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View article: Simultaneous Protein and RNA Analysis in Single Extracellular Vesicles, Including Viruses
Simultaneous Protein and RNA Analysis in Single Extracellular Vesicles, Including Viruses Open
The individual detection of human immunodeficiency virus (HIV) virions and resolution from extracellular vesicles (EVs) during analysis is a difficult challenge. Infectious enveloped virions and nonviral EVs are released simultaneously by …
View article: Synthetic protein circuits for programmable control of mammalian cell death
Synthetic protein circuits for programmable control of mammalian cell death Open
Natural cell death pathways such as apoptosis and pyroptosis play dual roles: they eliminate harmful cells and modulate the immune system by dampening or stimulating inflammation. Synthetic protein circuits capable of triggering specific d…
View article: Simultaneous protein and RNA analysis in single extracellular vesicles, including viruses: SPIRFISH
Simultaneous protein and RNA analysis in single extracellular vesicles, including viruses: SPIRFISH Open
Interest in using nanoparticles for delivery of therapeutic RNA has been steadily growing, provoking a need to precisely understand their structure and contents. Single-particle and single-molecule analysis techniques provide snapshots of …
View article: Germline-encoded amino acid–binding motifs drive immunodominant public antibody responses
Germline-encoded amino acid–binding motifs drive immunodominant public antibody responses Open
Despite the vast diversity of the antibody repertoire, infected individuals often mount antibody responses to precisely the same epitopes within antigens. The immunological mechanisms underpinning this phenomenon remain unknown. By mapping…
View article: Lineage tracing reveals fate bias and transcriptional memory in human B cells
Lineage tracing reveals fate bias and transcriptional memory in human B cells Open
We combined single-cell transcriptomics and lineage tracing to understand fate choice in human B cells. Using the antibody sequences of B cells, we tracked clones during in vitro differentiation. Clonal analysis revealed a subset of IgM+ B…
View article: Fate Bias and Transcriptional Memory of human B cells
Fate Bias and Transcriptional Memory of human B cells Open
Lineage tracking offers a direct approach to study cell fate determination. In this work we combined single cell transcriptomics and lineage tracing to better understand fate-choice in human B cells. Using the antibody sequence to trace ce…
View article: Fly Cell Atlas: A single-nucleus transcriptomic atlas of the adult fruit fly
Fly Cell Atlas: A single-nucleus transcriptomic atlas of the adult fruit fly Open
For more than 100 years, the fruit fly Drosophila melanogaster has been one of the most studied model organisms. Here, we present a single-cell atlas of the adult fly, Tabula Drosophilae , that includes 580,000 nuclei from 15 individually …
View article: Interactive VISION reports for "KP-Tracer Tumors from study "Lineage Recording Reveals the Phylodynamics, Plasticity and Paths of Tumor Evolution"
Interactive VISION reports for "KP-Tracer Tumors from study "Lineage Recording Reveals the Phylodynamics, Plasticity and Paths of Tumor Evolution" Open
This repository contains VISION reports for the KP-Tracer tumors described in the manuscript "Lineage Recording Reveals the Phylodynamics, Plasticity, and Paths of Tumor Evolution" (Yang*, Jones*, et al bioRxiv 2021). In this study, single…
View article: Interactive VISION reports for "KP-Tracer Tumors from study "Lineage Recording Reveals the Phylodynamics, Plasticity and Paths of Tumor Evolution"
Interactive VISION reports for "KP-Tracer Tumors from study "Lineage Recording Reveals the Phylodynamics, Plasticity and Paths of Tumor Evolution" Open
This repository contains VISION reports for the KP-Tracer tumors described in the manuscript "Lineage Recording Reveals the Phylodynamics, Plasticity, and Paths of Tumor Evolution" (Yang*, Jones*, et al bioRxiv 2021). In this study, single…
View article: Processed data for KP-Tracer Tumors from study "Lineage Recording Reveals the Phylodynamics, Plasticity and Paths of Tumor Evolution"
Processed data for KP-Tracer Tumors from study "Lineage Recording Reveals the Phylodynamics, Plasticity and Paths of Tumor Evolution" Open
This repository contains processed data associated with the manuscript "Lineage Recording Reveals the Phylodynamics, Plasticity, and Paths of Tumor Evolution" (Yang*, Jones*, et al bioRxiv 2021). In this study, single-cell lineage tracing …
View article: Processed data for KP-Tracer Tumors from study "Lineage Recording Reveals the Phylodynamics, Plasticity and Paths of Tumor Evolution"
Processed data for KP-Tracer Tumors from study "Lineage Recording Reveals the Phylodynamics, Plasticity and Paths of Tumor Evolution" Open
This repository contains processed data associated with the manuscript "Lineage Recording Reveals the Phylodynamics, Plasticity, and Paths of Tumor Evolution" (Yang*, Jones*, et al bioRxiv 2021). In this study, single-cell lineage tracing …
View article: Lineage Recording Reveals the Phylodynamics, Plasticity and Paths of Tumor Evolution
Lineage Recording Reveals the Phylodynamics, Plasticity and Paths of Tumor Evolution Open
SUMMARY Tumor evolution is driven by the progressive acquisition of genetic and epigenetic alterations that enable uncontrolled growth, expansion to neighboring and distal tissues, and therapeutic resistance. The study of phylogenetic rela…
View article: Single-cell transcriptomes of developing and adult olfactory receptor neurons in Drosophila
Single-cell transcriptomes of developing and adult olfactory receptor neurons in Drosophila Open
Recognition of environmental cues is essential for the survival of all organisms. Transcriptional changes occur to enable the generation and function of the neural circuits underlying sensory perception. To gain insight into these changes,…
View article: Temporal evolution of single-cell transcriptomes of Drosophila olfactory projection neurons
Temporal evolution of single-cell transcriptomes of Drosophila olfactory projection neurons Open
Neurons undergo substantial morphological and functional changes during development to form precise synaptic connections and acquire specific physiological properties. What are the underlying transcriptomic bases? Here, we obtained the sin…
View article: Author response: Temporal evolution of single-cell transcriptomes of Drosophila olfactory projection neurons
Author response: Temporal evolution of single-cell transcriptomes of Drosophila olfactory projection neurons Open
Article Figures and data Abstract Introduction Results Discussion Materials and methods Data availability References Decision letter Author response Article and author information Metrics Abstract Neurons undergo substantial morphological …
View article: Decision letter: Development of antibody-dependent cell cytotoxicity function in HIV-1 antibodies
Decision letter: Development of antibody-dependent cell cytotoxicity function in HIV-1 antibodies Open
Article Figures and data Abstract eLife digest Introduction Results Discussion Materials and methods Data availability References Decision letter Author response Article and author information Metrics Abstract A prerequisite for the design…
View article: Single-cell transcriptomes of developing and adult olfactory receptor neurons in<i>Drosophila</i>
Single-cell transcriptomes of developing and adult olfactory receptor neurons in<i>Drosophila</i> Open
Recognition of environmental cues is essential for the survival of all organisms. Precise transcriptional changes occur to enable the generation and function of the neural circuits underlying sensory perception. To gain insight into these …
View article: Cloning antibodies from single cells in pooled sequence libraries by selective PCR
Cloning antibodies from single cells in pooled sequence libraries by selective PCR Open
Antibodies function by binding to antigens. Antibodies must be cloned and expressed to determine their binding characteristics, but current methods for high-throughput antibody sequencing yield antibody DNA pooled from many cells and do no…
View article: Transcriptional program of memory B cell activation, broadly binding anti-influenza antibodies, and bystander activation after vaccination revealed by single-cell transcriptomics
Transcriptional program of memory B cell activation, broadly binding anti-influenza antibodies, and bystander activation after vaccination revealed by single-cell transcriptomics Open
Antibody memory protects humans from many diseases. Protective antibody memory responses require activation of transcriptional programs, cell proliferation, and production of antigen-specific antibodies, but how these aspects of the respon…
View article: Coordinating Receptor Expression and Wiring Specificity in Olfactory Receptor Neurons
Coordinating Receptor Expression and Wiring Specificity in Olfactory Receptor Neurons Open
The ultimate function of a neuron is determined by both its physiology and connectivity, but the transcriptional regulatory mechanisms that coordinate these two features are not well understood 1–4 . The Drosophila Olfactory receptor neuro…
View article: Signatures of selection in the human antibody repertoire: Selective sweeps, competing subclones, and neutral drift
Signatures of selection in the human antibody repertoire: Selective sweeps, competing subclones, and neutral drift Open
Antibodies are created and refined by somatic evolution in B cell populations, which endows the human immune system with the ability to recognize and eliminate diverse pathogens. However, the evolutionary processes that sculpt antibody rep…
View article: Activation of polarized cell growth by inhibition of cell polarity
Activation of polarized cell growth by inhibition of cell polarity Open
A key feature of cells is the capacity to activate new functional polarized domains contemporaneously to pre-existing ones. How cells accomplish this is not clear. Here, we show that in fission yeast inhibition of cell polarity at pre-exis…