Explanipedia

Lactylated Proteomic Analysis Reveals Functional Implications of Lysine Lactylation In Asthenozoospermia Open

Minghao Yan, Haixia Tu, Shanshan Tang, Zheng Gai, Qianqian Shi , et al. · 2025

The mechanism underlying asthenozoospermia in male infertility has been a prominent topic in reproductive medicine research. Human sperm function is modified by various protein post-translational modifications (PTMs). Among these, lactylat…

Separately prestored proteasome components to prevent polyspermy Open

Wei Li, Liying Wang, Xing Wang, Yueshuai Guo, Yueshuai Guo , et al. · 2025

To ensure genome stability across generations, an egg must be fertilized by only a single sperm. However, how sperm actively contribute to preventing polyspermy has remained unknown. Here, we demonstrate that testis-specific 20S proteasome…

Stage‐Resolved Phosphoproteomic Landscape of Mouse Spermiogenesis Reveals Key Kinase Signaling in Sperm Morphogenesis Open

Tianyu Zhu, Yan Zhu, Xinyi Jiang, Xiangzheng Zhang, Bing Wang , et al. · 2025

Spermiogenesis is the committed step of sperm production, during which spermatid cells undergo dramatic morphological transformations and transcriptional silencing. Post‐translational modifications (PTMs), including phosphorylation, provid…

A functionally tunable magnetic nanochains platform for N-glycoproteomic analysis of extracellular vesicles from ultratrace biofluids Open

Mingyang Li, Xiaorong Song, Haixia Tu, Jie Sun, Yifei Deng , et al. · 2025

Extracellular vesicles (EVs) carry complex glycoproteins critical mediators of disease progression and hold great potential for liquid biopsy applications. However, their low abundance presents significant analytical challenges. This study…

A Novel Cultivation System for Germ Cell Proliferation and Sustaining Whole Testicular Niche Open

Yu Xia, Xiaoxuan Zhang, Bohang Zhang, Minhui Lu, Qing Cheng , et al. · 2025

Childhood cancer treatments often impair male fertility due to gonadotoxic effects. While in vitro culture of prepubertal testis tissue offers a potential solution to preserve fertility, producing sufficient sperm remains a major barrier. …

Genome-wide survey of the HMA gene family in wheat (Triticum aestivum L.) and its potential role in cadmium stress Open

Leilei Shao, Xuejiang Guo, Yang Wang, Ting Lei, Yongshan Wan , et al. · 2025

Cadmium has been accumulating in the agricultural and ecological environment in recent years due to the release of industrial pollutants. Due to its high solubility, slow degradability and high toxicity, it is highly susceptible to occurri…

Genome-wide survey of the HMA gene family in wheat (Triticum aestivum) and its potential role in cadmium stress Open

Leilei Shao, Xuejiang Guo, Yang Wang, Ting Lei, Yongshan Wan , et al. · 2025

Cadmium has been accumulating in the agricultural and ecological environment in recent years due to the release of industrial pollutants. Due to its high solubility, slow degradability and high toxicity, it is highly susceptible to occurri…

Trans-ethnic GWAS meta-analysis of idiopathic spermatogenic failure highlights the immune-mediated nature of Sertoli cell-only syndrome Open

Sara González-Muñoz, Long Yi, Andrea Guzmán‐Jiménez, Miriam Cerván‐Martín, Inmaculada Higueras-Serrano , et al. · 2025

circASbase: A Comprehensive Database of Alternative Splicing Events in circRNAs Open

Lingxiao Zou, Jian Zhao, H. Li, Xu Chen, Y. Wang , et al. · 2025

Despite extensive studies highlight the critical roles of alternative splicing in generating mature circRNA isoforms and enhancing their function diversity, a significant gap remains in the availability of dedicated databases for circRNA a…

Multi‐Omics Reveal the Metabolic Changes in Cumulus Cells During Aging Open

Liangyue Shi, Hengjie Wang, Shuai Zhu, Minjian Chen, Xuejiang Guo , et al. · 2025

Maternal age has been reported to impair oocyte quality. However, the molecular mechanisms underlying the age‐related decrease in oocyte competence remain poorly understood. Cumulus cells establish direct contact with the oocyte through ga…

PAK4 promotes the cytoskeletal organization and meiotic maturation via phosphorylating DDX17 in oocyte Open

Hengjie Wang, Ming Gao, Qing Cheng, Shuai Zhu, Yu Chen , et al. · 2025

Single-Cell Multi-Omics Analysis of In Vitro Post-Ovulatory–Aged Oocytes Revealed Aging-Dependent Protein Degradation Open

Yueshuai Guo, Mengmeng Gao, Xiaofei Liu, Haotian Zhang, Yue Wang , et al. · 2024

Integrative Omics Reveals the Metabolic Patterns During Oocyte Growth Open

Xiang Zhang, Juan Ge, Yue Wang, Minjian Chen, Xuejiang Guo , et al. · 2024

The global phosphorylation landscape of mouse oocytes during meiotic maturation Open

Hongzheng Sun, Longsen Han, Yueshuai Guo, Huiqing An, Bing Wang , et al. · 2024

Phosphorylation is a key post-translational modification regulating protein function and biological outcomes. However, the phosphorylation dynamics orchestrating mammalian oocyte development remains poorly understood. In the present study,…

BERT and LLMs-Based avGFP Brightness Prediction and Mutation Design Open

Xuejiang Guo, Wanxiang Che · 2024

This study aims to utilize Transformer models and large language models (such as GPT and Claude) to predict the brightness of Aequorea victoria green fluorescent protein (avGFP) and design mutants with higher brightness. Considering the ti…

MIB2 Functions in Oocyte Meiosis by Modulating Chromatin Configuration Open

Yifei Jin, Sun Guangyi, Jiashuo Li, Qing Cheng, Hongzheng Sun , et al. · 2024

Quantitative Phosphoproteomic Profiling of Mouse Sperm Maturation in Epididymis Revealed Kinases Important for Sperm Motility Open

Xiangzheng Zhang, Haixia Tu, Xin Zhou, Bing Wang, Yueshuai Guo , et al. · 2024

Transcriptionally and translationally silent sperm undergo functional maturation during epididymis traverse, which provides sperm ability to move and is crucial for successful fertilization. However, the molecular mechanisms governing sper…

Checkpoint Kinase 1 Stimulates Endogenous Cardiomyocyte Renewal and Cardiac Repair by Binding to Pyruvate Kinase Isoform M2 C‐Domain and Activating Cardiac Metabolic Reprogramming in a Porcine Model of Myocardial Ischemia/Reperfusion Injury Open

Tianwen Wei, Tiankai Shan, Hao Wang, Jiawen Chen, Tongtong Yang , et al. · 2024

Background The regenerative capacity of the adult mammalian hearts is limited. Numerous studies have explored mechanisms of adult cardiomyocyte cell‐cycle withdrawal. This translational study evaluated the effects and underlying mechanism …

TransGCN: a semi-supervised graph convolution network–based framework to infer protein translocations in spatio-temporal proteomics Open

Bing Wang, Xiangzheng Zhang, Xudong Han, Bingjie Hao, Yan Li , et al. · 2024

Protein subcellular localization (PSL) is very important in order to understand its functions, and its movement between subcellular niches within cells plays fundamental roles in biological process regulation. Mass spectrometry–based spati…

STYXL1 regulates CCT complex assembly and flagellar tubulin folding in sperm formation Open

Yu Chen, Mengjiao Luo, Haixia Tu, Yaling Qi, Yueshuai Guo , et al. · 2024

Data for EMSL Project 49531 from January 2024 Open