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View article: Effect of age and disease on saliva and serum biomarkers of stress, inflammation, immunity, redox and general health status in pigs
Effect of age and disease on saliva and serum biomarkers of stress, inflammation, immunity, redox and general health status in pigs Open
View article: Changes in Saliva Analytes in Pigs in Different Clinical Situations from Farms Positive to Porcine Reproductive and Respiratory Syndrome (PRRS): A Pilot Study
Changes in Saliva Analytes in Pigs in Different Clinical Situations from Farms Positive to Porcine Reproductive and Respiratory Syndrome (PRRS): A Pilot Study Open
Porcine reproductive and respiratory syndrome (PRRS) is aworldwide spread disease. This study analyzed the changes in saliva analytes of pigs infected with PRRS virus (PRRSV) in different clinical conditions that can appear in PRRSV-positi…
View article: Validation of the Measurement of Beta-Hydroxybutyrate and Non-Esterified Fatty Acids in Bovine Saliva: A Pilot Report
Validation of the Measurement of Beta-Hydroxybutyrate and Non-Esterified Fatty Acids in Bovine Saliva: A Pilot Report Open
Serum beta-hydroxybutyrate (BHB) and non-esterified fatty acids (NEFAs) are biomarkers of situations of negative energetic balance in bovine. However, knowledge about their possible measurement and use in saliva is limited. In this report,…
View article: Use of Saliva Analytes as a Predictive Model to Detect Diseases in the Pig: A Pilot Study
Use of Saliva Analytes as a Predictive Model to Detect Diseases in the Pig: A Pilot Study Open
Background/Objectives: Saliva is gaining importance as a diagnostic sample in pigs. The aim of this research was to evaluate a panel of salivary analytes in three porcine diseases and establish predictive models to detect them. Methods: Sa…
View article: Towards the Measurement of Acute-Phase Proteins in Saliva in Farm Conditions: Development and Validation of a Lateral Flow Assay for the Measurement of C-Reactive Protein in Pigs
Towards the Measurement of Acute-Phase Proteins in Saliva in Farm Conditions: Development and Validation of a Lateral Flow Assay for the Measurement of C-Reactive Protein in Pigs Open
Point-of-care diagnostic tests, such as lateral-flow immunoassay (LFIA), have emerged as a fast diagnostic tool in both human and veterinary medicine. In this paper, a gold nanoparticle-based LFIA device was developed for the measurement o…
View article: New strategies to classify canine pleural effusions and the diagnostic value of acute phase proteins, amylase, and adenosine deaminase in pleural exudates
New strategies to classify canine pleural effusions and the diagnostic value of acute phase proteins, amylase, and adenosine deaminase in pleural exudates Open
Background In dogs, simplified Light's criteria can discriminate transudates from exudates. Other tests used in human medicine are pleural effusion cholesterol (CHOL PE ) and butyrylcholinesterase [BChE PE ], the pleural effusion/serum rat…
View article: Changes in cortisol, cortisone and 11β-hydroxysteroid dehydrogenase type II activity in saliva during pregnancy and lactation in sows
Changes in cortisol, cortisone and 11β-hydroxysteroid dehydrogenase type II activity in saliva during pregnancy and lactation in sows Open
The activity of the enzyme 11β-hydroxysteroid dehydrogenase type II (11β-HSD type II), which can be estimated by the combined measurement of cortisol and cortisone, is gaining importance as a marker for the assessment of stress in pigs. Th…
View article: Measurement of Trace Elements (Zinc, Copper, Magnesium, and Iron) in the Saliva of Horses: Validation Data and Changes in Equine Gastric Ulcer Syndrome (EGUS)
Measurement of Trace Elements (Zinc, Copper, Magnesium, and Iron) in the Saliva of Horses: Validation Data and Changes in Equine Gastric Ulcer Syndrome (EGUS) Open
The objective of this study was to evaluate the possible use of spectrophotometric assays for the measurement of trace elements, including Zinc (Zn), Copper (Cu), Magnesium (Mg), and iron (Fe) in the saliva of horses and study their possib…
View article: Comparison of the Effect of Two Different Handling Conditions at Slaughter in Saliva Analytes in Pigs
Comparison of the Effect of Two Different Handling Conditions at Slaughter in Saliva Analytes in Pigs Open
In this report, different handling conditions at slaughterhouse were studied to assess changes in salivary biomarkers. For this purpose, finishing pigs were divided into two groups, one in which handling was improved to minimize stress (Gr…
View article: Active monitoring of long-eared owl (Asio otus) nestlings reveals widespread exposure to anticoagulant rodenticides across different agricultural landscapes
Active monitoring of long-eared owl (Asio otus) nestlings reveals widespread exposure to anticoagulant rodenticides across different agricultural landscapes Open
The widespread use of anticoagulant rodenticides (ARs) poses a worldwide threat to farmland wildlife. These compounds accumulate in tissues of both target and non-target species, potentially endangering both direct consumers and their pred…
View article: LA SALIVA COMO UNA FUENTE DE BIOMARCADORES EN EL GANADO PORCINO: CONCEPTOS GENERALES Y AVANCES RECIENTES
LA SALIVA COMO UNA FUENTE DE BIOMARCADORES EN EL GANADO PORCINO: CONCEPTOS GENERALES Y AVANCES RECIENTES Open
The use of saliva in the pig has gained importance due in the last years to its ease of sampling, without causing stress or pain in the animal. In addition to identifying pathogens, it is a source of biomarkers used to evaluate general pig…
View article: Saliva Sampling Material Matters: Effects on the Results of Saliva Analysis in Pigs
Saliva Sampling Material Matters: Effects on the Results of Saliva Analysis in Pigs Open
The use of saliva as a biological sample from pigs is of high practical interest because blood collection from pigs is difficult and stressful. In this study, the influence of two different materials, a cotton roll and a polypropylene spon…
View article: Stress responses in pigs postweaning: Effect of heavier hybrid and weaning intact litters
Stress responses in pigs postweaning: Effect of heavier hybrid and weaning intact litters Open
Early and abrupt weaning of pigs is a stressful event. In addition, the use of hyperprolific sows with increasing numbers of live born piglets has decreased the average pig's birth weight, colostrum intake and weaning weight. The aim of th…
View article: Gaining knowledge about biomarkers of the immune system and inflammation in the saliva of pigs: The case of myeloperoxidase, S100A12, and ITIH4
Gaining knowledge about biomarkers of the immune system and inflammation in the saliva of pigs: The case of myeloperoxidase, S100A12, and ITIH4 Open
An assay for the measurement of myeloperoxidase (Mpx) in porcine saliva was developed and validated, and factors influencing Mpx and another two biomarkers of inflammation and immune system, the protein S100A12 and the inter-alpha-trypsin …
View article: Changes in S100A8/A9 and S100A12 and Their Comparison with Other Analytes in the Saliva of Pigs with Diarrhea Due to E. coli
Changes in S100A8/A9 and S100A12 and Their Comparison with Other Analytes in the Saliva of Pigs with Diarrhea Due to E. coli Open
The family of calgranulins includes S100A8 (calgranulin A), S100A9 (calgranulin B), which can appear as a heterodimer known as S100A8/A9 or calprotectin, and S100A12 (calgranulin C). These proteins are related to different inflammatory con…
View article: Changes in salivary biomarkers of stress, inflammation, redox status, and muscle damage due to Streptococcus suis infection in pigs
Changes in salivary biomarkers of stress, inflammation, redox status, and muscle damage due to Streptococcus suis infection in pigs Open
View article: Divergences between serum C-reactive protein and ferritin concentrations in canine pyometra
Divergences between serum C-reactive protein and ferritin concentrations in canine pyometra Open
The main aim of this report was to investigate and compare the response of serum C-reactive protein (CRP) and ferritin, two positive acute phase proteins (APPs) which usually show an increase in inflammatory processes, in dogs with pyometr…
View article: S-100 Proteins: Basics and Applications as Biomarkers in Animals with Special Focus on Calgranulins (S100A8, A9, and A12)
S-100 Proteins: Basics and Applications as Biomarkers in Animals with Special Focus on Calgranulins (S100A8, A9, and A12) Open
S100 proteins are a group of calcium-binding proteins which received this name because of their solubility in a 100% saturated solution of ammonium sulphate. They have a similar molecular mass of 10–12 KDa and share 25–65% similarity in th…
View article: Changes in Calprotectin (S100A8-A9) and Aldolase in the Saliva of Horses with Equine Gastric Ulcer Syndrome
Changes in Calprotectin (S100A8-A9) and Aldolase in the Saliva of Horses with Equine Gastric Ulcer Syndrome Open
Equine gastric ulcer syndrome (EGUS) is a highly prevalent disease that affects horses worldwide. Within EGUS, two different forms have been described: equine squamous gastric disease (ESGD) and equine glandular gastric disease (EGGD). The…
View article: Measurement of Calprotectin (S100A8/A9) in the Saliva of Pigs: Validation Data of A Commercially Available Automated Assay and Changes in Sepsis, Inflammation, and Stress
Measurement of Calprotectin (S100A8/A9) in the Saliva of Pigs: Validation Data of A Commercially Available Automated Assay and Changes in Sepsis, Inflammation, and Stress Open
Calprotectin (CALP, S100A8/A9), also named myeloid-related protein 8/14, is a dimer complex of S100A8 and S100A9 that belongs to the S-100 protein family. It is involved in inflammation and has a wide range of proinflammatory functions, su…
View article: Trace Elements and Ferritin in Pig Saliva: Variations during Fattening, Time of Sampling, Effect of Dirtiness and Stability under Different Storage Conditions
Trace Elements and Ferritin in Pig Saliva: Variations during Fattening, Time of Sampling, Effect of Dirtiness and Stability under Different Storage Conditions Open
The objective of this study was to evaluate the possible changes of zinc (Zn), copper (Cu), iron (Fe) and ferritin during the entire productive cycle in fattening pigs and at different diurnal sampling times. Moreover, the possible effects…
View article: Different Types of Glucocorticoids to Evaluate Stress and Welfare in Animals and Humans: General Concepts and Examples of Combined Use
Different Types of Glucocorticoids to Evaluate Stress and Welfare in Animals and Humans: General Concepts and Examples of Combined Use Open
The main glucocorticoids involved in the stress response are cortisol and cortisone in most mammals and corticosterone in birds and rodents. Therefore, these analytes are currently the biomarkers more frequently used to evaluate the physio…
View article: Postweaning Behaviour and Stress Indicators in Pigs: Effect of Heavier Hybrid and Weaning Intact Litters in Farrowing Pens for Loose-Housed Sows
Postweaning Behaviour and Stress Indicators in Pigs: Effect of Heavier Hybrid and Weaning Intact Litters in Farrowing Pens for Loose-Housed Sows Open
View article: Evaluation of a Comprehensive Profile of Salivary Analytes for the Diagnosis of the Equine Gastric Ulcer Syndrome
Evaluation of a Comprehensive Profile of Salivary Analytes for the Diagnosis of the Equine Gastric Ulcer Syndrome Open
In this report, the measurement of salivary biomarkers as an aid for diagnosis of equine gastric ulcer syndrome (EGUS) was studied. A comprehensive panel of 23 salivary analytes was measured in the saliva of horses affected by EGUS and com…
View article: Automated assays for trace elements and ferritin measurement in saliva of pigs: Analytical validation and a pilot application to evaluate different iron status
Automated assays for trace elements and ferritin measurement in saliva of pigs: Analytical validation and a pilot application to evaluate different iron status Open
The aim of this study was to validate automated methods to measure iron (Fe), zinc (Zn), copper (Cu) and ferritin in pig saliva samples. A complete analytical validation was performed of all assays. In addition, these methods were applied …
View article: Effects of pen faeces and feed contamination in biomarkers determination in oral fluid of pigs
Effects of pen faeces and feed contamination in biomarkers determination in oral fluid of pigs Open
View article: Evaluation of the Effect of Sampling Time on Biomarkers of Stress, Immune System, Redox Status and Other Biochemistry Analytes in Saliva of Finishing Pigs
Evaluation of the Effect of Sampling Time on Biomarkers of Stress, Immune System, Redox Status and Other Biochemistry Analytes in Saliva of Finishing Pigs Open
This study aims to evaluate the possible variations due to the sampling time in the day in 26 analytes of pigs’ saliva, related to stress, the immune system, redox status and other biomarkers related to metabolism and selected tissues and …
View article: Changes in a Comprehensive Profile of Saliva Analytes in Fattening Pigs during a Complete Productive Cycle: A Longitudinal Study
Changes in a Comprehensive Profile of Saliva Analytes in Fattening Pigs during a Complete Productive Cycle: A Longitudinal Study Open
A comprehensive panel of 29 salivary analytes was measured in fattening pigs to evaluate its possible changes along their productive cycle. The identification of those changes would allow a better interpretation of the results according to…
View article: Measurement of anti SARS-CoV-2 RBD IgG in saliva: validation of a highly sensitive assay and effects of the sampling collection method and correction by protein
Measurement of anti SARS-CoV-2 RBD IgG in saliva: validation of a highly sensitive assay and effects of the sampling collection method and correction by protein Open
Objectives To develop and evaluate a new highly sensitive assay to detect IgG anti-SARS-CoV-2 RBD in saliva samples. Methods A two-step sandwich type immunoassay based on the amplified luminescent proximity homogeneous technology was devel…
View article: Effect of thermal and chemical treatments used for SARS-COV-2 inactivation in the measurement of saliva analytes
Effect of thermal and chemical treatments used for SARS-COV-2 inactivation in the measurement of saliva analytes Open
The present study aims to assess the effects of thermal and chemical inactivating procedures, that can be used for SARS-CoV-2 inactivation, on different salivary analytes. SDS–Polyacrylamide Gel Electrophoresis (SDS-PAGE) protein profile a…