Roberto F. Machado
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View article: Caveolin‐1 Is a Central Mediator of Acute Lung Injury in an Animal Model of Sickle Cell Disease
Caveolin‐1 Is a Central Mediator of Acute Lung Injury in an Animal Model of Sickle Cell Disease Open
Acute chest syndrome in sickle cell disease (SCD) carries high morbidity and mortality, with up to 15% of patients requiring invasive mechanical ventilation. However, the rates and mechanisms of ventilator‐induced lung injury (VILI) in SCD…
View article: Pulmonary Hypertension Promotes Neuroinflammation and Neurodegeneration
Pulmonary Hypertension Promotes Neuroinflammation and Neurodegeneration Open
Introduction Pulmonary arterial hypertension (PAH) is associated with neurocognitive deficits and abnormal brain MRI. Little is known about the mechanisms underlying these clinical observations. TDP-43 is a proteinopathy associated with fr…
View article: Dicentrine and its N-oxide derivatives induces apoptotic and necrotic cell death in prostate cancer cell lines
Dicentrine and its N-oxide derivatives induces apoptotic and necrotic cell death in prostate cancer cell lines Open
As part of our continuous research for the discovery of anticancer natural products, the effects of alkaloid (6aS)-dicentrine (1) and its respective (6aS,6S)- (1a) and (6aS,6R)-(1b) N-oxides against two prostate cancer cell lines (PC3 and …
View article: Partial Wwox Loss of Function Increases Severity of Murine Sepsis and Neuroinflammation
Partial Wwox Loss of Function Increases Severity of Murine Sepsis and Neuroinflammation Open
Rationale WW domain-containing oxidoreductase ( WWOX ) is a gene associated implicated in both neurologic and inflammatory diseases and is susceptible to environmental stressors. We hypothesize partial loss of Wwox function will result in …
View article: Plasma Proteomics Identifies B2M as a Regulator of Pulmonary Hypertension in Heart Failure With Preserved Ejection Fraction
Plasma Proteomics Identifies B2M as a Regulator of Pulmonary Hypertension in Heart Failure With Preserved Ejection Fraction Open
BACKGROUND: Pulmonary hypertension (PH) represents an important phenotype in heart failure with preserved ejection fraction (HFpEF). However, management of PH-HFpEF is challenging because mechanisms involved in the regulation of PH-HFpEF r…
View article: Endothelial knockdown of the tumor suppressor, <i>WWOX</i> , increases inflammation in ventilator-induced lung injury
Endothelial knockdown of the tumor suppressor, <i>WWOX</i> , increases inflammation in ventilator-induced lung injury Open
Prior tobacco smoke exposure is associated with an increased risk of acute respiratory distress syndrome (ARDS) during critical illness. Our laboratory is investigating one of the gene expression changes that occurs in the lung following s…
View article: NOVEL ECHOCARDIOGRAM ANALYSIS OF CARDIAC DYSFUNCTION IS ASSOCIATED WITH MORTALITY IN PEDIATRIC SEPSIS
NOVEL ECHOCARDIOGRAM ANALYSIS OF CARDIAC DYSFUNCTION IS ASSOCIATED WITH MORTALITY IN PEDIATRIC SEPSIS Open
Objectives: The objective of our study was to semiautomatically generate echocardiogram indices in pediatric sepsis using novel algorithms and determine which indices were associated with mortality. We hypothesized that strain and diastoli…
View article: Gene expression changes in sickle cell reticulocytes and their clinical associations
Gene expression changes in sickle cell reticulocytes and their clinical associations Open
Transcriptional changes in compensatory erythropoiesis in sickle cell anemia (SCA) and their disease modulation are unclear. We detected 1226 differentially expressed genes in hemoglobin SS reticulocytes compared to non-anemic hemoglobin A…
View article: Endothelial Knockdown of the Tumor Suppressor, WWOX, Increases Inflammation in Ventilator-Induced Lung Injury
Endothelial Knockdown of the Tumor Suppressor, WWOX, Increases Inflammation in Ventilator-Induced Lung Injury Open
Background Chronic cigarette smoke exposure downregulates lung expression of WWOX, an ARDS relevant tumor suppressor. Prior work has revealed a barrier protective function of WWOX during infectious models of ARDS. Proteomic analysis of WWO…
View article: Bound Together: RNA Binding Protein Quaking, STAT3, and Pulmonary Vascular Remodeling
Bound Together: RNA Binding Protein Quaking, STAT3, and Pulmonary Vascular Remodeling Open
"Bound Together: RNA Binding Protein Quaking, Stat3, and Pulmonary Vascular Remodeling." American Journal of Respiratory Cell and Molecular Biology, 0(ja), pp.
View article: Human Endogenous Retrovirus, SARS-CoV-2, and HIV Promote PAH via Inflammation and Growth Stimulation
Human Endogenous Retrovirus, SARS-CoV-2, and HIV Promote PAH via Inflammation and Growth Stimulation Open
Pulmonary arterial hypertension (PAH) is a pulmonary vascular disease characterized by the progressive elevation of pulmonary arterial pressures. It is becoming increasingly apparent that inflammation contributes to the pathogenesis and pr…
View article: <i>RASA3</i> is a candidate gene in sickle cell disease‐associated pulmonary hypertension and pulmonary arterial hypertension
<i>RASA3</i> is a candidate gene in sickle cell disease‐associated pulmonary hypertension and pulmonary arterial hypertension Open
Pulmonary hypertension (PH) is associated with significant morbidity and mortality. RASA3 is a GTPase activating protein integral to angiogenesis and endothelial barrier function. In this study, we explore the association of RASA3 genetic …
View article: Recommendations and optimal approaches to robotic-assisted partial nephrectomy: A consensus of Brazilian experts
Recommendations and optimal approaches to robotic-assisted partial nephrectomy: A consensus of Brazilian experts Open
Objective Robotic-assisted partial nephrectomy (RAPN) is established as the gold standard approach to treating small renal masses. However, numerous technical challenges and concepts related to this approach are still under discussion and …
View article: Sphingosine Kinase 1 Deficiency in Smooth Muscle Cells Protects against Hypoxia-Mediated Pulmonary Hypertension via YAP1 Signaling
Sphingosine Kinase 1 Deficiency in Smooth Muscle Cells Protects against Hypoxia-Mediated Pulmonary Hypertension via YAP1 Signaling Open
Sphingosine kinase 1 (SPHK1) and the sphingosine-1-phosphate (S1P) signaling pathway have been shown to play a role in pulmonary arterial hypertension (PAH). S1P is an important stimulus for pulmonary artery smooth muscle cell (PASMC) prol…
View article: Haptoglobin 1 allele predicts higher serum haptoglobin concentration and lower multiorgan failure risk in sickle cell disease
Haptoglobin 1 allele predicts higher serum haptoglobin concentration and lower multiorgan failure risk in sickle cell disease Open
Haptoglobin (HP) is an acute-phase protein and the main scavenger of cell-free hemoglobin. When HP is depleted, as observed in hemolytic conditions such as sickle cell disease (SCD), cell-free hemoglobin can lead to acute organ damage. The…
View article: Cortactin loss protects against hemin-induced acute lung injury in sickle cell disease
Cortactin loss protects against hemin-induced acute lung injury in sickle cell disease Open
In patients with sickle cell disease (SCD), acute chest syndrome (ACS) is a common form of acute lung injury and a major cause of morbidity and mortality. The pathophysiology of ACS is complex, and hemin, the prosthetic moiety of hemoglobi…
View article: Signal Transduction during Metabolic and Inflammatory Reprogramming in Pulmonary Vascular Remodeling
Signal Transduction during Metabolic and Inflammatory Reprogramming in Pulmonary Vascular Remodeling Open
Pulmonary arterial hypertension (PAH) is a progressive disease characterized by (mal)adaptive remodeling of the pulmonary vasculature, which is associated with inflammation, fibrosis, thrombosis, and neovascularization. Vascular remodeling…
View article: Hyperkalemia and Metabolic Acidosis Occur at a Higher eGFR in Sickle Cell Disease
Hyperkalemia and Metabolic Acidosis Occur at a Higher eGFR in Sickle Cell Disease Open
Background People with sickle cell disease (SCD) have an elevated estimated glomerular filtration rate (eGFR) compared with the general population, and this may alter the usual creatinine-based eGFR cutoffs for which physiologic evidence o…
View article: Exercise‐induced changes of vital signs in adults with sickle cell disease
Exercise‐induced changes of vital signs in adults with sickle cell disease Open
The six‐minute walk test (6MWT) has been used in patients with sickle cell disease (SCD), in conjunction with tricuspid regurgitant velocity (TRV) and plasma N‐terminal pro‐brain natriuretic peptide (NT‐pro BNP), to assess risk of having p…
View article: Biomarkers of clinical severity in treated and untreated sickle cell disease: a comparison by genotypes of a single center cohort and African Americans in the NHANES study
Biomarkers of clinical severity in treated and untreated sickle cell disease: a comparison by genotypes of a single center cohort and African Americans in the NHANES study Open
Summary Haemolysis and vaso‐occlusion underlie multi‐organ system complications in sickle cell disease (SCD). We assessed real‐world biomarkers in University of Illinois adult SCD patients, categorised as severe (HbSS/Sβ 0 ‐thalassaemia; n…
View article: The different facets of sickle cell disease-related pulmonary hypertension
The different facets of sickle cell disease-related pulmonary hypertension Open
Purpose of review Sickle cell disease (SCD), one of the most common genetic diseases in the world, is characterized by repeated episodes of hemolysis and vaso-occlusion. Hemolytic anemia is a risk factor for the development of pulmonary hy…
View article: A E2F1/Sphk1/HIF1α Regularly Loop Promotes PASMC Proliferation
A E2F1/Sphk1/HIF1α Regularly Loop Promotes PASMC Proliferation Open
Rationale: Pulmonary hypertension (PH) is characterized by pulmonary vascular remodeling.E2F1 is a transcription factor that participates in many diseases.We have found that hypoxia induces E2F1 expression in human pulmonary arterial smoot…
View article: WWOX Downregulation Induces Suppression of Mitochondrial Activity in Pulmonary Arterial Smooth Muscle Cells
WWOX Downregulation Induces Suppression of Mitochondrial Activity in Pulmonary Arterial Smooth Muscle Cells Open
Introduction: Pulmonary arterial hypertension (PAH) is characterized by excessive proliferation and apoptosis resistance of pulmonary artery smooth muscle cells (PASMCs) which contribute to pulmonary vascular remodeling.These processes are…