Frederick Sachs
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View article: Hall Effect Sensor for Animal Geomagnetic Navigation
Hall Effect Sensor for Animal Geomagnetic Navigation Open
The magnetic compass has been a valuable human navigation tool for hundreds of years. Yet animals are known to align themselves with the earth’s magnetic field without using ferrimagnets.
View article: Adherent cell remodeling on micropatterns is modulated by Piezo1 channels
Adherent cell remodeling on micropatterns is modulated by Piezo1 channels Open
Adherent cells utilize local environmental cues to make decisions on their growth and movement. We have previously shown that HEK293 cells grown on the fibronectin stripe patterns were elongated. Here we show that Piezo1 function is involv…
View article: Functional analyses of heteromeric human PIEZO1 Channels
Functional analyses of heteromeric human PIEZO1 Channels Open
PIEZO1 and PIEZO2 are mechanosensitive channels (MSCs) important for cellular function and mutations in them lead to human disorders. We examined how functional heteromers form between subunits of PIEZO1 using the mutants E2117K, E2117D, a…
View article: Enantiomeric Aβ peptides inhibit the fluid shear stress response of PIEZO1
Enantiomeric Aβ peptides inhibit the fluid shear stress response of PIEZO1 Open
Traumatic brain injury (TBI) elevates Abeta (Aβ) peptides in the brain and cerebral spinal fluid. Aβ peptides are amphipathic molecules that can modulate membrane mechanics. Because the mechanosensitive cation channel PIEZO1 is gated by me…
View article: Heterogeneous Cytoskeletal Force Distribution Delineates the Onset Ca2+ Influx Under Fluid Shear Stress in Astrocytes
Heterogeneous Cytoskeletal Force Distribution Delineates the Onset Ca2+ Influx Under Fluid Shear Stress in Astrocytes Open
Mechanical perturbations increase intracellular Ca2+ in cells, but the coupling of mechanical forces to the Ca2+ influx is not well understood. We used a microfluidic chamber driven with a high-speed pressure servo to generate defined flui…
View article: Investigating the structural dynamics of the PIEZO1 channel activation and inactivation by coarse‐grained modeling
Investigating the structural dynamics of the PIEZO1 channel activation and inactivation by coarse‐grained modeling Open
The PIEZO channels, a family of mechanosensitive channels in vertebrates, feature a fast activation by mechanical stimuli (eg, membrane tension) followed by a slower inactivation. Although a medium‐resolution structure of the trimeric form…
View article: Unsupervised Idealization of Ion Channel Recordings by Minimum Description Length: Application to Human PIEZO1-Channels
Unsupervised Idealization of Ion Channel Recordings by Minimum Description Length: Application to Human PIEZO1-Channels Open
Researchers can investigate the mechanistic and molecular basis of many physiological phenomena in cells by analyzing the fundamental properties of single ion channels. These analyses entail recording single channel currents and measuring …
View article: Unsupervised idealization of ion channel recordings by Minimum Description Length: Application to human PIEZO1-channels
Unsupervised idealization of ion channel recordings by Minimum Description Length: Application to human PIEZO1-channels Open
Researchers can investigate the mechanistic and molecular basis of many physiological phenomena in cells by analyzing the fundamental properties of single ion channels. These analyses entail recording single channel currents and measuring …
View article: Mechanical stress activates NMDA receptors in the absence of agonists
Mechanical stress activates NMDA receptors in the absence of agonists Open
While studying the physiological response of primary rat astrocytes to fluid shear stress in a model of traumatic brain injury (TBI), we found that shear stress induced Ca 2+ entry. The influx was inhibited by MK-801, a specific pore block…
View article: Human PIEZO1 Ion Channel Functions as a Split Protein
Human PIEZO1 Ion Channel Functions as a Split Protein Open
PIEZO1 is a mechanosensitive eukaryotic cation-selective channel that rapidly inactivates in a voltage-dependent manner. We previously showed that a fluorescent protein could be encoded within the hPIEZO1 sequence without loss of function.…
View article: Adaptation Independent Modulation of Auditory Hair Cell Mechanotransduction Channel Open Probability Implicates a Role for the Lipid Bilayer
Adaptation Independent Modulation of Auditory Hair Cell Mechanotransduction Channel Open Probability Implicates a Role for the Lipid Bilayer Open
The auditory system is able to detect movement down to atomic dimensions. This sensitivity comes in part from mechanisms associated with gating of hair cell mechanoelectric transduction (MET) channels. MET channels, located at the tops of …
View article: Removal of the mechanoprotective influence of the cytoskeleton reveals PIEZO1 is gated by bilayer tension
Removal of the mechanoprotective influence of the cytoskeleton reveals PIEZO1 is gated by bilayer tension Open
Mechanosensitive ion channels are force-transducing enzymes that couple mechanical stimuli to ion flux. Understanding the gating mechanism of mechanosensitive channels is challenging because the stimulus seen by the channel reflects forces…