Florian Praetorius
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View article: Design of facilitated dissociation enables timing of cytokine signalling
Design of facilitated dissociation enables timing of cytokine signalling Open
View article: Design of facilitated dissociation enables control over cytokine signaling duration
Design of facilitated dissociation enables control over cytokine signaling duration Open
Protein design has focused primarily on the design of ground states, ensuring they are sufficiently low energy to be highly populated. Designing the kinetics and dynamics of a system requires, in addition, the design of excited states that…
View article: De novo design of allosterically switchable protein assemblies
De novo design of allosterically switchable protein assemblies Open
Allosteric modulation of protein function, wherein the binding of an effector to a protein triggers conformational changes at distant functional sites, plays a central part in the control of metabolism and cell signalling1-3. Th…
View article: Modulation of FGF pathway signaling and vascular differentiation using designed oligomeric assemblies
Modulation of FGF pathway signaling and vascular differentiation using designed oligomeric assemblies Open
Many growth factors and cytokines signal by binding to the extracellular domains of their receptors and driving association and transphosphorylation of the receptor intracellular tyrosine kinase domains, initiating downstream signaling cas…
View article: De novo design of allosterically switchable protein assemblies
De novo design of allosterically switchable protein assemblies Open
Allosteric modulation of protein function, wherein the binding of an effector to a protein triggers conformational changes at distant functional sites, plays a central role in the control of metabolism and cell signaling 1–3 . There has be…
View article: Design of stimulus-responsive two-state hinge proteins
Design of stimulus-responsive two-state hinge proteins Open
In nature, proteins that switch between two conformations in response to environmental stimuli structurally transduce biochemical information in a manner analogous to how transistors control information flow in computing devices. Designing…
View article: Data for: Design of stimulus-responsive two-state hinge proteins
Data for: Design of stimulus-responsive two-state hinge proteins Open
Supplementary information for Design of stimulus-responsive two-state hinge proteins: Design scripts, design models, and aw DEER data.
View article: Data for: Design of stimulus-responsive two-state hinge proteins
Data for: Design of stimulus-responsive two-state hinge proteins Open
Supplementary information for Design of stimulus-responsive two-state hinge proteins: Design scripts, design models, and aw DEER data.
View article: Modulation of FGF pathway signaling and vascular differentiation using designed oligomeric assemblies
Modulation of FGF pathway signaling and vascular differentiation using designed oligomeric assemblies Open
Growth factors and cytokines signal by binding to the extracellular domains of their receptors and drive association and transphosphorylation of the receptor intracellular tyrosine kinase domains, initiating downstream signaling cascades. …
View article: Design of stimulus-responsive two-state hinge proteins
Design of stimulus-responsive two-state hinge proteins Open
Proteins that switch between two structural states as a function of environmental stimuli are widespread in nature. These proteins structurally transduce biochemical information in a manner analogous to how transistors control information …
View article: Reconfigurable asymmetric protein assemblies through implicit negative design
Reconfigurable asymmetric protein assemblies through implicit negative design Open
Asymmetric multiprotein complexes that undergo subunit exchange play central roles in biology but present a challenge for design because the components must not only contain interfaces that enable reversible association but also be stable …
View article: Data for: Reconfigurable asymmetric protein assemblies through implicit negative design
Data for: Reconfigurable asymmetric protein assemblies through implicit negative design Open
Supplementary Information for Reconfigurable asymmetric protein assemblies through implicit negative design: design scripts, design models and sequence information Authors: Danny D. Sahtoe*, Florian Praetorius*,
View article: Data for: Reconfigurable asymmetric protein assemblies through implicit negative design
Data for: Reconfigurable asymmetric protein assemblies through implicit negative design Open
Supplementary Information for Reconfigurable asymmetric protein assemblies through implicit negative design: design scripts, design models and sequence information Authors: Danny D. Sahtoe*, Florian Praetorius*,
View article: Reconfigurable asymmetric protein assemblies through implicit negative design
Reconfigurable asymmetric protein assemblies through implicit negative design Open
Asymmetric multi-protein complexes that undergo subunit exchange play central roles in biology, but present a challenge for protein design. The individual components must contain interfaces enabling reversible addition to and dissociation …
View article: Custom-Size, Functional, and Durable DNA Origami with Design-Specific Scaffolds
Custom-Size, Functional, and Durable DNA Origami with Design-Specific Scaffolds Open
DNA origami nano-objects are usually designed around generic single-stranded "scaffolds". Many properties of the target object are determined by details of those generic scaffold sequences. Here, we enable designers to fully specify the ta…
View article: Structure and mechanism of the two-component α-helical pore-forming toxin YaxAB
Structure and mechanism of the two-component α-helical pore-forming toxin YaxAB Open
View article: Genetically encoding the spatial arrangement of DNA and proteins in self-assembling nanostructures
Genetically encoding the spatial arrangement of DNA and proteins in self-assembling nanostructures Open
Function and activity of biomolecules often depend on their spatial arrangement. The method introduced here allows genetically encoding the spatial arrangement of proteins and DNA. The approach relies on staple proteins that fold double-st…
View article: Biotechnological mass production of DNA origami
Biotechnological mass production of DNA origami Open
View article: Molecular engineering of chiral colloidal liquid crystals using DNA origami
Molecular engineering of chiral colloidal liquid crystals using DNA origami Open
View article: Genetically Encoded DNA-Protein Hybrid Origami
Genetically Encoded DNA-Protein Hybrid Origami Open
View article: Design of a molecular support for cryo-EM structure determination
Design of a molecular support for cryo-EM structure determination Open
Significance As the scope of macromolecular structure determination by cryo-electron microscopy (cryo-EM) is expanding rapidly, it is becoming increasingly clear that many biological complexes are too fragile to withstand the harsh conditi…
View article: Efficient Production of Single-Stranded Phage DNA as Scaffolds for DNA Origami
Efficient Production of Single-Stranded Phage DNA as Scaffolds for DNA Origami Open
Scaffolded DNA origami enables the fabrication of a variety of complex nanostructures that promise utility in diverse fields of application, ranging from biosensing over advanced therapeutics to metamaterials. The broad applicability of DN…