Frank Bürmann
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View article: Mechanism of DNA capture by the MukBEF SMC complex and its inhibition by a viral DNA mimic
Mechanism of DNA capture by the MukBEF SMC complex and its inhibition by a viral DNA mimic Open
Ring-like structural maintenance of chromosome (SMC) complexes are crucial for genome organization and operate through mechanisms of DNA entrapment and loop extrusion. Here, we explore the DNA loading process of the bacterial SMC complex M…
View article: Mechanism of DNA entrapment by the MukBEF SMC complex and its inhibition by a viral DNA mimic
Mechanism of DNA entrapment by the MukBEF SMC complex and its inhibition by a viral DNA mimic Open
Summary Ring-like structural maintenance of chromosomes (SMC) complexes are crucial for genome organization and operate through mechanisms of DNA entrapment and loop extrusion. Here, we explore the DNA loading process of the bacterial SMC …
View article: Cyclical MinD membrane affinity differences are not necessary for MinD gradient formation in Bacillus subtilis
Cyclical MinD membrane affinity differences are not necessary for MinD gradient formation in Bacillus subtilis Open
Proteins can diffuse micrometers in seconds, yet bacterial cells are able to maintain stable protein gradients. The best studied bacterial protein gradient is the Min system of Escherichia coli. In rod-shaped bacteria the MinCD proteins pr…
View article: Membrane affinity difference between MinD monomer and dimer is not crucial for MinD gradient formation in Bacillus subtilis
Membrane affinity difference between MinD monomer and dimer is not crucial for MinD gradient formation in Bacillus subtilis Open
Proteins can diffuse micrometers in seconds, yet bacterial cells are able to maintain stable protein gradients. The best studied bacterial protein gradient is the Min system of Escherichia coli. In rod-shaped bacteria the MinCD proteins pr…
View article: Membrane affinity difference between MinD monomer and dimer is not crucial for MinD gradient formation in Bacillus subtilis
Membrane affinity difference between MinD monomer and dimer is not crucial for MinD gradient formation in Bacillus subtilis Open
Proteins can diffuse micrometers in seconds, yet bacterial cells are able to maintain stable protein gradients. The best studied bacterial protein gradient is the Min system of Escherichia coli. In rod-shaped bacteria the MinCD proteins pr…
View article: The bacterial actin-like cell division protein FtsA forms curved antiparallel double filaments upon binding of FtsN
The bacterial actin-like cell division protein FtsA forms curved antiparallel double filaments upon binding of FtsN Open
Cell growth and division of walled bacteria depend on the synthesis and remodelling of peptidoglycan (PG). These activities are carried out by two multiprotein complexes, the elongasome and the divisome during cell elongation and division,…
View article: Cryo-EM structure of MukBEF reveals DNA loop entrapment at chromosomal unloading sites
Cryo-EM structure of MukBEF reveals DNA loop entrapment at chromosomal unloading sites Open
Summary The ring-like structural maintenance of chromosomes (SMC) complex MukBEF folds the genome of Escherichia coli and related bacteria into large loops, presumably by active DNA loop extrusion. MukBEF activity within the replication te…
View article: Phospho-regulation of the Shugoshin - Condensin interaction at the centromere in budding yeast
Phospho-regulation of the Shugoshin - Condensin interaction at the centromere in budding yeast Open
Correct bioriented attachment of sister chromatids to the mitotic spindle is essential for chromosome segregation. In budding yeast, the conserved protein shugoshin (Sgo1) contributes to biorientation by recruiting the protein phosphatase …
View article: Phospho-regulation of the Shugoshin - Condensin interaction at the centromere in budding yeast
Phospho-regulation of the Shugoshin - Condensin interaction at the centromere in budding yeast Open
Correct bioriented attachment of sister chromatids to mitotic spindle is essential for chromosome segregation. The conserved protein shugoshin (Sgo1) contributes in budding yeast to biorientation by recruiting the protein phosphatase PP2A-…
View article: A folded conformation of MukBEF and Cohesin
A folded conformation of MukBEF and Cohesin Open
Structural maintenance of chromosomes (SMC)-kleisin complexes organize chromosomal DNAs in all domains of life, where they have key roles in chromosome segregation, DNA repair and regulation of gene expression. They function through topolo…
View article: Structure of Full-Length SMC and Rearrangements Required for Chromosome Organization
Structure of Full-Length SMC and Rearrangements Required for Chromosome Organization Open
Multi-subunit SMC complexes control chromosome superstructure and promote chromosome disjunction, conceivably by actively translocating along DNA double helices. SMC subunits comprise an ABC ATPase "head" and a "hinge" dimerization domain …
View article: MOLECULAR-CELL-D-16-01779 Bürmann et al
MOLECULAR-CELL-D-16-01779 Bürmann et al Open
Original data of gels, Western blots and dilution spot assays as reported in Bürmann et al., 2017, Mol Cell
View article: Control of Smc Coiled Coil Architecture by the ATPase Heads Facilitates Targeting to Chromosomal ParB/parS and Release onto Flanking DNA
Control of Smc Coiled Coil Architecture by the ATPase Heads Facilitates Targeting to Chromosomal ParB/parS and Release onto Flanking DNA Open
Smc/ScpAB promotes chromosome segregation in prokaryotes, presumably by compacting and resolving nascent sister chromosomes. The underlying mechanisms, however, are poorly understood. Here, we investigate the role of the Smc ATPase activit…