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View article: Rare Ketose Synthesis from Glycolaldehyde by C2 Elongation of Aldoses Catalyzed by Transketolase Variants
Rare Ketose Synthesis from Glycolaldehyde by C2 Elongation of Aldoses Catalyzed by Transketolase Variants Open
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View article: Extending the toolbox for enzymatic carboligation: synthesis of α-hydroxyketones catalyzed by thermostable transketolase from Geobacillus stearothermophilus
Extending the toolbox for enzymatic carboligation: synthesis of α-hydroxyketones catalyzed by thermostable transketolase from Geobacillus stearothermophilus Open
Enzymatic carbon–carbon (C–C) bond formation represents an efficient asymmetric alternative for the preparation of multifunctional products. This paper presents an overview of the advances made by engineering a thermostable thiamine-depend…
View article: Enzymatic Upgrading of Biomass‐Derived Aldoses to Rare Deoxy Ketoses Catalyzed by Transketolase Variants
Enzymatic Upgrading of Biomass‐Derived Aldoses to Rare Deoxy Ketoses Catalyzed by Transketolase Variants Open
A sustainable, convenient, scalable, one‐step method for the two‐carbon chain elongation of cheap and biomass‐derived pentoses ( l ‐arabinose, and 2‐deoxy‐ d ‐ribose) and hexose l ‐rhamnose was developed to produce C n+2 deoxy ketoses (C‐7…
View article: Cross-acyloin condensation of aldehydes catalysed by transketolase variants for the synthesis of aliphatic α-hydroxyketones
Cross-acyloin condensation of aldehydes catalysed by transketolase variants for the synthesis of aliphatic α-hydroxyketones Open
Transketolase variants catalyse a promiscuous acyloin condensation of aliphatic aldehydes leading in one step to unsymmetrical α-hydroxyketones while offering green advances compared to previous enzymatic and chemical ways.
View article: Structural determination and kinetic analysis of the transketolase from <i>Vibrio vulnificus</i> reveal unexpected cooperative behavior
Structural determination and kinetic analysis of the transketolase from <i>Vibrio vulnificus</i> reveal unexpected cooperative behavior Open
Vibrio vulnificus ( vv ) is a multidrug‐resistant human bacterial pathogen whose prevalence is expected to increase over the years. Transketolases (TK), transferases catalyzing two reactions of the nonoxidative branch of the pentose‐phosph…
View article: Co-Immobilization of D-Amino Acid Oxidase, Catalase, and Transketolase for One-Pot, Two-Step Synthesis of L-Erythrulose
Co-Immobilization of D-Amino Acid Oxidase, Catalase, and Transketolase for One-Pot, Two-Step Synthesis of L-Erythrulose Open
Here, we present an immobilized enzyme cascade in a basket-type reactor allowing a one-pot, two-step enzymatic synthesis of L-erythrulose from D-serine and glycolaldehyde. Three enzymes, D-amino acid oxidase from Rhodotorula gracilis (DAAO…
View article: Synthesis of Deoxysugars from Aliphatic α‐Ketoacids and Aldoses Catalyzed by Thermostable Transketolase Variants from <i>Geobacillus stearothermophilus</i>
Synthesis of Deoxysugars from Aliphatic α‐Ketoacids and Aldoses Catalyzed by Thermostable Transketolase Variants from <i>Geobacillus stearothermophilus</i> Open
We described a strategy for the enzymatic synthesis of 1‐deoxy and 1,2‐deoxyketoses from the aliphatic α‐ketoacids, pyruvate and 2‐oxobutyrate, as donors and natural aldoses of variable chain length as acceptors, catalyzed by thermostable …
View article: Cleavage of Aliphatic α-Hydroxy Ketones by Evolved Transketolase from <i>Geobacillus stearothermophilus</i>
Cleavage of Aliphatic α-Hydroxy Ketones by Evolved Transketolase from <i>Geobacillus stearothermophilus</i> Open
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View article: High‐Throughput Solid‐Phase Assay for Substrate Profiling and Directed Evolution of Transketolase
High‐Throughput Solid‐Phase Assay for Substrate Profiling and Directed Evolution of Transketolase Open
Thiamine diphosphate‐dependent enzymes, and specifically transketolases, form one of the most important families of biocatalytic tools for enantioselective carbon‐carbon bond formation yielding various hydroxyketones of biological interest…
View article: Biocatalysed synthesis of chiral amines: continuous colorimetric assays for mining amine-transaminases
Biocatalysed synthesis of chiral amines: continuous colorimetric assays for mining amine-transaminases Open
Versatile and sensitive continuous colorimetric assays were developed for the high throughput screening of a large collection of amine-TAs from biodiversity, and allowed the discovery of a set of diverse biocatalysts with high synthetic po…
View article: Enzymatic Synthesis of Aliphatic Acyloins Catalyzed by Thermostable Transketolase
Enzymatic Synthesis of Aliphatic Acyloins Catalyzed by Thermostable Transketolase Open
The thermostable transketolase from Geobacillus stearothermophilus (TK gst ) was successfully engineered for the synthesis of aliphatic acyloins with varying carbon backbone lengths (C 5 −C 10 ) based on protein structure‐guided studies. E…
View article: <scp>d</scp>-Serine as a Key Building Block: Enzymatic Process Development and Smart Applications within the Cascade Enzymatic Concept
<span>d</span>-Serine as a Key Building Block: Enzymatic Process Development and Smart Applications within the Cascade Enzymatic Concept Open
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View article: Convergent <i>in situ</i> Generation of Both Transketolase Substrates <i>via</i> Transaminase and Aldolase Reactions for Sequential One‐Pot, Three‐Step Cascade Synthesis of Ketoses
Convergent <i>in situ</i> Generation of Both Transketolase Substrates <i>via</i> Transaminase and Aldolase Reactions for Sequential One‐Pot, Three‐Step Cascade Synthesis of Ketoses Open
We describe an efficient three‐enzyme, sequential one‐pot cascade reaction where both transketolase substrates are generated in situ in a convergent fashion. The nucleophilic donor substrate hydroxypyruvate was obtained from l ‐serine and …
View article: One‐Pot Cascade Synthesis of (3<i>S</i>)‐Hydroxyketones Catalyzed by Transketolase <i>via</i> Hydroxypyruvate Generated <i>in Situ</i> from <scp>d</scp>‐Serine by <scp>d</scp>‐Amino Acid Oxidase
One‐Pot Cascade Synthesis of (3<i>S</i>)‐Hydroxyketones Catalyzed by Transketolase <i>via</i> Hydroxypyruvate Generated <i>in Situ</i> from <span>d</span>‐Serine by <span>d</span>‐Amino Acid Oxidase Open
We described an efficient in situ generation of hydroxypyruvate from d ‐serine catalyzed by a d ‐amino acid oxidase from Rhodotorula gracilis . This strategy revealed an interesting alternative to the conventional chemical synthesis of hyd…
View article: Enantioselective Synthesis of <scp>d</scp>‐ and <scp>l</scp>‐α‐Amino Acids by Enzymatic Transamination Using Glutamine as Smart Amine Donor
Enantioselective Synthesis of <span>d</span>‐ and <span>l</span>‐α‐Amino Acids by Enzymatic Transamination Using Glutamine as Smart Amine Donor Open
Enzymatic transamination is a useful method for the green and highly enantioselective synthesis of chiral amines and non‐canonical amino acids which are of major importance as intermediates in medicinal chemistry. However, transamination r…
View article: Dosages colorimétriques continus à haut débit pour les alpha-transaminases.
Dosages colorimétriques continus à haut débit pour les alpha-transaminases. Open
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View article: Stereoselective synthesis of γ-hydroxy-α-amino acids through aldolase–transaminase recycling cascades
Stereoselective synthesis of γ-hydroxy-α-amino acids through aldolase–transaminase recycling cascades Open
Efficient aldolase (AL)–transaminase (TA) recycling cascades were designed for the stereoselective synthesis of γ-hydroxy-α-amino acids with high atom economy.
View article: Droplet millifluidics for kinetic study of transketolase
Droplet millifluidics for kinetic study of transketolase Open
We present a continuous-flow reactor at the millifluidic scale coupled with an online, non-intrusive spectroscopic monitoring method for determining the kinetic parameters of an enzyme, transketolase (TK) used in biocatalysis for the synth…
View article: Engineering a thermostable transketolase for arylated substrates
Engineering a thermostable transketolase for arylated substrates Open
Transketolase variants were engineered to utilize arylalkanals and benzaldehyde as substrates with up to 28-fold rate acceleration for C–C bond formation with good yields (50–73%) and virtually complete (3S)-stereoselectivity (>99% ee).
View article: One-pot, two-step cascade synthesis of naturally rare<scp>l</scp>-erythro (3S,4S) ketoses by coupling a thermostable transaminase and transketolase
One-pot, two-step cascade synthesis of naturally rare<span>l</span>-erythro (3S,4S) ketoses by coupling a thermostable transaminase and transketolase Open
Naturally rarel-erythro(3S,4S)-ketoses were prepared at high temperatures through a simultaneous two-step enzymatic cascade synthesis with excellent stereoselectivity.
View article: Galactose Oxidase/Prussian Blue Based Biosensors
Galactose Oxidase/Prussian Blue Based Biosensors Open
This paper describes the development of an amperometric biosensor based on galactose oxidase (GAOx) immobilization within a laponite clay film deposited on Carbon Screen‐Printed Electrodes modified by electrodeposited Prussian Blue and coa…