Falk Schneider
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View article: StayRose: A photostable StayGold derivative redshifted by genetic code expansion
StayRose: A photostable StayGold derivative redshifted by genetic code expansion Open
View article: Prolactin receptor localization and dynamics: Insights from quantitative imaging and mathematical modeling
Prolactin receptor localization and dynamics: Insights from quantitative imaging and mathematical modeling Open
Signal transduction through the prolactin receptor (PRLR) is crucial in pancreatic β-cell pro-liferation, impacting pancreatic homeostasis. PRLR-induced JAK/STAT signaling is dynamic, involving changes in spatial organization of signaling …
View article: Viability assessment and biomass valorization of recombinant Komagataella phaffii
Viability assessment and biomass valorization of recombinant Komagataella phaffii Open
Komagataella phaffii has become a key player in biotechnology used for the production of heterologous proteins with numerous GRAS notices and QPS status released in the past years. While most of these descriptions and scientific studies fo…
View article: Monolagen‐Spezifische Struktur und Dynamik von Oberflächen‐ und Polymer‐Gestützten Lipiddoppelschichten
Monolagen‐Spezifische Struktur und Dynamik von Oberflächen‐ und Polymer‐Gestützten Lipiddoppelschichten Open
Kurzzusammenfassung Polymergestützte oder polymergekoppelte (polymer tethered) Lipid‐Doppelschichten dienen als vielseitige Modellsysteme für die Struktur und Dynamik von Plasmamembranen, doch der Einfluss der Polymerkopplung auf die Eigen…
View article: Leaflet‐Specific Structure and Dynamics of Solid and Polymer Supported Lipid Bilayers
Leaflet‐Specific Structure and Dynamics of Solid and Polymer Supported Lipid Bilayers Open
Polymer‐supported or tethered lipid bilayers serve as versatile platforms for mimicking plasma membrane structure and dynamics, yet the impact of polymer supports on lipid bilayers remains largely unresolved. In this study, we introduce a …
View article: Tutorial: fluorescence lifetime microscopy of membrane mechanosensitive Flipper probes
Tutorial: fluorescence lifetime microscopy of membrane mechanosensitive Flipper probes Open
View article: Quantifying biomolecular organisation in membranes with brightness-transit statistics
Quantifying biomolecular organisation in membranes with brightness-transit statistics Open
View article: More than double the fun with two-photon excitation microscopy
More than double the fun with two-photon excitation microscopy Open
View article: Graphical Abstract: Angew. Chem. Int. Ed. 10/2024
Graphical Abstract: Angew. Chem. Int. Ed. 10/2024 Open
In their Research Article (e202314112), Andreas Dreuw
View article: Far-red chemigenetic biosensors for multi-dimensional and super-resolved kinase activity imaging
Far-red chemigenetic biosensors for multi-dimensional and super-resolved kinase activity imaging Open
Fluorescent biosensors revolutionized biomedical science by enabling the direct measurement of signaling activities in living cells, yet the current technology is limited in resolution and dimensionality. Here, we introduce highly sensitiv…
View article: Single-cell phenomics reveals behavioural and mechanical heterogeneities underpinning collective migration during mouse anterior patterning
Single-cell phenomics reveals behavioural and mechanical heterogeneities underpinning collective migration during mouse anterior patterning Open
SUMMARY Distal Visceral Endoderm (DVE) cells show a stereotypic unidirectional migration essential for correct orientation of the anterior-posterior axis. They migrate within a simple epithelium, the Visceral Endoderm (VE). It is unknown h…
View article: Investigating Diffusion Dynamics and Interactions with Scanning Fluorescence Correlation Spectroscopy (sFCS)
Investigating Diffusion Dynamics and Interactions with Scanning Fluorescence Correlation Spectroscopy (sFCS) Open
Activation of immune cells and formation of immunological synapses (IS) rely critically on the reorganization of the plasma membrane. These highly orchestrated processes are driven by diffusion and oligomerization dynamics, as well as by s…
View article: The kinase occupancy of T cell coreceptors reconsidered
The kinase occupancy of T cell coreceptors reconsidered Open
The sensitivity of the αβ T cell receptor (TCR) is enhanced by the coreceptors CD4 and CD8αβ, which are expressed primarily by cells of the helper and cytotoxic T cell lineages, respectively. The coreceptors bind to major histocompatibilit…
View article: Untreated water from storm sewers as source of microplastic in an urban river system in Taiwan
Untreated water from storm sewers as source of microplastic in an urban river system in Taiwan Open
Microplastic pollution is ubiquitous and affects all environments. In recent years it was found that rivers play a major role in the transport of microplastic to the ocean. Research indicates that human influence is a key factor for microp…
View article: Technical insights into fluorescence lifetime microscopy of mechanosensitive Flipper probes
Technical insights into fluorescence lifetime microscopy of mechanosensitive Flipper probes Open
Measuring forces within living cells remains a technical challenge. We developed hydrophobic mechanosensing fluorescent probes called Flippers, whose fluorescence lifetime depends on lipid packing and can report on membrane tension. Here, …
View article: The kinase occupancy of T-cell coreceptors reconsidered
The kinase occupancy of T-cell coreceptors reconsidered Open
The sensitivity of the αβ T-cell receptor (TCR) is enhanced by the coreceptors CD4 and CD8αβ, which are expressed primarily by cells of the helper and cytotoxic T-cell lineages, respectively. The coreceptors bind to major histocompatibilit…
View article: Diffusion and interaction dynamics of the cytosolic peroxisomal import receptor PEX5
Diffusion and interaction dynamics of the cytosolic peroxisomal import receptor PEX5 Open
View article: ASPP2 maintains the integrity of mechanically stressed pseudostratified epithelia during morphogenesis
ASPP2 maintains the integrity of mechanically stressed pseudostratified epithelia during morphogenesis Open
View article: Microplastic in the Surface Waters of Rural and Urban River Sections: Correlation with Land Use and the Role of Storm Sewers as Potential Pathways
Microplastic in the Surface Waters of Rural and Urban River Sections: Correlation with Land Use and the Role of Storm Sewers as Potential Pathways Open
View article: Aggregation and mobility of membrane proteins interplay with local lipid order in the plasma membrane of T cells
Aggregation and mobility of membrane proteins interplay with local lipid order in the plasma membrane of T cells Open
To disentangle the elusive lipid–protein interactions in T‐cell activation, we investigate how externally imposed variations in mobility of key membrane proteins (T‐cell receptor [TCR], kinase Lck, and phosphatase CD45) affect the local li…
View article: Two-dimensional TIRF-SIM–traction force microscopy (2D TIRF-SIM-TFM)
Two-dimensional TIRF-SIM–traction force microscopy (2D TIRF-SIM-TFM) Open
View article: Quantitative Bio-Imaging Tools to Dissect the Interplay of Membrane and Cytoskeletal Actin Dynamics in Immune Cells
Quantitative Bio-Imaging Tools to Dissect the Interplay of Membrane and Cytoskeletal Actin Dynamics in Immune Cells Open
Cellular function is reliant on the dynamic interplay between the plasma membrane and the actin cytoskeleton. This critical relationship is of particular importance in immune cells, where both the cytoskeleton and the plasma membrane work …
View article: Aggregation and immobilisation of membrane proteins interplay with local lipid order in the plasma membrane of T cells
Aggregation and immobilisation of membrane proteins interplay with local lipid order in the plasma membrane of T cells Open
The quest for understanding of numerous vital membrane-associated cellular processes, such as signalling, has largely focussed on the spatiotemporal orchestration and reorganisation of the identified key proteins, including their binding a…
View article: Influence of nanobody binding on fluorescence emission, mobility, and organization of GFP-tagged proteins
Influence of nanobody binding on fluorescence emission, mobility, and organization of GFP-tagged proteins Open
View article: Studies on fixed-time ovulation induction in the pig
Studies on fixed-time ovulation induction in the pig Open
A technology that allows for manipulating of oestrus and ovulation, and would then also allow for fixed-time insemination, can be of great benefit for swine farms that operate using sow batch management due, at least in part, to savings in…
View article: Influence of nanobody binding on fluorescence emission, mobility and organization of GFP-tagged proteins
Influence of nanobody binding on fluorescence emission, mobility and organization of GFP-tagged proteins Open
Summary Advanced fluorescence microscopy studies require specific and monovalent molecular labelling with bright and photostable fluorophores. This necessity led to the widespread use of fluorescently labelled nanobodies against commonly e…
View article: The cortical actin network regulates avidity-dependent binding of hyaluronan by the lymphatic vessel endothelial receptor LYVE-1
The cortical actin network regulates avidity-dependent binding of hyaluronan by the lymphatic vessel endothelial receptor LYVE-1 Open
Lymphatic vessel endothelial hyaluronan receptor 1 (LYVE-1) mediates the docking and entry of dendritic cells to lymphatic vessels through selective adhesion to its ligand hyaluronan in the leukocyte surface glycocalyx. To bind hyaluronan …
View article: High photon count rates improve the quality of super-resolution fluorescence fluctuation spectroscopy
High photon count rates improve the quality of super-resolution fluorescence fluctuation spectroscopy Open
Probing the diffusion of molecules has become a routine measurement across the life sciences, chemistry and physics. It provides valuable insights into reaction dynamics, oligomerisation, molecular (re-)organisation or cellular heterogenei…
View article: Plasma membrane dynamics in immune signalling
Plasma membrane dynamics in immune signalling Open
The cellular plasma membrane encloses all mammalian cells and is a heterogeneous structure organised on small length- and short timescales. Separation from the exterior milieu allows non-equilibrium processes and renders the membrane the f…
View article: Sterile activation of invariant natural killer T cells by ER-stressed antigen-presenting cells
Sterile activation of invariant natural killer T cells by ER-stressed antigen-presenting cells Open
Significance While there is a clear understanding of how invariant NKT (iNKT) cells are activated in foreign infection, it remains unclear how they are activated during sterile inflammation, including cancer, where they have a well-defined…