Florence Crombé
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View article: Emergence and genetic heterogeneity of STEC O113:H4: insights from whole-genome sequences of isolates across human and non-human sources
Emergence and genetic heterogeneity of STEC O113:H4: insights from whole-genome sequences of isolates across human and non-human sources Open
The increased detection of Shiga toxin-producing Escherichia coli (STEC) O113:H4 among human cases in Belgium questions the importance of this serotype as an emerging pathogen. However, detailed information focusing on serotype O113:H4 fro…
View article: Shiga toxin-producing Escherichia coli O26:H11 associated with a cluster of haemolytic uraemic syndrome cases following the mid-July 2021 floods in Belgium
Shiga toxin-producing Escherichia coli O26:H11 associated with a cluster of haemolytic uraemic syndrome cases following the mid-July 2021 floods in Belgium Open
This report describes an HUS cluster of STEC O26:H11 (ST21, stx2a+, eae+) in Belgium following severe mid-July 2021 floods. Contaminated tap water was identified as the probable source of infection of the B…
View article: Comparison of whole genome sequencing typing tools for the typing of Belgian Legionella pneumophila outbreaks isolates
Comparison of whole genome sequencing typing tools for the typing of Belgian Legionella pneumophila outbreaks isolates Open
Whole genome sequencing (WGS) marks a turning point for outbreak investigations for microorganisms related to public health matters, like Legionella pneumophila (Lp). Here, we evaluated the available Lp WGS typing tools for isolates of pre…
View article: Intestinal Carriage of Two Distinct stx2f-Carrying Escherichia coli Strains by a Child with Uncomplicated Diarrhea
Intestinal Carriage of Two Distinct stx2f-Carrying Escherichia coli Strains by a Child with Uncomplicated Diarrhea Open
Two distinct stx2f-carrying Escherichia coli (E. coli) strains, isolated from a child with uncomplicated diarrhea fifteen weeks apart, were characterized by combining short- and long-read sequencing to compare their genetic relatedness. On…
View article: Transforming Shiga toxin-producing Escherichia coli surveillance through whole genome sequencing in food safety practices
Transforming Shiga toxin-producing Escherichia coli surveillance through whole genome sequencing in food safety practices Open
Introduction Shiga toxin-producing Escherichia coli (STEC) is a gastrointestinal pathogen causing foodborne outbreaks. Whole Genome Sequencing (WGS) in STEC surveillance holds promise in outbreak prevention and confinement, in broadening S…
View article: Molecular archaeology: individual human DNA and infection profiles from 100 year old mosquitoes
Molecular archaeology: individual human DNA and infection profiles from 100 year old mosquitoes Open
Natural history collections represent a vast biological and genetic resource, which has remained largely untapped. We revisit mosquito collections of varying ages; some made by early tropical medicine pioneers over 100 years ago. By applyi…
View article: Comparing Vaginal and Endometrial Microbiota Using Culturomics: Proof of Concept
Comparing Vaginal and Endometrial Microbiota Using Culturomics: Proof of Concept Open
It is generally accepted that microorganisms can colonize a non-pathological endometrium. However, in a clinical setting, endometrial samples are always collected by passing through the vaginal–cervical route. As such, the vaginal and cerv…
View article: Severe Neurological Involvement in an Adult with Shiga Toxin-Producing Escherichia coli-Hemolytic Uremic Syndrome Treated with Eculizumab
Severe Neurological Involvement in an Adult with Shiga Toxin-Producing Escherichia coli-Hemolytic Uremic Syndrome Treated with Eculizumab Open
A 68-year-old man with a medical history of hypertension was admitted to the emergency department for diffuse abdominal pain preceded by bloody diarrhea. Upon admission, neurological examination was normal, but he suddenly developed a left…
View article: Core Genome Sequencing Analysis of E. coli O157:H7 Unravelling Genetic Relatedness among Strains from Cattle, Beef, and Humans in Bishoftu, Ethiopia
Core Genome Sequencing Analysis of E. coli O157:H7 Unravelling Genetic Relatedness among Strains from Cattle, Beef, and Humans in Bishoftu, Ethiopia Open
E. coli O157:H7 is a known Shiga toxin-producing Escherichia coli (STEC), causing foodborne disease globally. Cattle are the main reservoir and consumption of beef and beef products contaminated with E. coli O157:H7 is an important source …
View article: Genotypic resistance determined by whole genome sequencing versus phenotypic resistance in 234 Escherichia coli isolates
Genotypic resistance determined by whole genome sequencing versus phenotypic resistance in 234 Escherichia coli isolates Open
Whole genome sequencing (WGS) enables detailed characterization of bacteria at single nucleotide resolution. It provides data about acquired resistance genes and mutations leading to resistance. Although WGS is becoming an essential tool t…
View article: Molecular characterization of extraintestinal and diarrheagenic <i>Escherichia coli</i> blood isolates
Molecular characterization of extraintestinal and diarrheagenic <i>Escherichia coli</i> blood isolates Open
Pathogenic E. coli strains can be classified into two major groups, based on the presence of specific virulence factors: extraintestinal pathogenic E. coli (ExPEC) and diarrheagenic E. coli (DEC). Several case reports …
View article: Healthcare-Associated COVID-19 across Five Pandemic Waves: Prediction Models and Genomic Analyses
Healthcare-Associated COVID-19 across Five Pandemic Waves: Prediction Models and Genomic Analyses Open
Background: Healthcare-associated SARS-CoV-2 infections need to be explored further. Our study is an analysis of hospital-acquired infections (HAIs) and ambulatory healthcare workers (aHCWs) with SARS-CoV-2 across the pandemic in a Belgian…
View article: Culturomics to Investigate the Endometrial Microbiome: Proof-of-Concept
Culturomics to Investigate the Endometrial Microbiome: Proof-of-Concept Open
The microbiome of the reproductive tract has been associated with (sub)fertility and it has been suggested that dysbiosis reduces success rates and pregnancy outcomes. The endometrial microbiome is of particular interest given the potentia…
View article: Identification of Five Serotypes of Enteropathogenic Escherichia coli from Diarrheic Calves and Healthy Cattle in Belgium and Comparative Genomics with Shigatoxigenic E. coli
Identification of Five Serotypes of Enteropathogenic Escherichia coli from Diarrheic Calves and Healthy Cattle in Belgium and Comparative Genomics with Shigatoxigenic E. coli Open
Enteropathogenic Escherichia coli (EPEC) produce attaching/effacing (AE) lesions and cause non-bloody diarrhea in mammals. A minority of bovine EPEC belong to one of the ten classical serotypes of human and bovine AE-STEC. The purpose of t…
View article: Healthcare-Associated SARS-CoV-2 Reinfection after 3 Months with a Phylogenetically Distinct Omicron Variant: A Case Report
Healthcare-Associated SARS-CoV-2 Reinfection after 3 Months with a Phylogenetically Distinct Omicron Variant: A Case Report Open
This case report describes a 60-year-old female patient suffering from systemic sclerosis, for which she received immunomodulatory drugs. Her first SARS-CoV-2-positive nasopharyngeal sample was obtained in the emergency department, on 31 J…
View article: Healthcare-associated COVID-19 across 3 pandemic waves: patient characterisation and validation of clinical definitions using genome sequencing
Healthcare-associated COVID-19 across 3 pandemic waves: patient characterisation and validation of clinical definitions using genome sequencing Open
BackgroundWorldwide, healthcare-associated SARS-CoV-2 infections are a major problem: they are associated with increased morbidity, mortality, and hospitalization costs. In-depth studies across the pandemic are crucial to understand and pr…
View article: Evaluation of Two Automated Real-Time PCR-Based Quantification Methods for Whole Blood Epstein-Barr Viral Load
Evaluation of Two Automated Real-Time PCR-Based Quantification Methods for Whole Blood Epstein-Barr Viral Load Open
View article: Molecular characterization of extraintestinal and diarrheagenic <i>Escherichia coli</i> blood isolates
Molecular characterization of extraintestinal and diarrheagenic <i>Escherichia coli</i> blood isolates Open
Pathogenic E. coli strains can be classified into two major groups, based on the presence of specific virulence factors: extraintestinal pathogenic E. coli (ExPEC) and diarrheagenic E. coli (DEC). Several case reports …
View article: Molecular characterization of extraintestinal and diarrheagenic <i>Escherichia coli</i> blood isolates
Molecular characterization of extraintestinal and diarrheagenic <i>Escherichia coli</i> blood isolates Open
Pathogenic E. coli strains can be classified into two major groups, based on the presence of specific virulence factors: extraintestinal pathogenic E. coli (ExPEC) and diarrheagenic E. coli (DEC). Several case reports …
View article: Assessment of beef carcass contamination with Salmonella and E. coli O 157 in slaughterhouses in Bishoftu, Ethiopia
Assessment of beef carcass contamination with Salmonella and E. coli O 157 in slaughterhouses in Bishoftu, Ethiopia Open
Background Salmonella and E. coli O157 are common causes of foodborne diseases. Evisceration and de-hiding steps can lead to carcass contamination during slaughter operation. In Ethiopia, information on the association between the presence…
View article: A comparison of E. coli susceptibility for amoxicillin/clavulanic acid according to EUCAST and CLSI guidelines
A comparison of E. coli susceptibility for amoxicillin/clavulanic acid according to EUCAST and CLSI guidelines Open
View article: Sink drains as reservoirs of VIM-2 metallo-β-lactamase-producing Pseudomonas aeruginosa in a Belgian intensive care unit: relation to patients investigated by whole-genome sequencing
Sink drains as reservoirs of VIM-2 metallo-β-lactamase-producing Pseudomonas aeruginosa in a Belgian intensive care unit: relation to patients investigated by whole-genome sequencing Open
View article: Validation strategy of a bioinformatics whole genome sequencing workflow for Shiga toxin-producing Escherichia coli using a reference collection extensively characterized with conventional methods
Validation strategy of a bioinformatics whole genome sequencing workflow for Shiga toxin-producing Escherichia coli using a reference collection extensively characterized with conventional methods Open
Whole genome sequencing (WGS) enables complete characterization of bacterial pathogenic isolates at single nucleotide resolution, making it the ultimate tool for routine surveillance and outbreak investigation. The lack of standardization,…
View article: Occurrence, Molecular Characteristics, and Antimicrobial Resistance of<i>Escherichia coli</i>O157 in Cattle, Beef, and Humans in Bishoftu Town, Central Ethiopia
Occurrence, Molecular Characteristics, and Antimicrobial Resistance of<i>Escherichia coli</i>O157 in Cattle, Beef, and Humans in Bishoftu Town, Central Ethiopia Open
Escherichia coli O157 is a Shiga toxin-producing E. coli causing disease in humans. Cattle are the primary reservoir of the pathogen. Information regarding the contribution of cattle to diarrheal illnesses in humans through c…
View article: The Benefits of Whole Genome Sequencing for Foodborne Outbreak Investigation from the Perspective of a National Reference Laboratory in a Smaller Country
The Benefits of Whole Genome Sequencing for Foodborne Outbreak Investigation from the Perspective of a National Reference Laboratory in a Smaller Country Open
Gradually, conventional methods for foodborne pathogen typing are replaced by whole genome sequencing (WGS). Despite studies describing the overall benefits, National Reference Laboratories of smaller countries often show slower uptake of …
View article: Genetic characterization of Shigatoxigenic and enteropathogenic <i>Escherichia coli</i> O80:H2 from diarrhoeic and septicaemic calves and relatedness to human Shigatoxigenic <i>E. coli</i> O80:H2
Genetic characterization of Shigatoxigenic and enteropathogenic <i>Escherichia coli</i> O80:H2 from diarrhoeic and septicaemic calves and relatedness to human Shigatoxigenic <i>E. coli</i> O80:H2 Open
These findings support the need to assess cattle as potential source of contamination of humans by EHEC O80:H2 and to understand the evolution of bovine and human EHEC and EPEC O80:H2.
View article: Does insecticide resistance contribute to heterogeneities in malaria transmission in The Gambia?
Does insecticide resistance contribute to heterogeneities in malaria transmission in The Gambia? Open