Florentine U. Rutaganira
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View article: Looks can be deceiving: discordances in phylogeny and morphology within loricate choanoflagellates
Looks can be deceiving: discordances in phylogeny and morphology within loricate choanoflagellates Open
Choanoflagellates are heterotrophic holozoans that are classified into two groups based on their morphology: loricates, which possess a silica-based extracellular structure, and craspedids, which do not. Although the craspedid versus loric…
View article: A stress-responsive p38 signaling axis in choanoflagellates
A stress-responsive p38 signaling axis in choanoflagellates Open
The ancestry of animal kinases that regulate cellular responses to environmental stimuli, including stress, is understudied. A phenotypic screen uncovered a heat stress-activated protein kinase (SAPK) in the closest living relatives of ani…
View article: A stress-responsive p38 signaling axis in choanoflagellates
A stress-responsive p38 signaling axis in choanoflagellates Open
Raw data for "A stress-responsive p38 signaling axis in choanoflagellates" including flow cytometry fcs files, genotyping, image segmentation output files, sequence alignments, raw counts and cell densities for figures, mass spectrometry p…
View article: Glycerol improves the viability of a cryopreserved choanoflagellate
Glycerol improves the viability of a cryopreserved choanoflagellate Open
The colonial choanoflagellate Salpingoeca rosetta is a tractable model system for studying the origins of multicellularity, but long-term storage strategies for this species have not been tested. In this study, we probed each stage of cryo…
View article: Glycerol improves the viability of a cryopreserved choanoflagellate
Glycerol improves the viability of a cryopreserved choanoflagellate Open
The colonial choanoflagellate Salpingoeca rosetta is a tractable model system for studying the origins of multicellularity, but long-term storage strategies for this species have not been tested. In this study, we probed each stage of cryo…
View article: A stress-responsive p38 signaling axis in choanoflagellates
A stress-responsive p38 signaling axis in choanoflagellates Open
Animal kinases regulate cellular responses to environmental stimuli, including cell differentiation, migration, survival, and response to stress, but the ancestry of these functions is poorly understood. Choanoflagellates, the closest livi…
View article: Total Synthesis and Functional Evaluation of IORs, Sulfonolipid‐based Inhibitors of Cell Differentiation in<i>Salpingoeca rosetta</i>
Total Synthesis and Functional Evaluation of IORs, Sulfonolipid‐based Inhibitors of Cell Differentiation in<i>Salpingoeca rosetta</i> Open
The choanoflagellate Salpingoeca rosetta is an important model system to study the evolution of multicellularity. In this study we developed a new, modular, and scalable synthesis of sulfonolipid IOR‐1A (six steps, 27 % overall yield), whi…
View article: Structural and Functional Analysis of Bacterial Sulfonosphingolipids and Rosette‐Inducing Factor 2 (RIF‐2) by Mass Spectrometry‐Guided Isolation and Total Synthesis
Structural and Functional Analysis of Bacterial Sulfonosphingolipids and Rosette‐Inducing Factor 2 (RIF‐2) by Mass Spectrometry‐Guided Isolation and Total Synthesis Open
We have analyzed the abundance of bacterial sulfonosphingolipids, including rosette‐inducing factors (RIFs), in seven bacterial prey strains by using high‐resolution tandem mass spectrometry (HRMS 2 ) and molecular networking (MN) within t…
View article: Responding and navigating racialized microaggressions in STEM
Responding and navigating racialized microaggressions in STEM Open
While it is commonly thought that microaggressions are isolated incidents, microaggressions are ingrained throughout the academic research institution (Young, Anderson and Stewart 2015; Lee et al. 2020). Persons Excluded from science becau…
View article: Spermatogonial Stem Cell Numbers Are Reduced by Transient Inhibition of GDNF Signaling but Restored by Self-Renewing Replication when Signaling Resumes
Spermatogonial Stem Cell Numbers Are Reduced by Transient Inhibition of GDNF Signaling but Restored by Self-Renewing Replication when Signaling Resumes Open
One cause of human male infertility is a scarcity of spermatogonial stem cells (SSCs) in testes with Sertoli cells that neither produce adequate amounts of GDNF nor form the Sertoli-Sertoli junctions that form the blood-testis barrier (BTB…
View article: PI4KIIIβ is a therapeutic target in chromosome 1q–amplified lung adenocarcinoma
PI4KIIIβ is a therapeutic target in chromosome 1q–amplified lung adenocarcinoma Open
This study identifies a molecular basis for malignant secretion and provides a rationale to test PI4KIIIβ antagonists as an intervention.
View article: Inhibition of Calcium Dependent Protein Kinase 1 (CDPK1) by Pyrazolopyrimidine Analogs Decreases Establishment and Reoccurrence of Central Nervous System Disease by <i>Toxoplasma gondii</i>
Inhibition of Calcium Dependent Protein Kinase 1 (CDPK1) by Pyrazolopyrimidine Analogs Decreases Establishment and Reoccurrence of Central Nervous System Disease by <i>Toxoplasma gondii</i> Open
Calcium dependent protein kinase 1 (CDPK1) is an essential enzyme in the opportunistic pathogen Toxoplasma gondii. CDPK1 controls multiple processes that are critical to the intracellular replicative cycle of T. gondii including secretion …
View article: Multistep Compositional Remodeling of Supported Lipid Membranes by Interfacially Active Phosphatidylinositol Kinases
Multistep Compositional Remodeling of Supported Lipid Membranes by Interfacially Active Phosphatidylinositol Kinases Open
The multienzyme catalytic phosphorylation of phosphatidylinositol (PI) in a supported lipid membrane platform is demonstrated for the first time. One-step treatment with PI 4-kinase IIIβ (PI4Kβ) yielded PI 4-phosphate (PI4P), while a multi…
View article: Design and Structural Characterization of Potent and Selective Inhibitors of Phosphatidylinositol 4 Kinase IIIβ
Design and Structural Characterization of Potent and Selective Inhibitors of Phosphatidylinositol 4 Kinase IIIβ Open
Type III phosphatidylinositol 4-kinase (PI4KIIIβ) is an essential enzyme in mediating membrane trafficking and is implicated in a variety of pathogenic processes. It is a key host factor mediating replication of RNA viruses. The design of …
View article: Using hydrogen deuterium exchange mass spectrometry to engineer optimized constructs for crystallization of protein complexes: Case study of PI4KIIIβ with Rab11
Using hydrogen deuterium exchange mass spectrometry to engineer optimized constructs for crystallization of protein complexes: Case study of PI4KIIIβ with Rab11 Open
The ability of proteins to bind and interact with protein partners plays fundamental roles in many cellular contexts. X‐ray crystallography has been a powerful approach to understand protein‐protein interactions; however, a challenge in th…