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View article: Pathway-selective strategies reveal specific PI3K–mTOR inhibitors as key partners in KRAS G12D-targeted therapy
Pathway-selective strategies reveal specific PI3K–mTOR inhibitors as key partners in KRAS G12D-targeted therapy Open
KRAS G12D is the most common oncogenic mutation in pancreatic ductal adenocarcinoma (PDAC), driving resistance and heterogeneity. Using robotic high-throughput drug screening with live-cell imaging, we evaluated normalized organoid growth …
View article: HIF isoforms contribute distinctly during human NK cell activation
HIF isoforms contribute distinctly during human NK cell activation Open
Hypoxia-inducible factors (HIFs) are critical transcriptional regulators that enable cellular adaptation to low oxygen environments. Natural killer (NK) cells are key effectors of innate immunity which frequently operate in hypoxic tissues…
View article: <i>DRP1</i>depletion protects NK cells against hypoxia-induced dysfunction
<i>DRP1</i>depletion protects NK cells against hypoxia-induced dysfunction Open
Hypoxia within the tumor microenvironment poses a major barrier to the efficacy of NK cell-based immunotherapies for solid tumors. In this study, we investigated the influence of hypoxia on NK cell function and mitochondria. We found that …
View article: Co-targeting of VEGFR2 and PD-L1 promotes survival and vasculature normalization in pleural mesothelioma
Co-targeting of VEGFR2 and PD-L1 promotes survival and vasculature normalization in pleural mesothelioma Open
Pleural mesothelioma (PM) is an aggressive cancer caused by asbestos exposure, with limited treatment options and poor prognosis, highlighting the need for more effective therapies. Combining immune checkpoint blockade with anti-angiogenic…
View article: A Minimally Invasive Transthoracic Injection Technique for Reproducible Intrapleural Delivery in Mice
A Minimally Invasive Transthoracic Injection Technique for Reproducible Intrapleural Delivery in Mice Open
The development of standardised, reproducible preclinical models is essential for advancing pleural mesothelioma (PM) research. Here, we present a simple and reliable minimally invasive transthoracic intrapleural injection technique that c…
View article: Development and validation of the Normalized Organoid Growth Rate (NOGR) metric in brightfield imaging-based assays
Development and validation of the Normalized Organoid Growth Rate (NOGR) metric in brightfield imaging-based assays Open
This study focuses on refining growth-rate-based drug response metrics for patient-derived tumor organoid screening using brightfield live-cell imaging. Traditional metrics like Normalized Growth Rate Inhibition (GR) and Normalized Drug Re…
View article: Auranofin repurposing for lung and pancreatic cancer: low CA12 expression as a marker of sensitivity in patient-derived organoids, with potentiated efficacy by AKT inhibition
Auranofin repurposing for lung and pancreatic cancer: low CA12 expression as a marker of sensitivity in patient-derived organoids, with potentiated efficacy by AKT inhibition Open
Background This study explores the repurposing of Auranofin (AF), an anti-rheumatic drug, for treating non-small cell lung cancer (NSCLC) adenocarcinoma and pancreatic ductal adenocarcinoma (PDAC). Drug repurposing in oncology offers a cos…
View article: IL-15-secreting CAR natural killer cells directed toward the pan-cancer target CD70 eliminate both cancer cells and cancer-associated fibroblasts
IL-15-secreting CAR natural killer cells directed toward the pan-cancer target CD70 eliminate both cancer cells and cancer-associated fibroblasts Open
View article: Correction: Krayem et al. The Benefit of Reactivating p53 under MAPK Inhibition on the Efficacy of Radiotherapy in Melanoma. Cancers 2019, 11, 1093
Correction: Krayem et al. The Benefit of Reactivating p53 under MAPK Inhibition on the Efficacy of Radiotherapy in Melanoma. Cancers 2019, 11, 1093 Open
In the original article [...]
View article: Single-organoid analysis reveals clinically relevant treatment-resistant and invasive subclones in pancreatic cancer
Single-organoid analysis reveals clinically relevant treatment-resistant and invasive subclones in pancreatic cancer Open
Pancreatic ductal adenocarcinoma (PDAC) is one of the most lethal diseases, characterized by a treatment-resistant and invasive nature. In line with these inherent aggressive characteristics, only a subset of patients shows a clinical resp…
View article: Protecting public health and the environment: towards a general ban on cellulose acetate cigarette filters in the European Union
Protecting public health and the environment: towards a general ban on cellulose acetate cigarette filters in the European Union Open
After the establishment of a causal relationship between tobacco use and cancer in the 1950s, cellulose acetate filters were introduced with the claim to reduce the adverse health impact of unfiltered cigarettes. Often perceived to be more…
View article: Establishment of head and neck squamous cell carcinoma mouse models for cetuximab resistance and sensitivity
Establishment of head and neck squamous cell carcinoma mouse models for cetuximab resistance and sensitivity Open
Aim: Acquired resistance to the targeted agent cetuximab poses a significant challenge in finding effective anti-cancer treatments for head and neck squamous cell carcinoma (HNSCC). To accurately study novel combination treatments, suitabl…
View article: Phototoxicity and cell passage affect intracellular reactive oxygen species levels and sensitivity towards non-thermal plasma treatment in fluorescently-labeled cancer cells
Phototoxicity and cell passage affect intracellular reactive oxygen species levels and sensitivity towards non-thermal plasma treatment in fluorescently-labeled cancer cells Open
Live-cell imaging with fluorescence microscopy is a powerful tool, especially in cancer research, widely-used for capturing dynamic cellular processes over time. However, light-induced toxicity (phototoxicity) can be incurred from this met…
View article: Targeting CD70 in combination with chemotherapy to enhance the anti-tumor immune effects in non-small cell lung cancer
Targeting CD70 in combination with chemotherapy to enhance the anti-tumor immune effects in non-small cell lung cancer Open
Despite the recent emergence of immune checkpoint inhibitors, clinical outcomes of metastatic NSCLC patients remain poor, pointing out the unmet need to develop novel therapies to enhance the anti-tumor immune response in NSCLC. In this re…
View article: Phototoxicity and Cell Passage Affect Intracellular Reactive Oxygen Species Levels and Sensitivity Towards Non-Thermal Plasma Treatment in Fluorescently-Labeled Cancer Cells
Phototoxicity and Cell Passage Affect Intracellular Reactive Oxygen Species Levels and Sensitivity Towards Non-Thermal Plasma Treatment in Fluorescently-Labeled Cancer Cells Open
Raw data for the manuscript "Phototoxicity and Cell Passage Affect Intracellular Reactive Oxygen Species Levels and Sensitivity Towards Non-Thermal Plasma Treatment in Fluorescently-Labeled Cancer Cells".
View article: Phototoxicity and Cell Passage Affect Intracellular Reactive Oxygen Species Levels and Sensitivity Towards Non-Thermal Plasma Treatment in Fluorescently-Labeled Cancer Cells
Phototoxicity and Cell Passage Affect Intracellular Reactive Oxygen Species Levels and Sensitivity Towards Non-Thermal Plasma Treatment in Fluorescently-Labeled Cancer Cells Open
Raw data for the manuscript "Phototoxicity and Cell Passage Affect Intracellular Reactive Oxygen Species Levels and Sensitivity Towards Non-Thermal Plasma Treatment in Fluorescently-Labeled Cancer Cells".
View article: Auranofin Synergizes with the PARP Inhibitor Olaparib to Induce ROS-Mediated Cell Death in Mutant p53 Cancers
Auranofin Synergizes with the PARP Inhibitor Olaparib to Induce ROS-Mediated Cell Death in Mutant p53 Cancers Open
Auranofin (AF) is a potent, off-patent thioredoxin reductase (TrxR) inhibitor that efficiently targets cancer via reactive oxygen species (ROS)- and DNA damage-mediated cell death. The goal of this study is to enhance the efficacy of AF as…
View article: Uncovering the hidden threat: single-organoid analysis reveals clinically relevant treatment-resistant and invasive subclones in pancreatic cancer
Uncovering the hidden threat: single-organoid analysis reveals clinically relevant treatment-resistant and invasive subclones in pancreatic cancer Open
Pancreatic ductal adenocarcinoma (PDAC) is one of the most lethal diseases, characterized by a treatment-resistant and invasive nature. In-line with these inherent aggressive characteristics, only a subset of patients show a clinical respo…
View article: Cellular senescence in cancer: clinical detection and prognostic implications
Cellular senescence in cancer: clinical detection and prognostic implications Open
Cellular senescence is a state of stable cell-cycle arrest with secretory features in response to cellular stress. Historically, it has been considered as an endogenous evolutionary homeostatic mechanism to eliminate damaged cells, includi…
View article: Multiparametric Tumor Organoid Drug Screening Using Widefield Live-Cell Imaging for Bulk and Single-Organoid Analysis
Multiparametric Tumor Organoid Drug Screening Using Widefield Live-Cell Imaging for Bulk and Single-Organoid Analysis Open
Patient-derived tumor organoids (PDTOs) hold great promise for preclinical and translational research and predicting the patient therapy response from ex vivo drug screenings. However, current adenosine triphosphate (ATP)-based drug screen…
View article: OrBITS: label-free and time-lapse monitoring of patient derived organoids for advanced drug screening
OrBITS: label-free and time-lapse monitoring of patient derived organoids for advanced drug screening Open
View article: Co-Targeting the EGFR and PI3K/Akt Pathway to Overcome Therapeutic Resistance in Head and Neck Squamous Cell Carcinoma: What about Autophagy?
Co-Targeting the EGFR and PI3K/Akt Pathway to Overcome Therapeutic Resistance in Head and Neck Squamous Cell Carcinoma: What about Autophagy? Open
Resistance to EGFR-targeted therapy is a major obstacle on the road to effective treatment options for head and neck cancers. During the search for underlying mechanisms and regulators of this resistance, there were several indications tha…
View article: Optimization of the Solvent and In Vivo Administration Route of Auranofin in a Syngeneic Non-Small Cell Lung Cancer and Glioblastoma Mouse Model
Optimization of the Solvent and In Vivo Administration Route of Auranofin in a Syngeneic Non-Small Cell Lung Cancer and Glioblastoma Mouse Model Open
The antineoplastic activity of the thioredoxin reductase 1 (TrxR) inhibitor, auranofin (AF), has already been investigated in various cancer mouse models as a single drug, or in combination with other molecules. However, there are inconsis…
View article: The prognostic impact of the immune signature in head and neck squamous cell carcinoma
The prognostic impact of the immune signature in head and neck squamous cell carcinoma Open
Head and neck squamous cell carcinoma (HNSCC) is a heterogeneous group of tumors that retain their poor prognosis despite recent advances in their standard of care. As the involvement of the immune system against HNSCC development is well-…
View article: Expression of SARS-CoV-2-Related Surface Proteins in Non-Small-Cell Lung Cancer Patients and the Influence of Standard of Care Therapy
Expression of SARS-CoV-2-Related Surface Proteins in Non-Small-Cell Lung Cancer Patients and the Influence of Standard of Care Therapy Open
In this study, we aimed to study the expression of SARS-CoV-2-related surface proteins in non-small-cell lung cancer (NSCLC) cells and identify clinicopathological characteristics that are related to increased membranous (m)ACE2 protein ex…
View article: Prognostic implications of cellular senescence in resected non-small cell lung cancer
Prognostic implications of cellular senescence in resected non-small cell lung cancer Open
The presence of a tumoral SS particularly based on high p16INK4a expression significantly affects OS in NSCLC adenocarcinoma. In this light, adjuvant senolytic therapy could be an interesting strategy for NSCLC patients harborin…
View article: NTRK Gene Fusion Detection in a Pan-Cancer Setting Using the Idylla GeneFusion Assay
NTRK Gene Fusion Detection in a Pan-Cancer Setting Using the Idylla GeneFusion Assay Open
Recently, approval of tyrosine receptor kinase (TRK) inhibitors by Food and Drug Administration and European Medicines Agency in NTRK fusion-positive cancer types has led to a variety of proposed testing algorithms. In this study, performa…
View article: The effect of local <scp>non‐thermal</scp> plasma therapy on the <scp>cancer‐immunity</scp> cycle in a melanoma mouse model
The effect of local <span>non‐thermal</span> plasma therapy on the <span>cancer‐immunity</span> cycle in a melanoma mouse model Open
Melanoma remains a deadly cancer despite significant advances in immune checkpoint blockade and targeted therapies. The incidence of melanoma is also growing worldwide, which highlights the need for novel treatment options and strategic co…
View article: The CD70-CD27 axis in oncology: the new kids on the block
The CD70-CD27 axis in oncology: the new kids on the block Open
View article: Expression of SARS-CoV-2 Related Surface Proteins in Non-Small Cell Lung Cancer Patients and the Influence of Standard of Care Therapy
Expression of SARS-CoV-2 Related Surface Proteins in Non-Small Cell Lung Cancer Patients and the Influence of Standard of Care Therapy Open