Penney M. Gilbert
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View article: Complement-independent pathogenic influences of anti-HMGCR + and anti-SRP + immune-mediated necrotizing myopathy autoantibodies on engineered muscle function
Complement-independent pathogenic influences of anti-HMGCR + and anti-SRP + immune-mediated necrotizing myopathy autoantibodies on engineered muscle function Open
Background Immune-mediated necrotizing myopathy (IMNM) is a subgroup of idiopathic inflammatory myopathies associated with anti-signal recognition particle (SRP) or anti-3-hydroxy-3-methylglutaryl-CoA reductase (HMGCR) autoantibodies. The …
View article: Clinical risk factors of intensive care unit acquired weakness predicted by human muscle microtissue response to humoral factors
Clinical risk factors of intensive care unit acquired weakness predicted by human muscle microtissue response to humoral factors Open
1. Abstract ICUAW is an acquired phenomenon in the critically ill patient that is characterized by severe muscle weakness and atrophy. It results from both myopathic and neuropathic injury and is associated with heightened patient morbidit…
View article: Differentially expressed fusogens specify myocyte states to drive myogenesis
Differentially expressed fusogens specify myocyte states to drive myogenesis Open
During myogenesis, myocyte fusion leads to the formation of multinucleated muscle fibers, but how exactly this process is initiated remains poorly understood. Here, we performed single-cell RNA-sequencing on mouse somites from E9.5-E11.5 e…
View article: Stable isotope tracer captures the anabolic response of human skeletal muscle microtissues undetected by puromycin labeling
Stable isotope tracer captures the anabolic response of human skeletal muscle microtissues undetected by puromycin labeling Open
Skeletal muscle microtissues are valuable in vitro models for studying the stimuli regulating muscle protein synthesis (MPS), the key determinant of changes in muscle mass. Differentiated between opposing posts, microtissues contain aligne…
View article: Elevated GLUT4 Levels in Human Skeletal Muscle Microtissues is Accompanied by Functional Insulin Dependence
Elevated GLUT4 Levels in Human Skeletal Muscle Microtissues is Accompanied by Functional Insulin Dependence Open
Insulin resistance in skeletal muscle is a hallmark of type 2 diabetes mellitus (T2D). While two-dimensional myotube cultures offer a controlled environment for studying T2D-related metabolic dysfunction, insulin-dependent glucose transpor…
View article: Optimizing electrical field stimulation parameters reveals the maximum contractile function of human skeletal muscle microtissues
Optimizing electrical field stimulation parameters reveals the maximum contractile function of human skeletal muscle microtissues Open
Electrical field stimulation (EFS) parameters remain to be standardized for engineered skeletal muscle. Herein, we report a protocol for defining EFS parameters that elicit the maximal contractile force of muscle microtissues cultivated in…
View article: Distinct pathogenic influence of anti-HMGCR+ and anti-SRP+ immune-mediated necrotizing myopathy autoantibodies on engineered muscle function
Distinct pathogenic influence of anti-HMGCR+ and anti-SRP+ immune-mediated necrotizing myopathy autoantibodies on engineered muscle function Open
Immune-mediated necrotizing myopathy (IMNM) is a subgroup of idiopathic inflammatory myopathies associated with anti-signal recognition particle (SRP) or anti-3-hydroxy-3-methylglutaryl-CoA reductase (HMGCR) autoantibodies. However, the de…
View article: Integrating<i>in silico</i>predictions with an engineered tissue assay identifies Perlecan as an age-perturbed re-quiescence cue for muscle stem cells
Integrating<i>in silico</i>predictions with an engineered tissue assay identifies Perlecan as an age-perturbed re-quiescence cue for muscle stem cells Open
Skeletal muscle regeneration is mediated by resident muscle stem cells that produce progeny to repair or recreate muscle. Critical to this function is the ability to transition between states of proliferation and quiescence. This balancing…
View article: A Cryopreservation Strategy for Myoblast Storage in Paper‐Based Scaffolds for Inter‐Laboratory Studies of Skeletal Muscle Health
A Cryopreservation Strategy for Myoblast Storage in Paper‐Based Scaffolds for Inter‐Laboratory Studies of Skeletal Muscle Health Open
3D tissue‐engineered models are poised to facilitate understanding of skeletal muscle pathophysiology and identify novel therapeutic agents to improve muscle health. Adopting these culture models within the broader biology community is a c…
View article: Mini-MEndR: a miniaturized 96-well predictive assay to evaluate muscle stem cell-mediated repair
Mini-MEndR: a miniaturized 96-well predictive assay to evaluate muscle stem cell-mediated repair Open
Background Functional evaluation of molecules that are predicted to promote stem cell mediated endogenous repair often requires in vivo transplant studies that are low throughput and hinder the rate of discovery. To offer greater throughpu…
View article: FAST-STEM: A human pluripotent stem cell engineering toolkit for rapid design-build-test-learn development of human cell-based therapeutic devices
FAST-STEM: A human pluripotent stem cell engineering toolkit for rapid design-build-test-learn development of human cell-based therapeutic devices Open
Very recent clinical advances in stem cell derived tissue replacement and gene therapy, in addition to the rise of artificial intelligence aided scientific discovery, have placed the possibility of sophisticated human cell based therapies …
View article: A cryopreservation strategy for myoblast storage in paper-based scaffolds for inter-laboratory studies of skeletal muscle health
A cryopreservation strategy for myoblast storage in paper-based scaffolds for inter-laboratory studies of skeletal muscle health Open
Three-dimensional tissue-engineered models are poised to facilitate understanding of skeletal muscle pathophysiology and identify novel therapeutic agents to improve muscle health. Adopting these culture models within the broader biology c…
View article: Culture substrate stiffness impacts human myoblast contractility-dependent proliferation and nuclear envelope wrinkling
Culture substrate stiffness impacts human myoblast contractility-dependent proliferation and nuclear envelope wrinkling Open
Understanding how biophysical and biochemical microenvironmental cues together influence the regenerative activities of muscle stem cells and their progeny is crucial in strategizing remedies for pathological dysregulation of these cues in…
View article: Electron microscopic analysis of the influence of iPSC-derived motor neurons on bioengineered human skeletal muscle tissues
Electron microscopic analysis of the influence of iPSC-derived motor neurons on bioengineered human skeletal muscle tissues Open
View article: FAST-STEM: Synthetic Biology Toolkit for Next Generation Regenerative Medicine
FAST-STEM: Synthetic Biology Toolkit for Next Generation Regenerative Medicine Open
View article: Culture substrate stiffness impacts human myoblast contractility-dependent proliferation and nuclear envelope wrinkling
Culture substrate stiffness impacts human myoblast contractility-dependent proliferation and nuclear envelope wrinkling Open
The remarkable self-repair ability of skeletal muscle tissues is driven by muscle stem cells, whose activities are orchestrated by a variety of transient cues present in their microenvironment. Understanding how the cross-interactions of t…
View article: A Flexible Transgene Integration ‘Landing-Pad’ Toolkit in Human Induced Pluripotent Stem Cells Enables Facile Cellular Engineering, Gene Zygosity Control, and Parallel Transgene Integration
A Flexible Transgene Integration ‘Landing-Pad’ Toolkit in Human Induced Pluripotent Stem Cells Enables Facile Cellular Engineering, Gene Zygosity Control, and Parallel Transgene Integration Open
Development of a repeatable method for delivering transgene payloads to human induced pluripotent stem cells (hiPSCs) without risking unintended off-target effects is not fully realized. Yet, such methods are indispensable to fully unlocki…
View article: Electron microscopic analysis of the influence of iPSC-derived motor neurons on bioengineered human skeletal muscle tissues
Electron microscopic analysis of the influence of iPSC-derived motor neurons on bioengineered human skeletal muscle tissues Open
3D bioengineered skeletal muscle macrotissues are increasingly important for studies of cell biology and development of therapeutics. Tissues derived from immortalized cells obtained from patient samples or from stem cells can be co-cultur…
View article: mini-MEndR: A 96-well mixed species culture assay to co-evaluate human and mouse Pax7<sup>+</sup>cell-mediated skeletal muscle repair
mini-MEndR: A 96-well mixed species culture assay to co-evaluate human and mouse Pax7<sup>+</sup>cell-mediated skeletal muscle repair Open
Functional evaluation of molecules that are predicted to promote stem cell mediated endogenous repair often requires in vivo transplant studies that are low throughput and hinder the rate of discovery. Here, we offer a strategy to rapidly …
View article: Dystrophin is a mechanical tension modulator
Dystrophin is a mechanical tension modulator Open
Duchenne muscular dystrophy (DMD) represents the most common inherited muscular disease, where increasing muscle weakness leads to loss of ambulation and premature death. DMD is caused by mutations in the dystrophin gene, and is known to r…
View article: The mini-IDLE 3D biomimetic culture assay enables interrogation of mechanisms governing muscle stem cell quiescence and niche repopulation
The mini-IDLE 3D biomimetic culture assay enables interrogation of mechanisms governing muscle stem cell quiescence and niche repopulation Open
Adult skeletal muscle harbours a population of muscle stem cells (MuSCs) that are required for repair after tissue injury. In youth, MuSCs return to a reversible state of cell-cycle arrest termed ‘quiescence’ after injury resolution. Conve…
View article: Author response: The mini-IDLE 3D biomimetic culture assay enables interrogation of mechanisms governing muscle stem cell quiescence and niche repopulation
Author response: The mini-IDLE 3D biomimetic culture assay enables interrogation of mechanisms governing muscle stem cell quiescence and niche repopulation Open
Article Figures and data Abstract Editor's evaluation eLife digest Introduction Results Discussion Materials and methods Data availability References Decision letter Author response Article and author information Metrics Abstract Adult ske…
View article: Synthetic gene circuits for cell state detection and protein tuning in human pluripotent stem cells
Synthetic gene circuits for cell state detection and protein tuning in human pluripotent stem cells Open
View article: Rescue of aged muscle stem cell quiescence defects by AKT inhibition revealed with a 3D biomimetic culture assay
Rescue of aged muscle stem cell quiescence defects by AKT inhibition revealed with a 3D biomimetic culture assay Open
Adult skeletal muscle harbors a population of muscle stem cells (MuSCs) that are required to repair or reform multinucleated myofibers after tissue injury. In youth, MuSCs return to a reversible state of cell cycle arrest termed ‘quiescenc…
View article: An injury-responsive Rac-to-Rho GTPase switch drives activation of muscle stem cells through rapid cytoskeletal remodeling
An injury-responsive Rac-to-Rho GTPase switch drives activation of muscle stem cells through rapid cytoskeletal remodeling Open
View article: A three-dimensional human adipocyte model of fatty acid-induced obesity
A three-dimensional human adipocyte model of fatty acid-induced obesity Open
Obesity prevalence has reached pandemic proportions, leaving individuals at high risk for the development of diseases such as cancer and type 2 diabetes. In obesity, to accommodate excess lipid storage, adipocytes become hypertrophic, whic…
View article: Discrete-to-analog signal conversion in human pluripotent stem cells
Discrete-to-analog signal conversion in human pluripotent stem cells Open
During development, state transitions are coordinated through changes in the identity of molecular regulators in a cell state- and dose specific manner. The ability to rationally engineer such functions in human pluripotent stem cells (hPS…
View article: Electrophysiological analysis of healthy and dystrophic 3-D bioengineered skeletal muscle tissues
Electrophysiological analysis of healthy and dystrophic 3-D bioengineered skeletal muscle tissues Open
Recently, methods for creating three-dimensional (3-D) human skeletal muscle tissues from myogenic cell lines have been reported. Bioengineered muscle tissues are contractile and respond to electrical and chemical stimulation. In this stud…
View article: TGFβ signaling curbs cell fusion and muscle regeneration
TGFβ signaling curbs cell fusion and muscle regeneration Open
View article: Global and local tension measurements in biomimetic skeletal muscle tissues reveals early mechanical homeostasis
Global and local tension measurements in biomimetic skeletal muscle tissues reveals early mechanical homeostasis Open
Tension and mechanical properties of muscle tissue are tightly related to proper skeletal muscle function, which makes experimental access to the biomechanics of muscle tissue formation a key requirement to advance our understanding of mus…