR-NK cells Open
View article: Human growth plates house resting zone sub-populations with features of quiescent stem cells
Human growth plates house resting zone sub-populations with features of quiescent stem cells Open
Incomplete mapping of gene expression within human (epiphyseal) growth plates contributes to the challenges of diagnosing and treating patients with skeletal growth disorders. To address this issue, we applied spatially resolved transcript…
View article: Continuous map of early hematopoietic stem cell differentiation across human lifetime
Continuous map of early hematopoietic stem cell differentiation across human lifetime Open
View article: Discovery and preclinical development of a SdAb-based CAR-T technology for targeting CD33 in AML
Discovery and preclinical development of a SdAb-based CAR-T technology for targeting CD33 in AML Open
View article: Phosphatidylserine as a tumor target for CAR-T cell therapy
Phosphatidylserine as a tumor target for CAR-T cell therapy Open
Background Phosphatidylserine (PS) exposed on apoptotic cells promotes immune clearance of dead cells without inducing inflammation. Conversely, PS exposure on live tumor cells promotes an immunosuppressive tumor microenvironment that hind…
View article: Generation and GMP scale-up of human CAR-T cells using non-viral Sleeping Beauty transposons for B cell malignances
Generation and GMP scale-up of human CAR-T cells using non-viral Sleeping Beauty transposons for B cell malignances Open
Most CAR-T therapies rely on genetic T cell engineering with integrating viral vectors that, although effective, are associated with prohibitive costs. Here we have generated TranspoCART19 cells, a fully functional 4-1BB second-generation …
View article: Effect of Hyaluronic Acid Compared to Platelet-Rich Plasma as Adjuvants to Bone Marrow Mesenchymal Stem Cell Treatment of Knee Osteoarthritis: Analysis from Two Clinical Trials
Effect of Hyaluronic Acid Compared to Platelet-Rich Plasma as Adjuvants to Bone Marrow Mesenchymal Stem Cell Treatment of Knee Osteoarthritis: Analysis from Two Clinical Trials Open
Background: Bone marrow mesenchymal stem cell (BM-MSC) therapy has emerged as a safe and feasible treatment option for patients with knee osteoarthritis (OA). However, the role of adjuvants remains unclear. Our aim was to evaluate the clin…
View article: Billion‐Scale Expansion of Functional hiPSC‐Derived Cardiomyocytes in Bioreactors Through Oxygen Control and Continuous Wnt Activation
Billion‐Scale Expansion of Functional hiPSC‐Derived Cardiomyocytes in Bioreactors Through Oxygen Control and Continuous Wnt Activation Open
Generation of upscaled quantities of human‐induced pluripotent stem cell‐derived cardiomyocytes (hiPSC‐CM), for therapeutic or testing applications, is both expensive and time‐consuming. Herein, a scalable bioprocess for hiPSC‐CM expansion…
View article: Targeting of the G9a, DNMT1 and UHRF1 epigenetic complex as an effective strategy against pancreatic ductal adenocarcinoma
Targeting of the G9a, DNMT1 and UHRF1 epigenetic complex as an effective strategy against pancreatic ductal adenocarcinoma Open
Background Pancreatic ductal adenocarcinoma (PDAC) is a highly aggressive cancer with limited treatment options and a poor prognosis. The critical role of epigenetic alterations such as changes in DNA methylation, histones modifications, a…
View article: Journal of Pharmacology and Experimental Therapeutics
Journal of Pharmacology and Experimental Therapeutics Open