Florian Csarman
YOU?
Author Swipe
View article: Oxidative cleavage of methyl-esterified pectic homogalacturonan by an AA17 lytic polysaccharide monooxygenase from Phytophthora infestans
Oxidative cleavage of methyl-esterified pectic homogalacturonan by an AA17 lytic polysaccharide monooxygenase from Phytophthora infestans Open
Lytic polysaccharide monooxygenases (LPMOs) are mono‑copper-dependent enzymes that catalyze the oxidative breakdown of polysaccharides. The recently discovered AA17 family, exclusively found in oomycete genomes, plays a critical role in pl…
View article: Interaction of class <scp>III</scp> cellobiose dehydrogenase with lytic polysaccharide monooxygenase
Interaction of class <span>III</span> cellobiose dehydrogenase with lytic polysaccharide monooxygenase Open
The genome of Fusarium solani , a well‐known plant pathogen, encodes various lytic polysaccharide monooxygenases (LPMOs) involved in plant biomass degradation in combination with cellobiose dehydrogenase (CDH). To investigate the auxiliary…
View article: Electrochemical Monitoring of Heterogeneous Peroxygenase Reactions Unravels LPMO Kinetics
Electrochemical Monitoring of Heterogeneous Peroxygenase Reactions Unravels LPMO Kinetics Open
Biological conversion of plant biomass depends on peroxygenases and peroxidases acting on insoluble polysaccharides and lignin. Among these are cellulose- and hemicellulose-degrading lytic polysaccharide monooxygenases (LPMOs), which have …
View article: Amino Acid Residues Controlling Domain Interaction and Interdomain Electron Transfer in Cellobiose Dehydrogenase
Amino Acid Residues Controlling Domain Interaction and Interdomain Electron Transfer in Cellobiose Dehydrogenase Open
The function of cellobiose dehydrogenase (CDH) in biosensors, biofuel cells, and as a physiological redox partner of lytic polysaccharide monooxygenase (LPMO) is based on its role as an electron donor. Before donating electrons to LPMO or …
View article: Resolving domain positions of cellobiose dehydrogenase by small angle X‐ray scattering
Resolving domain positions of cellobiose dehydrogenase by small angle X‐ray scattering Open
The interdomain electron transfer (IET) between the catalytic flavodehydrogenase domain and the electron‐transferring cytochrome domain of cellobiose dehydrogenase (CDH) plays an essential role in biocatalysis, biosensors and biofuel cells…
View article: Towards a better understanding of synergistic enzyme effects during refining of cellulose fibers
Towards a better understanding of synergistic enzyme effects during refining of cellulose fibers Open
Refining of cellulose fibers is essential for reaching desired paper properties, yet highly energy demanding. Enzymes like endoglucanases (e.g. EndoC) are increasingly used to reduce energy consumption during pulp refining. However, predic…
View article: Fluorescent Imaging of Extracellular Fungal Enzymes Bound onto Plant Cell Walls
Fluorescent Imaging of Extracellular Fungal Enzymes Bound onto Plant Cell Walls Open
Lignocelluloytic enzymes are industrially applied as biocatalysts for the deconstruction of recalcitrant plant biomass. To study their biocatalytic and physiological function, the assessment of their binding behavior and spatial distributi…
View article: Lytic Polysaccharide Monooxygenase from Talaromyces amestolkiae with an Enigmatic Linker-like Region: The Role of This Enzyme on Cellulose Saccharification
Lytic Polysaccharide Monooxygenase from Talaromyces amestolkiae with an Enigmatic Linker-like Region: The Role of This Enzyme on Cellulose Saccharification Open
The first lytic polysaccharide monooxygenase (LPMO) detected in the genome of the widespread ascomycete Talaromyces amestolkiae (TamAA9A) has been successfully expressed in Pichia pastoris and characterized. Molecular modeling of TamAA9A s…
View article: Spectroelectrochemical investigation of the glyoxal oxidase activation mechanism
Spectroelectrochemical investigation of the glyoxal oxidase activation mechanism Open
Glyoxal oxidase (GLOX) is an extracellular source of H2O2 in white-rot secretomes, where it acts in concert with peroxidases to degrade lignin. It has been reported that GLOX requires activation prior to catalytic turnover and that a perox…
View article: Real-Time Measurement of Cellobiose and Glucose Formation during Enzymatic Biomass Hydrolysis
Real-Time Measurement of Cellobiose and Glucose Formation during Enzymatic Biomass Hydrolysis Open
Enzymatic hydrolysis of lignocellulosic biomass for biofuel production relies on complex multi-enzyme ensembles. Continuous and accurate measurement of the released key products is crucial in optimizing the industrial degradation process a…
View article: Functional expression and characterization of two laccases from the brown rot Fomitopsis pinicola
Functional expression and characterization of two laccases from the brown rot Fomitopsis pinicola Open
Laccase is predominantly found in lignin degrading filamentous white rot fungi, where it is involved in the oxidative degradation of this recalcitrant heteropolymer. In brown rot fungi it is much less prevalent: laccases from only a few br…
View article: Comparative characterization of glyoxal oxidase from Phanerochaete chrysosporium expressed at high levels in Pichia pastoris and Trichoderma reesei
Comparative characterization of glyoxal oxidase from Phanerochaete chrysosporium expressed at high levels in Pichia pastoris and Trichoderma reesei Open
In the secretome of Phanerochaete chrysosporium, a white-rot fungus serving as a model organism to elucidate lignocellulose deconstruction, the copper containing metalloprotein glyoxal oxidase (GLOX) is potentially involved in the crucial …
View article: Heterologous Expression of Phanerochaete Chrysosporium Cellobiose Dehydrogenase in Trichoderma Reesei
Heterologous Expression of Phanerochaete Chrysosporium Cellobiose Dehydrogenase in Trichoderma Reesei Open
Background: Cellobiose dehydrogenase from Phanerochaete chrysosporium ( Pc CDH) is a key enzyme in lignocellulose depolymerization, biosensors and biofuel cells. For these applications, it should retain important molecular and catalytic pr…
View article: High-resolution study of fungal enzymes
High-resolution study of fungal enzymes Open
Current processes for lignocellulose deconstruction are unspecific and produce some constituents in poor quality. Specific biocatalysts could achieve optimal segregation together with minimal damage to cellulose and lignin and provide high…
View article: Influence of Lytic Polysaccharide Monooxygenase Active Site Segments on Activity and Affinity
Influence of Lytic Polysaccharide Monooxygenase Active Site Segments on Activity and Affinity Open
In past years, new lytic polysaccharide monooxygenases (LPMOs) have been discovered as distinct in their substrate specificity. Their unconventional, surface-exposed catalytic sites determine their enzymatic activities, while binding sites…
View article: Evolving stability and pH-dependent activity of the high redox potential Botrytis aclada laccase for enzymatic fuel cells
Evolving stability and pH-dependent activity of the high redox potential Botrytis aclada laccase for enzymatic fuel cells Open
Fungal high redox potential laccases are proposed as cathodic biocatalysts in implantable enzymatic fuel cells to generate high cell voltages. Their application is limited mainly through their acidic pH optimum and chloride inhibition. Thi…