François Kroll
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View article: Neural microexons modulate arousal states via cAMP signalling in zebrafish
Neural microexons modulate arousal states via cAMP signalling in zebrafish Open
Arousal states, often dysregulated in neurodevelopmental disorders, shape how organisms perceive and respond to their environment. Here, we show that srrm3 , a master regulator of neural microexons, is essential for normal arousal in zebra…
View article: JACUZI-SD: An automated, high-throughput, minimally stressful approach to sleep depriving larval zebrafish
JACUZI-SD: An automated, high-throughput, minimally stressful approach to sleep depriving larval zebrafish Open
While sleep deprivation broadly disrupts health and well-being, the neural and molecular mechanisms that signal increased sleep pressure remain poorly understood. A key obstacle to progress is the fact that traditional methods for inducing…
View article: Author response: Behavioural pharmacology predicts disrupted signalling pathways and candidate therapeutics from zebrafish mutants of Alzheimer’s disease risk genes
Author response: Behavioural pharmacology predicts disrupted signalling pathways and candidate therapeutics from zebrafish mutants of Alzheimer’s disease risk genes Open
View article: Behavioural pharmacology predicts disrupted signalling pathways and candidate therapeutics from zebrafish mutants of Alzheimer’s disease risk genes
Behavioural pharmacology predicts disrupted signalling pathways and candidate therapeutics from zebrafish mutants of Alzheimer’s disease risk genes Open
By exposing genes associated with disease, genomic studies provide hundreds of starting points that should lead to druggable processes. However, our ability to systematically translate these genomic findings into biological pathways remain…
View article: Behavioural pharmacology predicts disrupted signalling pathways and candidate therapeutics from zebrafish mutants of Alzheimer’s disease risk genes
Behavioural pharmacology predicts disrupted signalling pathways and candidate therapeutics from zebrafish mutants of Alzheimer’s disease risk genes Open
By exposing genes associated with disease, genomic studies provide hundreds of starting points that should lead to druggable processes. However, our ability to systematically translate these genomic findings into biological pathways remain…
View article: Author response: Behavioural pharmacology predicts disrupted signalling pathways and candidate therapeutics from zebrafish mutants of Alzheimer’s disease risk genes
Author response: Behavioural pharmacology predicts disrupted signalling pathways and candidate therapeutics from zebrafish mutants of Alzheimer’s disease risk genes Open
View article: Dual-targeting CRISPR-CasRx reduces C9orf72 ALS/FTD sense and antisense repeat RNAs in vitro and in vivo
Dual-targeting CRISPR-CasRx reduces C9orf72 ALS/FTD sense and antisense repeat RNAs in vitro and in vivo Open
The most common genetic cause of frontotemporal dementia (FTD) and amyotrophic lateral sclerosis (ALS) is an intronic G 4 C 2 repeat expansion in C9orf72 . The repeats undergo bidirectional transcription to produce sense and antisense repe…
View article: Author response: Behavioural pharmacology predicts disrupted signalling pathways and candidate therapeutics from zebrafish mutants of Alzheimer’s disease risk genes
Author response: Behavioural pharmacology predicts disrupted signalling pathways and candidate therapeutics from zebrafish mutants of Alzheimer’s disease risk genes Open
By exposing genes associated with disease, genomic studies provide hundreds of starting points that should lead to druggable processes. However, our ability to systematically translate these genomic findings into biological pathways remain…
View article: Behavioural pharmacology predicts disrupted signalling pathways and candidate therapeutics from zebrafish mutants of Alzheimer’s disease risk genes
Behavioural pharmacology predicts disrupted signalling pathways and candidate therapeutics from zebrafish mutants of Alzheimer’s disease risk genes Open
By exposing genes associated with disease, genomic studies provide hundreds of starting points that should lead to druggable processes. However, our ability to systematically translate these genomic findings into biological pathways remain…
View article: Behavioural pharmacology predicts disrupted signalling pathways and candidate therapeutics from zebrafish mutants of Alzheimer’s disease risk genes
Behavioural pharmacology predicts disrupted signalling pathways and candidate therapeutics from zebrafish mutants of Alzheimer’s disease risk genes Open
By exposing genes associated with disease, genomic studies provide hundreds of starting points that should lead to druggable processes. However, our ability to systematically translate these genomic findings into biological pathways remain…
View article: KASP genotyping v2
KASP genotyping v2 Open
KASP is a genotyping assay which you can use to differentiate wild types vs heterozygous vs homozygous larvae/finclips. It is well suited to genotype a SNP or a small CRISPR-generated indel. Once you confirmed it is working well, the assay…
View article: Dual-targeting CRISPR-CasRx reduces<i>C9orf72</i>ALS/FTD sense and antisense repeat RNAs in vitro and in vivo
Dual-targeting CRISPR-CasRx reduces<i>C9orf72</i>ALS/FTD sense and antisense repeat RNAs in vitro and in vivo Open
The most common genetic cause of both frontotemporal dementia (FTD) and amyotrophic lateral sclerosis (ALS) is a G 4 C 2 repeat expansion in intron 1 of the C9orf72 gene. This repeat expansion undergoes bidirectional transcription to produ…
View article: Behavioural pharmacology predicts disrupted signalling pathways and candidate therapeutics from zebrafish mutants of Alzheimer’s disease risk genes
Behavioural pharmacology predicts disrupted signalling pathways and candidate therapeutics from zebrafish mutants of Alzheimer’s disease risk genes Open
By exposing genes associated with disease, genomic studies provide hundreds of starting points that should lead to druggable processes. However, our ability to systematically translate these genomic findings into biological pathways remain…
View article: linc-mipep and linc-wrb encode micropeptides that regulate chromatin accessibility in vertebrate-specific neural cells
linc-mipep and linc-wrb encode micropeptides that regulate chromatin accessibility in vertebrate-specific neural cells Open
Thousands of long intergenic non-coding RNAs (lincRNAs) are transcribed throughout the vertebrate genome. A subset of lincRNAs enriched in developing brains have recently been found to contain cryptic open-reading frames and are speculated…
View article: Author response: linc-mipep and linc-wrb encode micropeptides that regulate chromatin accessibility in vertebrate-specific neural cells
Author response: linc-mipep and linc-wrb encode micropeptides that regulate chromatin accessibility in vertebrate-specific neural cells Open
Full text Figures and data Side by side Abstract Editor's evaluation Introduction Results Discussion gMaterials and methods Appendix 1 Data availability References Decision letter Author response Article and author information Metrics Abst…
View article: Small-molecule ketone esters treat brain network abnormalities in an Alzheimer’s disease mouse model
Small-molecule ketone esters treat brain network abnormalities in an Alzheimer’s disease mouse model Open
Summary Altered brain network activity and the resulting hypersynchrony are important for the pathogenesis of cognitive decline in Alzheimer’s disease (AD) mouse models. Treatments that reduce epileptiform discharges (EDs) or network hyper…
View article: <i>linc-mipep</i> and <i>linc-wrb</i> encode micropeptides that regulate chromatin accessibility in vertebrate-specific neural cells
<i>linc-mipep</i> and <i>linc-wrb</i> encode micropeptides that regulate chromatin accessibility in vertebrate-specific neural cells Open
Thousands of long intergenic non-coding RNAs (lincRNAs) are transcribed throughout the vertebrate genome. A subset of lincRNAs enriched in developing brains has recently been found to contain cryptic open reading frames and are speculated …
View article: Prion protein gene mutation detection using long-read Nanopore sequencing
Prion protein gene mutation detection using long-read Nanopore sequencing Open
View article: Kroll, Dimitriadis, et al., 2022. Prion protein gene mutation detection using long-read Nanopore sequencing
Kroll, Dimitriadis, et al., 2022. Prion protein gene mutation detection using long-read Nanopore sequencing Open
Version uploaded 17/04/2022. Data for François Kroll*, Athanasios Dimitriadis*, Tracy Campbell, Lee Darwent, John Collinge, Simon Mead, Emmanuelle Viré. 2022. *co-first authors Prion protein gene mutation detectio…
View article: Kroll et al., 2022. Prion protein gene mutation detection using long-read Nanopore sequencing
Kroll et al., 2022. Prion protein gene mutation detection using long-read Nanopore sequencing Open
Version uploaded 09/04/2022. Data and code for Kroll, F. et al. 2022. Prion protein gene mutation detection using long-read Nanopore sequencing. https://doi.org/10.1101/2022.03.06.22271294 Present Zenodo archive contains heavier d…
View article: F0 knockout—single gene v3
F0 knockout—single gene v3 Open
Please cite 10.7554/eLife.59683 if you use this protocol. Note; our eLife publication used Version 2 of this protocol. Later version are subsequent improvements/simplifications. Get in touch for questions/suggestions twitter –@francois_kro…
View article: How to select the best gRNA(s) for frameshift knockouts in zebrafish v1
How to select the best gRNA(s) for frameshift knockouts in zebrafish v1 Open
Remember to cite the protocol if it is helpful to you! This protocol describes my approach (as of April 2022) to select gRNAs to generate frameshift knockouts in zebrafish using CRISPR-Cas9. It assumes you are selecting 3 gRNAs to generate…
View article: F0 knockout—single gene v2
F0 knockout—single gene v2 Open
Please cite 10.7554/eLife.59683 if you use this protocol. Note; our eLife publication used Version 2 of this protocol. Later version are subsequent improvements/simplifications. Get in touch for questions/suggestions twitter –@francois_kro…
View article: How to select the best gRNA(s) for frameshift knockouts in zebrafish v1
How to select the best gRNA(s) for frameshift knockouts in zebrafish v1 Open
Remember to cite the protocol if it is helpful to you! This protocol describes my approach (as of April 2022) to select gRNAs to generate frameshift knockouts in zebrafish using CRISPR-Cas9. It assumes you are selecting 3 gRNAs to generate…
View article: Prion protein gene mutation detection using long-read Nanopore sequencing
Prion protein gene mutation detection using long-read Nanopore sequencing Open
Prion diseases are fatal neurodegenerative conditions that affect humans and animals. Rapid and accurate sequencing of the prion gene PRNP is paramount to human prion disease diagnosis and for animal surveillance programmes. Current method…
View article: F0 knockout—single gene v2
F0 knockout—single gene v2 Open
Remember to cite the publication if you use this protocol! Get in touch for questions twitter –@francois_kroll email –[email protected]
View article: A simple and effective F0 knockout method for rapid screening of behaviour and other complex phenotypes
A simple and effective F0 knockout method for rapid screening of behaviour and other complex phenotypes Open
Hundreds of human genes are associated with neurological diseases, but translation into tractable biological mechanisms is lagging. Larval zebrafish are an attractive model to investigate genetic contributions to neurological diseases. How…
View article: Author response: A simple and effective F0 knockout method for rapid screening of behaviour and other complex phenotypes
Author response: A simple and effective F0 knockout method for rapid screening of behaviour and other complex phenotypes Open
View article: A simple and effective F0 knockout method for rapid screening of behaviour and other complex phenotypes – Preprint
A simple and effective F0 knockout method for rapid screening of behaviour and other complex phenotypes – Preprint Open
Data and code for preprint Kroll, F. et al. 2020. A simple and effective F0 knockout method for rapid screening of behaviour and other complex phenotypes. https://www.biorxiv.org/content/10.1101/2020.06.04.133462v3 You can also navigate th…
View article: Kroll et al., 2020. A simple and effective F0 knockout method for rapid screening of behaviour and other complex phenotypes
Kroll et al., 2020. A simple and effective F0 knockout method for rapid screening of behaviour and other complex phenotypes Open
Data and code for Kroll, F. et al. 2020. A simple and effective F0 knockout method for rapid screening of behaviour and other complex phenotypes. https://doi.org/10.1101/2020.06.04.133462 You can also navigate through the files at: https:/…