Feifan Leng
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View article: Integrating transcriptome, metabolome and microbiome to explore the interaction mechanism between secondary metabolites and root-associated bacteria of Codonopsis pilosula
Integrating transcriptome, metabolome and microbiome to explore the interaction mechanism between secondary metabolites and root-associated bacteria of Codonopsis pilosula Open
View article: The Mechanisms of Interaction between Alfalfa Root Exudates and Rhizosphere Bacterial Communities for Adaptation to Salt Stress
The Mechanisms of Interaction between Alfalfa Root Exudates and Rhizosphere Bacterial Communities for Adaptation to Salt Stress Open
View article: Development and mechanism exploration of a quantitative model for Escherichia coli transformation efficiency based on ultrasonic power
Development and mechanism exploration of a quantitative model for Escherichia coli transformation efficiency based on ultrasonic power Open
View article: Analysis and Identification of Differences in Volatile Components of Various Alfalfa Seeds Based on GC-IMS
Analysis and Identification of Differences in Volatile Components of Various Alfalfa Seeds Based on GC-IMS Open
Volatile components are one key factor in sample identification, differential analysis, quality control and origin traceability. In order to identify and analyze the differences in volatile substances in different alfalfa seeds, this study…
View article: Comparative production performance and rumen bacterial diversity of fattening beef cattle supplemented with different levels concentrated feed
Comparative production performance and rumen bacterial diversity of fattening beef cattle supplemented with different levels concentrated feed Open
The production performance and rumen bacterial diversity were compared for different silage-based diets supplemented with common concentrate or bio-concentrate to develop an alternative of common concentrate for fatten cattle feeding. The …
View article: Genomic Characteristics and Comparative Genomics Analysis of the Endophytic Fungus Paraphoma chrysanthemicola DS-84 Isolated from Codonopsis pilosula Root
Genomic Characteristics and Comparative Genomics Analysis of the Endophytic Fungus Paraphoma chrysanthemicola DS-84 Isolated from Codonopsis pilosula Root Open
Paraphoma chrysanthemicola is a newly identified endophytic fungus. The focus of most studies on P. chrysanthemicola has been on its isolation, identification and effects on plants. However, the limited genomic information is a barrier to …
View article: Changes of Key Soil Factors, Biochemistry and Bacterial Species Composition during Seasons in the Rhizosphere and Roots of Codonopsis pilosula (tangshen)
Changes of Key Soil Factors, Biochemistry and Bacterial Species Composition during Seasons in the Rhizosphere and Roots of Codonopsis pilosula (tangshen) Open
Codonopsis pilosula is a medicinal and edible herb with a rich nutritional value. In Gansu Province, China, its production quality and yield differ during the four seasons. Here, we investigated the differences in the microfloral compositi…
View article: Protection of primary cilia is an effective countermeasure against the impairment of osteoblast function induced by simulated microgravity
Protection of primary cilia is an effective countermeasure against the impairment of osteoblast function induced by simulated microgravity Open
The molecular mechanism for the microgravity‐induced decrease in bone formation remains unclear and there is a lack of effective specific preventative therapies. We recently reported that primary cilia of osteoblasts became shorter and eve…
View article: Identification of Key Genes during Ca <sup>2+</sup> -Induced Genetic Transformation in <i>Escherichia coli</i> by Combining Multi-Omics and Gene Knockout Techniques
Identification of Key Genes during Ca <sup>2+</sup> -Induced Genetic Transformation in <i>Escherichia coli</i> by Combining Multi-Omics and Gene Knockout Techniques Open
Using transcriptome and proteome techniques and association analysis, we identified several key genes involved in the formation of Ca 2+ -mediated E. coli DH5α competent cells. We used Red homologous recombination technology to construct t…
View article: Insights into the Structures, Inhibitors, and Improvement Strategies of Glucose Oxidase
Insights into the Structures, Inhibitors, and Improvement Strategies of Glucose Oxidase Open
Glucose oxidase, which uses molecular oxygen as an electron acceptor to specifically catalyze the conversion of β-d-glucose to gluconic acid and hydrogen peroxide (H2O2), has been considered an important enzyme in increasing environmental …
View article: Comparative analysis of three kinds of extraction kinetic models of crude polysaccharides from Codonopsis pilosula and evaluate the characteristics of crude polysaccharides
Comparative analysis of three kinds of extraction kinetic models of crude polysaccharides from Codonopsis pilosula and evaluate the characteristics of crude polysaccharides Open
View article: Microbial communities changes across aging process of tobacco leaves
Microbial communities changes across aging process of tobacco leaves Open
Background: Microbial communities that inhabit aging tobacco leaves play a key role in improving quality by interactions with themselves and tobacco leaves to release tannins, increase sugar levels, promote aromatic taste and degrade harmf…
View article: Physiological and Genetic Response Characteristics of Stenotrophomonas Rhizophila JC1 Under Heavy Metal Stress
Physiological and Genetic Response Characteristics of Stenotrophomonas Rhizophila JC1 Under Heavy Metal Stress Open
In this study, the Cu2+ (120 mg/L) and Cr6+(80 mg/L) removal rate of S. rhizophila JC1 reached at 79.9% and 89.3%, respectively. Scanning electron microscopy showed that Pb2+ and Zn2+ had no obvi…
View article: NUTRITIONAL VALUE ANALYSIS OF DIFFERENT SWEET SORGHUM VARIETIES IN DIFFERENT CUTTING PERIODS
NUTRITIONAL VALUE ANALYSIS OF DIFFERENT SWEET SORGHUM VARIETIES IN DIFFERENT CUTTING PERIODS Open
In this study, 14 kinds of different forage sweet sorghum varieties were used as materials to study the effects of different cutting times on the biological yield and nutrient components of Sweet Sorghum, including the determination of pla…
View article: COMPARISON ANALYSIS OF PRODUCTION PERFORMANCE AND RUMEN BACTERIAL DIVERSITY OF FATTENING BEEF CATTLE WITH DIFFERENT DIET FORMULAS
COMPARISON ANALYSIS OF PRODUCTION PERFORMANCE AND RUMEN BACTERIAL DIVERSITY OF FATTENING BEEF CATTLE WITH DIFFERENT DIET FORMULAS Open
The effect of beef cattle (Angus vs. Simmental) feeds with various diet formulas (Corn silage and sorghum silage) on the production performance, blood biochemical indexes, beef quality and Rumen bacterial diversity of were investigated. Co…
View article: Optimization and mechanism exploration for Escherichia coli transformed with plasmid pUC19 by the combination with ultrasound treatment and chemical method
Optimization and mechanism exploration for Escherichia coli transformed with plasmid pUC19 by the combination with ultrasound treatment and chemical method Open
View article: Effects of Sr<sup>2 +</sup> on the preparation of <i>Escherchia coli</i> DH5α competent cells and plasmid transformation
Effects of Sr<sup>2 +</sup> on the preparation of <i>Escherchia coli</i> DH5α competent cells and plasmid transformation Open
Bacterial gene transformation used with Escherichia coli as a desired microorganism is one of the important techniques in genetic engineering. In this study, the preparation of E. coli DH5α competent cells treated with SrCl 2 and transform…
View article: Peer Review #2 of "Expression of Aspergillus niger glucose oxidase in Pichia pastoris and its antimicrobial activity against Agrobacterium and Escherichia coli (v0.1)"
Peer Review #2 of "Expression of Aspergillus niger glucose oxidase in Pichia pastoris and its antimicrobial activity against Agrobacterium and Escherichia coli (v0.1)" Open
The gene encoding glucose oxidase from Aspergillus niger ZM-8 was cloned and transferred to Pichia pastoris GS115, a transgenic strain P. pastoris GS115-His-GOD constructed.The growth curve of P. pastoris GS115-His-GOD was consistent with …
View article: Expression of <i>Aspergillus niger</i> glucose oxidase in <i>Pichia pastoris</i> and its antimicrobial activity against <i>Agrobacterium</i> and <i>Escherichia coli</i>
Expression of <i>Aspergillus niger</i> glucose oxidase in <i>Pichia pastoris</i> and its antimicrobial activity against <i>Agrobacterium</i> and <i>Escherichia coli</i> Open
The gene encoding glucose oxidase from Aspergillus niger ZM-8 was cloned and transferred to Pichia pastoris GS115, a transgenic strain P. pastoris GS115-His-GOD constructed. The growth curve of P. pastoris GS115-His-GOD was consistent with…
View article: Peer Review #1 of "Expression of Aspergillus niger glucose oxidase in Pichia pastoris and its antimicrobial activity against Agrobacterium and Escherichia coli (v0.1)"
Peer Review #1 of "Expression of Aspergillus niger glucose oxidase in Pichia pastoris and its antimicrobial activity against Agrobacterium and Escherichia coli (v0.1)" Open
The gene encoding glucose oxidase from Aspergillus niger ZM-8 was cloned and transferred to Pichia pastoris GS115, a transgenic strain P. pastoris GS115-His-GOD constructed.The growth curve of P. pastoris GS115-His-GOD was consistent with …
View article: Arsenic bioleaching in medical realgar ore and arsenicbearing refractory gold ore by combination of <i>Acidithiobacillus ferrooxidans</i> and <i>Acidithiobacillus thiooxidans</i>
Arsenic bioleaching in medical realgar ore and arsenicbearing refractory gold ore by combination of <i>Acidithiobacillus ferrooxidans</i> and <i>Acidithiobacillus thiooxidans</i> Open
Purpose: To develop a novel biotechnological method for removing toxic arsenic from two kinds of representative arsenic-containing ores using different mixed mesophilic acidophiles. Methods: Bioleaching of the two types of arsenic-containi…