Explanipedia

Open-source sub-nanometer stabilization system for super-resolution fluorescence microscopy Open

Guillermo P. Acuna, Philip Tinnefeld, Lucía F. Lopez, Andrés Zelcer, Fernando D. Stefani · 2025

Recent advances in fluorescence nanoscopy have pushed resolution to the 1–10 nm range, enabling the direct visualization of individual molecules even in crowded biological environments. Achieving this level of precision requires rigorous s…

Direct single-molecule detection and super-resolution imaging with a low-cost portable smartphone-based microscope Open

Morgane Loretan, Mariano Barella, Nathan Fuchs, Samet Kocabey, Karol Kołątaj , et al. · 2025

Detecting single molecules, which represents the ultimate level of sensitivity, is typically achieved with research-grade equipment. Here we present a low-cost, portable smartphone-based fluorescence microscope capable of detecting single-…

Dynamic relocation of PKA regulatory subunits during sperm capacitation: Linking PKA to the CatSper signaling complex Open

Analia G. Novero, Arturo Matamoros‐Volante, Lucila R. Gomez-Olivieri, Cintia Stival, Guillermina M. Luque , et al. · 2025

To fertilize an oocyte, mammalian spermatozoa must undergo a maturation step known as capacitation that takes place after ejaculation. Protein kinase A (PKA) plays a fundamental role in capacitation in all mammalian species. Before capacit…

Open-source Sub-Nanometer Stabilization System for Super-resolution Fluorescence Microscopy Open

Lucía F. Lopez, Florencia Edorna, Florencia Daniela Choque, Giovanni Ferrari, Luciano A. Masullo , et al. · 2025

Recent advances in fluorescence nanoscopy have pushed resolution to the 1-10 nm range, enabling the direct visualization of individual molecules even in crowded biological environments. Achieving this level of precision requires rigorous s…

Coupling single-molecules to DNA-based optical antennas with position and orientation control Open

Aleksandra K. Adamczyk, Fangjia Zhu, Daniel Schaeafer, Yuya Kanehira, Sergio Kogikoski , et al. · 2024

Optical antennas have been extensively employed to manipulate the photophysical properties of single photon emitters. Coupling between an emitter and a given resonant mode of an optical antenna depends mainly on three parameters: spectral …

Direct single-molecule detection and super-resolution imaging with a low-cost portable smartphone-based microscope Open

Morgane Loretan, Mariano Barella, Nathan Fuchs, Samet Kocabey, Karol Kołątaj , et al. · 2024

We present a novel, low-cost, portable smartphone-based fluorescence microscope capable of directly detecting single molecules without signal amplification. The setup leverages the image sensors and data handling capacity of mass-produced …

Towards full control of molecular exciton energy transfer via FRET in DNA origami assemblies Open

Aleksandra K. Adamczyk, Teun A.P.M. Huijben, Karol Kołątaj, Fangjia Zhu, Rodolphe Marie , et al. · 2024

Controlling the flow of excitons between organic molecules holds immense promise for various applications, including energy conversion, spectroscopy, photocatalysis, sensing, and microscopy. DNA nanotechnology has shown promise in achievin…

Impact of bimetallic interface design on heat generation in plasmonic Au/Pd nanostructures studied by single-particle thermometry Open

Julián Gargiulo, Matías Herran, Ianina L. Violi, Ana Sousa‐Castillo, Luciana P. Martinez , et al. · 2023

Localized surface plasmons are lossy and generate heat. However, accurate measurement of the temperature of metallic nanoparticles under illumination remains an open challenge, creating difficulties in the interpretation of results across …

Super-Resolved FRET Imaging by Confocal Fluorescence-Lifetime Single-Molecule Localization Microscopy Open

Cecilia Zaza, Germán Chiarelli, Ludovit P. Zweifle, Mauricio Pilo‐Pais, Evangelos Sisamakis , et al. · 2023

FRET-based methods are a special tool for detecting interactions between (bio)molecules and their immediate environment. The spatial distribution of molecular interactions and functional states can be seen using FLIM (Fluorescence Lifetime…

Super-Resolved FRET Imaging by Confocal Fluorescence-Lifetime Single-Molecule Localization Microscopy Open

Cecilia Zaza, Germán Chiarelli, Ludovit P. Zweifle, Mauricio Pilo‐Pais, Evangelos Sisamakis , et al. · 2023

FRET-based methods are a special tool for detecting interactions between (bio)molecules and their immediate environment. The spatial distribution of molecular interactions and functional states can be seen using FLIM (Fluorescence Lifetime…

Super‐Resolved FRET Imaging by Confocal Fluorescence‐Lifetime Single‐Molecule Localization Microscopy Open

Cecilia Zaza, Germán Chiarelli, Ludovit Zweifel, Mauricio Pilo‐Pais, Evangelos Sisamakis , et al. · 2023

Fluorescence Resonance Energy Transfer (FRET)‐based approaches are unique tools for sensing the immediate surroundings and interactions of (bio)molecules. FRET imaging and Fluorescence Lifetime Imaging Microscopy (FLIM) enable the visualiz…

Dual spatio-temporal regulation of axon growth and microtubule dynamics by RhoA signaling pathways Open

José Wojnacki, Gonzalo Quassollo, Martín D. Bordenave, Nicolás Unsain, Gaby F. Martínez , et al. · 2023

RhoA plays a crucial role in neuronal polarization, where its action restraining axon outgrowth has been thoroughly studied. We now report that RhoA has not only inhibitory but also a stimulatory effect on axon development depending on whe…

Super-Resolved FRET and Co-Tracking in pMINFLUX Open

Fiona Cole, Jonas Zähringer, Johann Bohlen, Tim Schröder, Florian Steiner , et al. · 2023

Summary Single-molecule FRET (smFRET) is widely used to investigate dynamic (bio) molecular interactions taking place over distances of up to 10 nm. With the advent of recent super-resolution methods such as MINFLUX, MINSTED or RASTMIN, th…

Single particle thermometry in bimetallic plasmonic nanostuctures Open

Julián Gargiulo, Matías Herran, Ianina L. Violi, Ana Sousa‐Castillo, Luciana P. Martinez , et al. · 2022

Localized surface plasmons are lossy and generate heat. However, accurate measurement of the temperature of metallic nanoparticles under illumination remains an open challenge, creating difficulties in the interpretation of results across …

Super-resolved FRET imaging by confocal fluorescence-lifetime single-molecule localization microscopy Open

Cecilia Zaza, Germán Chiarelli, Larry S. Zweifel, Mauricio Pilo‐Pais, Evangelos Sisamakis , et al. · 2022

FRET-based approaches are a unique tool for sensing the immediate surroundings and interactions of (bio)molecules. FRET imaging and FLIM (Fluorescence Lifetime Imaging Microscopy) enable the visualization of the spatial distribution of mol…

DNA Self-Assembly of Single Molecules with Deterministic Position and Orientation Open

Aleksandra K. Adamczyk, Teun A.P.M. Huijben, Miguel Sison, Andrea Di Luca, Germán Chiarelli , et al. · 2022

An ideal nanofabrication method should allow the organization of nanoparticles and molecules with nanometric positional precision, stoichiometric control, and well-defined orientation. The DNA origami technique has evolved into a highly ve…

Correction: An alternative to MINFLUX that enables nanometer resolution in a confocal microscope Open

Luciano A. Masullo, Alan M. Szalai, Lucía F. Lopez, Mauricio Pilo‐Pais, Guillermo P. Acuna , et al. · 2022

DNA self-assembly of single molecules with deterministic position and orientation Open

Aleksandra K. Adamczyk, Teun A.P.M. Huijben, Miguel Sison, Andrea Di Luca, Germán Chiarelli , et al. · 2022

An ideal nanofabrication method should allow the organization of nanoparticles and molecules with nanometric positional precision, stoichiometric control and well-defined orientation. The DNA origami technique has evolved into a highly ver…

An alternative to MINFLUX that enables nanometer resolution in a confocal microscope Open

Luciano A. Masullo, Alan M. Szalai, Lucía F. Lopez, Mauricio Pilo‐Pais, Guillermo P. Acuna , et al. · 2022

Unidirectional ultracompact DNA-templated optical antennas Open

Fangjia Zhu, María Sanz‐Paz, Antonio I. Fernández‐Domínguez, Xiaolu Zhuo, Luis M. Liz‐Marzán , et al. · 2022

Optical nanoantennas are structures designed to manipulate light-matter interactions at the nanoscale by interfacing propagating light with localized optical fields. In recent years, a plethora of devices have been realized that are able t…

Multiphoton single-molecule localization by sequential excitation with light minima Open

Luciano A. Masullo, Fernando D. Stefani · 2022

Using sequential excitation with a minimum of light to localize single fluorescent molecules represented a breakthrough because it delivers 1–2 nm precision with moderate photon counts, enabling tracking and super-resolution imaging with t…

PyFocus – A Python package for vectorial calculations of focused optical fields under realistic conditions. Application to toroidal foci Open

Fernando Caprile, Luciano A. Masullo, Fernando D. Stefani · 2022

Challenges on optical printing of colloidal nanoparticles Open

Ianina L. Violi, Luciana P. Martinez, Mariano Barella, Cecilia Zaza, Lukáš Chvátal , et al. · 2021

While colloidal chemistry provides ways to obtain a great variety of nanoparticles with different shapes, sizes, material compositions, and surface functions, their controlled deposition and combination on arbitrary positions of substrates…

Challenges on Optical Printing of Colloidal Nanoparticles Open

Ianina L. Violi, Luciana P. Martinez, Mariano Barella, Cecilia Zaza, Lukáš Chvátal , et al. · 2021

While colloidal chemistry provides ways to obtain a great variety of nanoparticles, with different shapes, sizes, material composition, and surface functions, their controlled deposition and combination on arbitrary positions of substrates…

Fluorescence nanoscopy at the sub-10 nm scale Open

Luciano A. Masullo, Alan M. Szalai, Lucía F. Lopez, Fernando D. Stefani · 2021

A common framework for single-molecule localization using sequential structured illumination Open

Luciano A. Masullo, Lucía F. Lopez, Fernando D. Stefani · 2021

A common framework for single-molecule localization using sequential\n structured illumination Open

Luciano A. Masullo, Lucía F. Lopez, Fernando D. Stefani · 2021

Localization of single fluorescent molecules is key for physicochemical and biophysical measurements such as single-molecule tracking and super-resolution imaging by single-molecule localization microscopy (SMLM). Recently a series of meth…

In Situ Photothermal Response of Single Gold Nanoparticles Through Hyperspectral Imaging AntiStokes Thermometry Open

Mariano Barella, Ianina L. Violi, Julián Gargiulo, Luciana P. Martinez, Florian Goschin , et al. · 2021

Several fields of applications require a reliable characterization of the photothermal response and heat dissipation of nanoscopic systems, which remains a challenging task both for modeling and experimental measurements. Here, we present …

Three-dimensional total-internal reflection fluorescence nanoscopy with nanometric axial resolution by photometric localization of single molecules Open

Alan M. Szalai, Bruno Siarry, Jerónimo Lukin, David J. Williamson, Nicolás Unsain , et al. · 2021

Single-molecule localization microscopy enables far-field imaging with lateral resolution in the range of 10 to 20 nanometres, exploiting the fact that the centre position of a single-molecule’s image can be determined with much higher acc…

<i>In Situ</i> Photothermal Response of\nSingle Gold Nanoparticles\nthrough Hyperspectral Imaging Anti-Stokes Thermometry Open

Mariano Barella, Ianina L. Violi, Julián Gargiulo, Luciana P. Martinez, Florian Goschin , et al. · 2020

Several\nfields of applications require a reliable characterization\nof the photothermal response and heat dissipation of nanoscopic systems,\nwhich remains a challenging task for both modeling and experimental\nmeasurements. Here, we pres…

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