Fraser P. McCready
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View article: Hypersynchronous iPSC-derived SHANK2 neuronal networks are rescued by mGluR5 agonism
Hypersynchronous iPSC-derived SHANK2 neuronal networks are rescued by mGluR5 agonism Open
Variants in the SHANK2 gene, linked to neurodevelopmental disorders like autism, were studied using human iPSC-derived neurons and multielectrode arrays. We compared two isogenic pairs of SHANK2 cell lines and found that SHANK2 networks ex…
View article: Disease-linked mutations dysregulate neuronal condensate physical properties, composition, and RNA translation
Disease-linked mutations dysregulate neuronal condensate physical properties, composition, and RNA translation Open
Local RNA translation is essential for development. In neurons, deficient local translation linked with mutations in scaffold proteins results in dysregulated dendrite and dendritic spine growth. However, mechanisms by which these proteins…
View article: Hypersynchronous iPSC-derived<i>SHANK2</i>neuronal networks are rescued by mGluR5 agonism
Hypersynchronous iPSC-derived<i>SHANK2</i>neuronal networks are rescued by mGluR5 agonism Open
Variants in the gene encoding the postsynaptic scaffolding protein SHANK2 are associated with several neurodevelopmental disorders, including autism spectrum disorder. Here, we used in vitro multielectrode arrays and pharmacological manipu…
View article: Calcium-Dependent Hyperexcitability in Human Stem Cell–Derived Rett Syndrome Neuronal Networks
Calcium-Dependent Hyperexcitability in Human Stem Cell–Derived Rett Syndrome Neuronal Networks Open
View article: Hyperexcitability in human<i>MECP2</i>null neuronal networks manifests as calcium-dependent reverberating super bursts
Hyperexcitability in human<i>MECP2</i>null neuronal networks manifests as calcium-dependent reverberating super bursts Open
Rett syndrome (RTT) patients show abnormal developmental trajectories including loss of language and repetitive hand movements but also have signs of cortical hyperexcitability such as seizures. RTT is predominantly caused by mutations in …
View article: Directed differentiation of human hindbrain neuroepithelial stem cells recapitulates cerebellar granule neurogenesis
Directed differentiation of human hindbrain neuroepithelial stem cells recapitulates cerebellar granule neurogenesis Open
Cerebellar granule neurons (CGNs) are the most abundant neurons in the human brain. Dysregulation of their development underlies movement disorders and medulloblastomas. It is suspected that these disorders arise in progenitor states of th…
View article: Directed differentiation of human hindbrain neuroepithelial stem cells recapitulates cerebellar granule neurogenesis
Directed differentiation of human hindbrain neuroepithelial stem cells recapitulates cerebellar granule neurogenesis Open
Cerebellar granule neurons (CGNs) are the most abundant neurons in the human brain and modulate cerebellar output to the motor cortex. Dysregulation of CGN development underlies movement disorders and medulloblastomas. It is suspected that…
View article: Multielectrode Arrays for Functional Phenotyping of Neurons from Induced Pluripotent Stem Cell Models of Neurodevelopmental Disorders
Multielectrode Arrays for Functional Phenotyping of Neurons from Induced Pluripotent Stem Cell Models of Neurodevelopmental Disorders Open
In vitro multielectrode array (MEA) systems are increasingly used as higher-throughput platforms for functional phenotyping studies of neurons in induced pluripotent stem cell (iPSC) disease models. While MEA systems generate large amounts…