2025-05-22
Ultrasensitive detection of circulating multiple myeloma cells by next-generation flow after immunomagnetic enrichment
2025-05-22 • Marta Lasa, Carmen González, Laura Notarfranchi, Anastasiia Zherniakova, Diego Alignani, Leire Burgos, Marı́a José Calasanz, Paula Rodríguez‐Otero,...
Abstract The continuous improvement in progression-free survival (PFS) of patients with multiple myeloma (MM) raises interest in evaluating peripheral residual disease (PRD) toward more frequent readouts of tumor kinetics while preserving quality of life. We present BloodFlow, a new method combining immunomagnetic enrichment of CD138+ circulating plasma cells in peripheral blood (PB) with next-generation flow (NGF), for the detection of PRD below the 2 × 10–6 NGF threshold. BloodFlow detected PRD in 55 of 644 PB s…