Francesco Iannelli
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View article: A 69.9-kb long inverted repeat increases genome instability in a strain of <i>Lactobacillus crispatus</i>
A 69.9-kb long inverted repeat increases genome instability in a strain of <i>Lactobacillus crispatus</i> Open
Long inverted repeats (LIRs) of DNA sequences longer than 30 kb are rare in prokaryotes. Here, we identified two 69.9-kb LIRs in the genome of Lactobacillus crispatus M247_Siena, a derivative of strain M247. Complete genome sequence of M24…
View article: Post-pandemic upsurge in Group A <i>Streptococcus</i> infections at an Italian tertiary university hospital
Post-pandemic upsurge in Group A <i>Streptococcus</i> infections at an Italian tertiary university hospital Open
Streptococcus pyogenes (Group A Streptococcus ; GAS) is a pathogen of global significance. In the pre-antibiotic era, GAS was a major cause of childhood morbidity and mortality, but its spread rapidly declined until the mid-2010s. The cont…
View article: Generation and Characterization of Stable Small Colony Variants of USA300 Staphylococcus aureus in RAW 264.7 Murine Macrophages
Generation and Characterization of Stable Small Colony Variants of USA300 Staphylococcus aureus in RAW 264.7 Murine Macrophages Open
Intracellular survival and immune evasion are typical features of staphylococcal infections. USA300 is a major clone of methicillin-resistant S. aureus (MRSA), a community- and hospital-acquired pathogen capable of disseminating throughout…
View article: Differential gene expression analysis pipelines and bioinformatic tools for the identification of specific biomarkers: A review
Differential gene expression analysis pipelines and bioinformatic tools for the identification of specific biomarkers: A review Open
View article: The mobilome of Lactobacillus crispatus M247 includes two novel genetic elements: Tn7088 coding for a putative bacteriocin and the siphovirus prophage ΦM247
The mobilome of Lactobacillus crispatus M247 includes two novel genetic elements: Tn7088 coding for a putative bacteriocin and the siphovirus prophage ΦM247 Open
Lactobacillus crispatus is a member of the vaginal and gastrointestinal human microbiota. Here we determined the complete genome sequence of the probiotic strain M247 combining Nanopore and Illumina technologies. The M247 genome is organiz…
View article: The <i>mef</i> (A)/ <i>msr</i> (D)-carrying streptococcal prophage Φ1207.3 encodes an SOS-like system, induced by UV-C light, responsible for increased survival and increased mutation rate
The <i>mef</i> (A)/ <i>msr</i> (D)-carrying streptococcal prophage Φ1207.3 encodes an SOS-like system, induced by UV-C light, responsible for increased survival and increased mutation rate Open
Bacterial SOS response is an inducible system of DNA repair and mutagenesis. Streptococci lack a canonical SOS response, but an SOS-like response was reported in some species. The mef (A)- msr (D)-carrying prophage Ф1207.3 of Streptococcus…
View article: The Mobilome-Enriched Genome of the Competence-Deficient Streptococcus pneumoniae BM6001, the Original Host of Integrative Conjugative Element Tn5253, Is Phylogenetically Distinct from Historical Pneumococcal Genomes
The Mobilome-Enriched Genome of the Competence-Deficient Streptococcus pneumoniae BM6001, the Original Host of Integrative Conjugative Element Tn5253, Is Phylogenetically Distinct from Historical Pneumococcal Genomes Open
Streptococcus pneumoniae is an important human pathogen causing both mild and severe diseases. In this work, we determined the complete genome sequence of the S. pneumoniae clinical isolate BM6001, which is the original host of the ICE Tn5…
View article: Streptococcus pyogenes Φ1207.3 Is a Temperate Bacteriophage Carrying the Macrolide Resistance Gene Pair <i>mef</i> (A)- <i>msr</i> (D) and Capable of Lysogenizing Different Streptococci
Streptococcus pyogenes Φ1207.3 Is a Temperate Bacteriophage Carrying the Macrolide Resistance Gene Pair <i>mef</i> (A)- <i>msr</i> (D) and Capable of Lysogenizing Different Streptococci Open
Bacteriophages play an important role in bacterial physiology and genome evolution. The widespread use of genome sequencing revealed that bacterial genomes can contain several different integrated temperate bacteriophages, which can consti…
View article: Analysis of Primary Cilium Expression and Hedgehog Pathway Activation in Mesothelioma Throws Back Its Complex Biology
Analysis of Primary Cilium Expression and Hedgehog Pathway Activation in Mesothelioma Throws Back Its Complex Biology Open
The primary cilium (PC) is a sensory organelle present on the cell surface, modulating the activity of many pathways. Dysfunctions in the PC lead to different pathologic conditions including cancer. Hedgehog signaling (Hh) is regulated by …
View article: <i>Streptococcus pyogenes</i>ϕ1207.3 is a temperate bacteriophage carrying the macrolide efflux gene pair<i>mef</i>(A)-<i>msr</i>(D) and capable to lysogenise different Streptococci
<i>Streptococcus pyogenes</i>ϕ1207.3 is a temperate bacteriophage carrying the macrolide efflux gene pair<i>mef</i>(A)-<i>msr</i>(D) and capable to lysogenise different Streptococci Open
Streptococcus pyogenes prophage ϕ1207.3 (formerly Tn 1207.3 ) carries the mef (A)- msr (D) efflux resistance genes, responsible for type M macrolide resistance. To investigate if ϕ1207.3 is a functional bacteriophage, we transferred the el…
View article: Comprehensive Study of Antiretroviral Drug Permeability at the Cervicovaginal Mucosa via an In Vitro Model
Comprehensive Study of Antiretroviral Drug Permeability at the Cervicovaginal Mucosa via an In Vitro Model Open
Modulation of drug transporter activity at mucosal sites of HIV-1 transmission may be exploited to optimize retention of therapeutic antiretroviral drug concentrations at target submucosal CD4+ T cells. Previously, we showed that darunavir…
View article: Immune Memory After Respiratory Infection With Streptococcus pneumoniae Is Revealed by in vitro Stimulation of Murine Splenocytes With Inactivated Pneumococcal Whole Cells: Evidence of Early Recall Responses by Transcriptomic Analysis
Immune Memory After Respiratory Infection With Streptococcus pneumoniae Is Revealed by in vitro Stimulation of Murine Splenocytes With Inactivated Pneumococcal Whole Cells: Evidence of Early Recall Responses by Transcriptomic Analysis Open
The in vitro stimulation of immune system cells with live or killed bacteria is essential for understanding the host response to pathogens. In the present study, we propose a model combining transcriptomic and cytokine assays on murine spl…
View article: Genome sequence typing and antimicrobial susceptibility testing of infertility-associated Enterococcus faecalis reveals clonality of aminoglycoside-resistant strains
Genome sequence typing and antimicrobial susceptibility testing of infertility-associated Enterococcus faecalis reveals clonality of aminoglycoside-resistant strains Open
View article: DNA isolation methods for Nanopore sequencing of the Streptococcus mitis genome
DNA isolation methods for Nanopore sequencing of the Streptococcus mitis genome Open
Streptococcus mitis is a Gram-positive bacterium, member of the oral commensal microbiota, which can occasionally be the etiologic agent of diseases such as infective endocarditis, bacteraemia and septicaemia. The highly recombinogenic and…
View article: Complete Genome Sequences of Mycobacterium chimaera Strains 850 and 852, Isolated from Heater-Cooler Unit Water
Complete Genome Sequences of Mycobacterium chimaera Strains 850 and 852, Isolated from Heater-Cooler Unit Water Open
The whole-genome sequences of Mycobacterium chimera strains 850 and 852, which were isolated from two different water samples obtained from a heater-cooler unit at Siena Hospital (Italy), were determined by combining Nanopore and Illumina …
View article: Predicted transmembrane proteins with homology to Mef(A) are not responsible for complementing mef(A) deletion in the mef(A)–msr(D) macrolide efflux system in Streptococcus pneumoniae
Predicted transmembrane proteins with homology to Mef(A) are not responsible for complementing mef(A) deletion in the mef(A)–msr(D) macrolide efflux system in Streptococcus pneumoniae Open
View article: Chromosomal integration of Tn5253 occurs downstream of a conserved 11-bp sequence of the rbgA gene in Streptococcus pneumoniae and in all the other known hosts of this integrative conjugative element (ICE)
Chromosomal integration of Tn5253 occurs downstream of a conserved 11-bp sequence of the rbgA gene in Streptococcus pneumoniae and in all the other known hosts of this integrative conjugative element (ICE) Open
View article: Complete Genome Sequence of Streptococcus pneumoniae Strain Rx1, a Hex Mismatch Repair-Deficient Standard Transformation Recipient
Complete Genome Sequence of Streptococcus pneumoniae Strain Rx1, a Hex Mismatch Repair-Deficient Standard Transformation Recipient Open
The complete genome sequence of Streptococcus pneumoniae strain Rx1, a Hex mismatch repair-deficient standard transformation recipient, was obtained by combining Nanopore and Illumina sequencing technologies. The genome consists of a 2.03-…
View article: A Mating Procedure for Genetic Transfer of Integrative and Conjugative Elements (ICEs) of Streptococci and Enterococci
A Mating Procedure for Genetic Transfer of Integrative and Conjugative Elements (ICEs) of Streptococci and Enterococci Open
DNA sequencing of whole bacterial genomes has revealed that the entire set of mobile genes (mobilome) represents as much as 25% of the bacterial genome. Despite the huge availability of sequence data, the functional analysis of the mobile …
View article: Complete Genome Sequence of Lactobacillus crispatus Type Strain ATCC 33820
Complete Genome Sequence of Lactobacillus crispatus Type Strain ATCC 33820 Open
The complete genome sequence of Lactobacillus crispatus type strain ATCC 33820 was obtained by combining Nanopore and Illumina sequencing technologies. The genome consists of a 2.2-Mb circular chromosome with 2,194 open reading frames and …
View article: Additional file 1 of Chromosomal integration of Tn5253 occurs downstream of a conserved 11-bp sequence of the rbgA gene in Streptococcus pneumoniae and in all the other known hosts of this integrative conjugative element (ICE)
Additional file 1 of Chromosomal integration of Tn5253 occurs downstream of a conserved 11-bp sequence of the rbgA gene in Streptococcus pneumoniae and in all the other known hosts of this integrative conjugative element (ICE) Open
Additional file 1: Table S1. Blast searches output of the S. pneumoniae complete genomes. For each variant, name, length, sequence, strain host, GenBank accession number, are reported.
View article: Additional file 2 of Chromosomal integration of Tn5253 occurs downstream of a conserved 11-bp sequence of the rbgA gene in Streptococcus pneumoniae and in all the other known hosts of this integrative conjugative element (ICE)
Additional file 2 of Chromosomal integration of Tn5253 occurs downstream of a conserved 11-bp sequence of the rbgA gene in Streptococcus pneumoniae and in all the other known hosts of this integrative conjugative element (ICE) Open
Additional file 2: Table S2. Blast searches output of the complete microbial genomes. For each attB site, name, length, sequence, bacterial species host, GenBank accession number, are reported.
View article: Additional file 4 of Chromosomal integration of Tn5253 occurs downstream of a conserved 11-bp sequence of the rbgA gene in Streptococcus pneumoniae and in all the other known hosts of this integrative conjugative element (ICE)
Additional file 4 of Chromosomal integration of Tn5253 occurs downstream of a conserved 11-bp sequence of the rbgA gene in Streptococcus pneumoniae and in all the other known hosts of this integrative conjugative element (ICE) Open
Additional file 4: Table S4. Quantitative PCR data. The threshold cycles (Cts) relative to circular forms (CI), reconstitution of attB site, and chromosomal reference gene (gyrB) quantification are reported. Standard curves, slope and inte…
View article: Genomics and Genetics of <i>Streptococcus pneumoniae</i>
Genomics and Genetics of <i>Streptococcus pneumoniae</i> Open
Ninety years after the discovery of pneumococcal Transformation, and 74 years after the work of Avery and colleagues that identified DNA as the genetic material, Streptococcus pneumoniae is still one of the most important model organism to…
View article: Excision and Circularization of Integrative Conjugative Element Tn5253 of Streptococcus pneumoniae
Excision and Circularization of Integrative Conjugative Element Tn5253 of Streptococcus pneumoniae Open
The integrative conjugative element (ICE) Tn5253 of Streptococcus pneumoniae, conferring resistance to tetracycline and chloramphenicol, was found integrated at a 83-bp specific target site (attB) located in the rbg…
View article: Type M Resistance to Macrolides Is Due to a Two-Gene Efflux Transport System of the ATP-Binding Cassette (ABC) Superfamily
Type M Resistance to Macrolides Is Due to a Two-Gene Efflux Transport System of the ATP-Binding Cassette (ABC) Superfamily Open
The mef(A) gene was originally identified as the resistance determinant responsible for type M resistance to macrolides, a phenotype frequently found in clinical isolates of Streptococcus pneumoniae and Streptococcus pyoge…
View article: Mycobacterium sherrisii visceral disseminated infection in an African HIV-infected adolescent
Mycobacterium sherrisii visceral disseminated infection in an African HIV-infected adolescent Open
A case of visceral disseminated infection by Mycobacterium sherrisii in an African HIV-infected adolescent with multiple abdominal abscesses is reported. Despite multiple drug resistance to first-line antibiotics in vitro, long-term treatm…
View article: Interferon-γ from Brain Leukocytes Enhances Meningitis by Type 4 Streptococcus pneumoniae
Interferon-γ from Brain Leukocytes Enhances Meningitis by Type 4 Streptococcus pneumoniae Open
Streptococcus pneumoniae is the leading cause of bacterial meningitis. Pneumococcal meningitis is a life-threatening disease with high rates of mortality and neurological sequelae. Immune targeting of S. pneumoniae is essential for clearan…
View article: Drug transporter gene expression in human colorectal tissue and cell lines: modulation with antiretrovirals for microbicide optimization
Drug transporter gene expression in human colorectal tissue and cell lines: modulation with antiretrovirals for microbicide optimization Open
These findings will help optimize complex formulations of rectal microbicides to realize their full potential as an effective approach for pre-exposure prophylaxis against HIV-1 infection.
View article: Expression of Genes for Drug Transporters in the Human Female Genital Tract and Modulatory Effect of Antiretroviral Drugs
Expression of Genes for Drug Transporters in the Human Female Genital Tract and Modulatory Effect of Antiretroviral Drugs Open
Anti-retroviral (ARV) -based microbicides are one of the strategies pursued to prevent HIV-1 transmission. Delivery of ARV drugs to subepithelial CD4+ T cells at concentrations for protection is likely determined by drug transporters expre…