Filip Mihalič
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View article: Defining short linear motif binding determinants by phage display‐based deep mutational scanning
Defining short linear motif binding determinants by phage display‐based deep mutational scanning Open
Deep mutational scanning (DMS) has emerged as a powerful approach for evaluating the effects of mutations on binding or function. Here, we developed a DMS by phage display protocol to define the specificity determinants of short linear mot…
View article: Defining short linear motif binding determinants by phage-based multiplexed deep mutational scanning
Defining short linear motif binding determinants by phage-based multiplexed deep mutational scanning Open
Deep mutational scanning (DMS) has emerged as a powerful approach for evaluating the effects of mutations on binding or function. Here, we developed a multiplexed DMS by phage display protocol to define the binding determinants of short li…
View article: The evolutionary and molecular history of a chikungunya virus outbreak lineage
The evolutionary and molecular history of a chikungunya virus outbreak lineage Open
In 2018–2019, Thailand experienced a nationwide spread of chikungunya virus (CHIKV), with approximately 15,000 confirmed cases of disease reported. Here, we investigated the evolutionary and molecular history of the East/Central/South Afri…
View article: The evolutionary and molecular history of a chikungunya virus outbreak lineage
The evolutionary and molecular history of a chikungunya virus outbreak lineage Open
In 2018–2019, Thailand experienced a nationwide spread of chikungunya virus (CHIKV), with approximately 15,000 confirmed cases of disease reported. Here, we investigated the evolutionary and molecular history of the East/Central/South Afri…
View article: Conservation of Affinity Rather Than Sequence Underlies a Dynamic Evolution of the Motif-Mediated p53/MDM2 Interaction in Ray-Finned Fishes
Conservation of Affinity Rather Than Sequence Underlies a Dynamic Evolution of the Motif-Mediated p53/MDM2 Interaction in Ray-Finned Fishes Open
The transcription factor and cell cycle regulator p53 is marked for degradation by the ubiquitin ligase MDM2. The interaction between these 2 proteins is mediated by a conserved binding motif in the disordered p53 transactivation domain (p…
View article: Identification of motif-based interactions between SARS-CoV-2 protein domains and human peptide ligands pinpoint antiviral targets
Identification of motif-based interactions between SARS-CoV-2 protein domains and human peptide ligands pinpoint antiviral targets Open
View article: Conservation of affinity rather than sequence underlies a dynamic evolution of the motif-mediated p53/MDM2 interaction in teleosts
Conservation of affinity rather than sequence underlies a dynamic evolution of the motif-mediated p53/MDM2 interaction in teleosts Open
The transcription factor and cell cycle regulator p53 is marked for degradation by the ubiquitin ligase MDM2. The interaction between these two proteins is mediated by a conserved binding motif in the disordered p53 transactivation domain …
View article: Gustavson syndrome is caused by an in-frame deletion in RBMX associated with potentially disturbed SH3 domain interactions
Gustavson syndrome is caused by an in-frame deletion in RBMX associated with potentially disturbed SH3 domain interactions Open
View article: Evolution of affinity between p53 transactivation domain and <scp>MDM2</scp> across the animal kingdom demonstrates high plasticity of motif‐mediated interactions
Evolution of affinity between p53 transactivation domain and <span>MDM2</span> across the animal kingdom demonstrates high plasticity of motif‐mediated interactions Open
The interaction between the transcription factor p53 and the ubiquitin ligase MDM2 results in the degradation of p53 and is well‐studied in cancer biology and drug development. Available sequence data suggest that both p53 and MDM2‐family …
View article: Large-scale phage-based screening reveals extensive pan-viral mimicry of host short linear motifs
Large-scale phage-based screening reveals extensive pan-viral mimicry of host short linear motifs Open
View article: Evolution of affinity between p53 and MDM2 across the animal kingdom demonstrates high plasticity of motif-mediated interactions
Evolution of affinity between p53 and MDM2 across the animal kingdom demonstrates high plasticity of motif-mediated interactions Open
The interaction between the transcription factor p53 and the ubiquitin ligase MDM2 results in degradation of p53 and is well studied in cancer biology and drug development. Available sequence data suggest that both p53 and MDM2-family prot…
View article: Evaluation of affinity-purification coupled to mass spectrometry approaches for capture of short linear motif-based interactions
Evaluation of affinity-purification coupled to mass spectrometry approaches for capture of short linear motif-based interactions Open
Low affinity and transient protein-protein interactions, such as short linear motif (SLiM)-based interactions, require dedicated experimental tools for discovery and validation. Here, we evaluated and compared biotinylated peptide pulldown…
View article: Evaluation of affinity-purification coupled to mass spectrometry approaches for capture of short linear motif-based interactions
Evaluation of affinity-purification coupled to mass spectrometry approaches for capture of short linear motif-based interactions Open
Low affinity and transient protein-protein interactions, such as short linear motif (SLiM)-based interactions, require dedicated experimental tools for discovery and validation. Here, we evaluated and compared biotinylated peptide pulldown…
View article: Identification of motif-based interactions between SARS-CoV-2 protein domains and human peptide ligands pinpoint antiviral targets
Identification of motif-based interactions between SARS-CoV-2 protein domains and human peptide ligands pinpoint antiviral targets Open
The infection and replication cycle of all viruses depend on interactions between viral and host proteins. Each of these protein-protein interactions is therefore a potential drug target. These host-virus interactions often involve a disor…
View article: Monoclonal antibodies binding data for SARS-CoV-2 proteins
Monoclonal antibodies binding data for SARS-CoV-2 proteins Open
View article: Large-scale phage-based screening reveals extensive pan-viral mimicry of host short linear motifs
Large-scale phage-based screening reveals extensive pan-viral mimicry of host short linear motifs Open
SUMMARY Viruses mimic host short linear motifs (SLiMs) to hijack and deregulate cellular functions. Studies of motif-mediated interactions therefore provide insight into virus-host dependencies, and reveal targets for therapeutic intervent…
View article: Extensive pan-viral mimicry of host short linear motifs hijack of the human interactome
Extensive pan-viral mimicry of host short linear motifs hijack of the human interactome Open
Code used for generating Figure 2D,E,F and supp Figure 2,3,4
View article: Development of Monoclonal Antibodies to Detect for SARS-CoV-2 Proteins
Development of Monoclonal Antibodies to Detect for SARS-CoV-2 Proteins Open
View article: Proteome‐scale mapping of binding sites in the unstructured regions of the human proteome
Proteome‐scale mapping of binding sites in the unstructured regions of the human proteome Open
View article: Large scale discovery of coronavirus-host factor protein interaction motifs reveals SARS-CoV-2 specific mechanisms and vulnerabilities
Large scale discovery of coronavirus-host factor protein interaction motifs reveals SARS-CoV-2 specific mechanisms and vulnerabilities Open
View article: Large scale discovery of coronavirus-host factor protein interaction motifs reveals SARS-CoV-2 specific mechanisms and vulnerabilities
Large scale discovery of coronavirus-host factor protein interaction motifs reveals SARS-CoV-2 specific mechanisms and vulnerabilities Open
Viral proteins make extensive use of short peptide interaction motifs to hijack cellular host factors. However, current methods do not identify this important class of protein-protein interactions. Uncovering peptide mediated interactions …
View article: Proteome-scale amino-acid resolution footprinting of protein-binding sites in the intrinsically disordered regions of the human proteome
Proteome-scale amino-acid resolution footprinting of protein-binding sites in the intrinsically disordered regions of the human proteome Open
Specific protein-protein interactions are central to all processes that underlie cell physiology. Numerous studies using a wide range of experimental approaches have identified tens of thousands of human protein-protein interactions. Howev…
View article: Discovery of an Allosteric Ligand Binding Site in SMYD3 Lysine Methyltransferase
Discovery of an Allosteric Ligand Binding Site in SMYD3 Lysine Methyltransferase Open
SMYD3 is a multifunctional epigenetic enzyme with lysine methyltransferase activity and various interaction partners. It is implicated in the pathophysiology of cancers but with an unclear mechanism. To discover tool compounds for clarifyi…