Fiona Constable
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View article: First Report of Hoya tobamovirus 2 in <i>Hoya</i> Species in Australia
First Report of Hoya tobamovirus 2 in <i>Hoya</i> Species in Australia Open
View article: Grapevine red blotch virus (GRBV) in a historical germplasm collection in south-eastern Australia
Grapevine red blotch virus (GRBV) in a historical germplasm collection in south-eastern Australia Open
The first detection of grapevine red blotch virus (syn. GRBV; species Grablovirus vitis, genus Grablovirus, family Geminiviridae) in Australia was initially reported in several grapevine varieties in Western Australia in 2022, but its impa…
View article: Summary of taxonomy changes ratified by the International Committee on Taxonomy of Viruses from the Plant Viruses Subcommittee, 2025
Summary of taxonomy changes ratified by the International Committee on Taxonomy of Viruses from the Plant Viruses Subcommittee, 2025 Open
In March 2025, following the annual International Committee on Taxonomy of Viruses (ICTV) ratification vote, newly proposed taxa were added to those under the mandate of the Plant Viruses Subcommittee. In brief, 1 new order, 3 new families…
View article: First detection and tracing of grapevine red blotch virus (GRBV) in Australia using tiled amplicon sequencing
First detection and tracing of grapevine red blotch virus (GRBV) in Australia using tiled amplicon sequencing Open
View article: Development and validation of X-ComEC qPCR, a novel assay for accurate universal detection of both Xylella fastidiosa and Xylella taiwanensis
Development and validation of X-ComEC qPCR, a novel assay for accurate universal detection of both Xylella fastidiosa and Xylella taiwanensis Open
Xylella fastidiosa is a devastating plant pathogenic bacteria known for its broad host range, in contrast to the related species Xylella taiwanensis , which is only known to cause disease in Asian pears. Despite the potential threats they …
View article: A list of eclectic viruses, virus-like diseases and viroids of grapevines that should not be considered for regulatory oversight: a global plea from virologists
A list of eclectic viruses, virus-like diseases and viroids of grapevines that should not be considered for regulatory oversight: a global plea from virologists Open
Grapevine ( Vitis spp.) propagation material is profusely exchanged across geographic and regulatory boundaries. Trading germplasm increases the diversity of cultivars and rootstocks with enhanced viticultural attributes but also risks the…
View article: First record of a new Frankliniella occidentalis (Pergande, 1895) (Thysanoptera: Thripidae) genetic lineage in Australia
First record of a new Frankliniella occidentalis (Pergande, 1895) (Thysanoptera: Thripidae) genetic lineage in Australia Open
View article: 'Candidatus Phytoplasma vignae’, assigning a species description to a long-known phytoplasma occurring in northern Australia
'Candidatus Phytoplasma vignae’, assigning a species description to a long-known phytoplasma occurring in northern Australia Open
Gene- and genome-based approaches were used to determine whether Vigna little leaf (ViLL) phytoplasma, which occurs in northern Australia, is a distinct ‘ Candidatus Phytoplasma’ species. The ViLL 16S rRNA gene sequences exhibited the high…
View article: All‐in‐one <i>Xylella</i> detection and identification: A nanopore sequencing‐compatible conventional PCR
All‐in‐one <i>Xylella</i> detection and identification: A nanopore sequencing‐compatible conventional PCR Open
Xylella fastidiosa is a plant‐pathogenic bacterium that poses a serious threat to the production of economically important plant species including grapes, almonds, olives and a broad range of amenity plants, causing significant economic lo…
View article: A metagenomic investigation of phytoplasma diversity in Australian vegetable growing regions
A metagenomic investigation of phytoplasma diversity in Australian vegetable growing regions Open
In this study, metagenomic sequence data was used to investigate the phytoplasma taxonomic diversity in vegetable-growing regions across Australia. Metagenomic sequencing was performed on 195 phytoplasma-positive samples, originating eithe…
View article: The Australian Biosecurity Genomic Database: a new resource for high-throughput sequencing analysis based on the National Notifiable Disease List of Terrestrial Animals
The Australian Biosecurity Genomic Database: a new resource for high-throughput sequencing analysis based on the National Notifiable Disease List of Terrestrial Animals Open
The Australian Biosecurity Genomic Database (ABGD) is a curated collection of reference viral genome sequences based on the Australian National Notifiable Disease List of Terrestrial Animals. It was created to facilitate the screening of h…
View article: Genetic Diversity of Grapevine Virus A in Three Australian Vineyards Using Amplicon High Throughput Sequencing (Amplicon-HTS)
Genetic Diversity of Grapevine Virus A in Three Australian Vineyards Using Amplicon High Throughput Sequencing (Amplicon-HTS) Open
Shiraz disease (SD) is one of the most destructive viral diseases of grapevines in Australia and is known to cause significant economic loss to local growers. Grapevine virus A (GVA) was reported to be the key pathogen associated with this…
View article: High-quality full genome assembly of historic <i>Xylella fastidiosa</i> strains from ICMP collection using a hybrid sequencing approach
High-quality full genome assembly of historic <i>Xylella fastidiosa</i> strains from ICMP collection using a hybrid sequencing approach Open
High-quality complete genomes of five Xylella fastidiosa strains were assembled by combining Nanopore and Illumina sequencing data. Among these, International Collection of Micro-organisms from Plants (ICMP) 8731, ICMP 8742 and ICMP 8745 b…
View article: The genetic variability of grapevine Pinot gris virus (GPGV) in Australia
The genetic variability of grapevine Pinot gris virus (GPGV) in Australia Open
View article: The observation of taxonomic boundaries for the 16SrII and 16SrXXV phytoplasmas using genome-based delimitation
The observation of taxonomic boundaries for the 16SrII and 16SrXXV phytoplasmas using genome-based delimitation Open
Within the 16SrII phytoplasma group, subgroups A–X have been classified based on restriction fragment length polymorphism of their 16S rRNA gene, and two species have been described, namely ‘ Candidatus Phytoplasma aurantifolia’ and ‘ Ca .…
View article: First detection of five previously unreported grapevine viruses in Australia
First detection of five previously unreported grapevine viruses in Australia Open
Five grapevine viruses, including grapevine geminivirus A (GGVA), grapevine Syrah virus 1 (GSyV-1), grapevine Cabernet Sauvignon reovirus (GCSV), grapevine virus F (GVF) and grapevine Red Globe virus (GRGV) have been detected in four South…
View article: The genetic variability of grapevine Pinot gris virus (GPGV) in Australia
The genetic variability of grapevine Pinot gris virus (GPGV) in Australia Open
Grapevine Pinot gris virus (GPGV; genus Trichovirus in the family Betaflexiviridae ) was found in Australia in 2016, but the impact on the production of nursery material and fruit in Australia is unknown. This study investigated the preval…
View article: A Chronological Study on Grapevine Leafroll-Associated Virus 2 in Australia
A Chronological Study on Grapevine Leafroll-Associated Virus 2 in Australia Open
Grapevine leafroll disease affects the health status of grapevines worldwide. Most studies in Australia have focused on grapevine leafroll-associated viruses 1 and 3, while little attention has been given to other leafroll virus types, in …
View article: First report of <i>Grapevine rubodvirus 2</i> infecting <i>Vitis vinifera</i> cv. Malbec in Australia
First report of <i>Grapevine rubodvirus 2</i> infecting <i>Vitis vinifera</i> cv. Malbec in Australia Open
Grapevine rubodvirus 2 (syn. Grapevine Muscat rose virus; GMRV, genus Rubodvirus, family Phenuiviridae) is a negative-strand RNA virus with a tri-segmented genome (Diaz-Lara et al., 2019). It has only been described in a symptomless grapev…
View article: Prevalences of Tobamovirus Contamination in Seed Lots of Tomato and Capsicum
Prevalences of Tobamovirus Contamination in Seed Lots of Tomato and Capsicum Open
Seed lots of tomato and capsicum (Solanum lycopersicon and Capsicum annuum, respectively) are required to be free of quarantine pests before their entry to Australia is permitted. Testing of samples from 118 larger seed lots in the period …
View article: A Metagenomic Investigation of the Viruses Associated with Shiraz Disease in Australia
A Metagenomic Investigation of the Viruses Associated with Shiraz Disease in Australia Open
Shiraz disease (SD) is an economically important virus-associated disease that can significantly reduce yield in sensitive grapevine varieties and has so far only been reported in South Africa and Australia. In this study, RT-PCR and metag…
View article: Genome Characterisation of the CGMMV Virus Population in Australia—Informing Plant Biosecurity Policy
Genome Characterisation of the CGMMV Virus Population in Australia—Informing Plant Biosecurity Policy Open
The detection of cucumber green mottle mosaic (CGMMV) in the Northern Territory (NT), Australia, in 2014 led to the introduction of strict quarantine measures for the importation of cucurbit seeds by the Australian federal government. Furt…
View article: Ability of Non-Hosts and Cucurbitaceous Weeds to Transmit Cucumber Green Mottle Mosaic Virus
Ability of Non-Hosts and Cucurbitaceous Weeds to Transmit Cucumber Green Mottle Mosaic Virus Open
Cucumber green mottle mosaic virus (CGMMV) is a Tobamovirus of economic importance affecting cucurbit crops and Asian cucurbit vegetables. Non-host crops of CGMMV, including capsicum (Capsicum annum), sweetcorn (Zea mays), and okra (Abelmo…
View article: First report of watermelon crinkle leaf associated virus-1 (WCLaV-1) in watermelon (Citrullus lanatus) in Australia
First report of watermelon crinkle leaf associated virus-1 (WCLaV-1) in watermelon (Citrullus lanatus) in Australia Open
Surveys of key cucurbit production regions in New South Wales, Australia were undertaken to catalogue the diversity of viral pathogens affecting field grown cropping systems. Analysis of the collected isolates confirmed a detection of wate…
View article: Targeted Whole Genome Sequencing (TWG-Seq) of Cucumber Green Mottle Mosaic Virus Using Tiled Amplicon Multiplex PCR and Nanopore Sequencing
Targeted Whole Genome Sequencing (TWG-Seq) of Cucumber Green Mottle Mosaic Virus Using Tiled Amplicon Multiplex PCR and Nanopore Sequencing Open
Rapid and reliable detection tools are essential for disease surveillance and outbreak management, and genomic data is essential to determining pathogen origin and monitoring of transmission pathways. Low virus copy number and poor RNA qua…
View article: Iodixanol density gradients as an effective phytoplasma enrichment approach to improve genome sequencing
Iodixanol density gradients as an effective phytoplasma enrichment approach to improve genome sequencing Open
Obtaining complete phytoplasma genomes is difficult due to the lack of a culture system for these bacteria. To improve genome assembly, a non-ionic, low- and iso-osmotic iodixanol (Optiprep™) density gradient centrifugation method was deve…
View article: Implementation of GA-VirReport, a Web-Based Bioinformatics Toolkit for Post-Entry Quarantine Screening of Virus and Viroids in Plants
Implementation of GA-VirReport, a Web-Based Bioinformatics Toolkit for Post-Entry Quarantine Screening of Virus and Viroids in Plants Open
High-throughput sequencing (HTS) of host plant small RNA (sRNA) is a popular approach for plant virus and viroid detection. The major bottlenecks for implementing this approach in routine virus screening of plants in quarantine include lac…
View article: Investigating the Longevity and Infectivity of Cucumber green mottle mosaic virus in Soils of the Northern Territory, Australia
Investigating the Longevity and Infectivity of Cucumber green mottle mosaic virus in Soils of the Northern Territory, Australia Open
Cucumber green mottle mosaic virus (CGMMV) is a Tobamovirus of economic importance affecting cucurbit crops and Asian cucurbit vegetables. CGMMV was detected in the Northern Territory (NT) in September 2014, the first record for Australia,…
View article: Genome Enhanced Marker Improvement for Potato Virus Y Disease Resistance in Potato
Genome Enhanced Marker Improvement for Potato Virus Y Disease Resistance in Potato Open
Potato is an important food crop worldwide and is grown in a large number of countries. As such, the crop is under disease pressures and the need for selecting disease resistance genes during breeding programs is essential. Of particular i…
View article: Viruses in cucurbit seeds from on-line mail-order providers
Viruses in cucurbit seeds from on-line mail-order providers Open