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View article: SpyCatcher-Multiplicity Tunes Nanoscaffold Hydrogels for Enhanced Catalysis of Regulated Enzymes
SpyCatcher-Multiplicity Tunes Nanoscaffold Hydrogels for Enhanced Catalysis of Regulated Enzymes Open
This study presents a strategy for enhancing hydrogel formation through SpyCatcher-mediated conjugation of nanoscale scaffold proteins. We demonstrate that SpyCatcher can facilitate hydrogel assembly with various nano-scaffolds of diverse …
View article: Key role of K+ and Ca2+ in high-yield ethanol production by S. Cerevisiae from concentrated sugarcane molasses
Key role of K+ and Ca2+ in high-yield ethanol production by S. Cerevisiae from concentrated sugarcane molasses Open
View article: Pseudomonas oryzisoli sp. nov., isolated from the soil of rice fields
Pseudomonas oryzisoli sp. nov., isolated from the soil of rice fields Open
A Gram-negative, rod-shaped, and aerobic bacterium Z8 T , was isolated from rice fields. It hydrolyses starch and urea, reduces nitrate, and is Tween-20, -40, and − 80, peroxidase and oxidase positive. It grows best at 35°C, 0.0–1.0% NaCl,…
View article: Ultrasound-Mediated DNA Transformation of Bacteria
Ultrasound-Mediated DNA Transformation of Bacteria Open
Ultrasound-mediated transformation has become a feasible means for plasmid transformation of microorganisms due to its simple operation, low influence from external factors, and low equipment requirements. This study investigated the effec…
View article: Ethanol Synthesis by <em>Saccharomyces cerevisiae</em>: Summary, Discovery, and Prospects
Ethanol Synthesis by <em>Saccharomyces cerevisiae</em>: Summary, Discovery, and Prospects Open
Saccharomyces cerevisiae (S. cerevisiae) ethanol fermentation has left an indelible trace in human history. It is regarded as the original beverage synthesis, and has developed into an essential energy supply for mankind today. However, th…
View article: A High-Throughput Screening Procedure (Py-Fe3+) for Enhancing Ethanol Production by Saccharomyces cerevisiae Using ARTP Random Mutagenesis
A High-Throughput Screening Procedure (Py-Fe3+) for Enhancing Ethanol Production by Saccharomyces cerevisiae Using ARTP Random Mutagenesis Open
Saccharomyces cerevisiae is an important microbial organization involved in ethanol synthesis. Mutant strains that can withstand multiple pressures during this process are critical to the industrial development of biofuels. In this study, …
View article: Enhancing the Production of Pinene in Escherichia coli by Using a Combination of Shotgun, Product-Tolerance and I-SceI Cleavage Systems
Enhancing the Production of Pinene in Escherichia coli by Using a Combination of Shotgun, Product-Tolerance and I-SceI Cleavage Systems Open
Tolerance breeding through genetic engineering, sequence and omics analyses, and gene identification processes are widely used to synthesize biofuels. The majority of related mechanisms have been shown to yield endogenous genes with high e…
View article: Cell-Free Escherichia coli Synthesis System Based on Crude Cell Extracts: Acquisition of Crude Extracts and Energy Regeneration
Cell-Free Escherichia coli Synthesis System Based on Crude Cell Extracts: Acquisition of Crude Extracts and Energy Regeneration Open
Cell-free synthetic biology is advancing with unprecedented control and design. The development of cell-free biosynthesis involves both pure enzyme and crude enzyme systems. The relatively cheap crude enzyme system is more suitable for the…
View article: Enhanced Production of Pterostilbene in Escherichia coli Through Directed Evolution and Host Strain Engineering
Enhanced Production of Pterostilbene in Escherichia coli Through Directed Evolution and Host Strain Engineering Open
Pterostilbene is a derivative of resveratrol with a higher bioavailability and biological activity, which shows antioxidant, anti-inflammatory, antitumor, and antiaging activities. Here, directed evolution and host strain engineering were …
View article: Recent Advances in Metabolically Engineered Microorganisms for the Production of Aromatic Chemicals Derived From Aromatic Amino Acids
Recent Advances in Metabolically Engineered Microorganisms for the Production of Aromatic Chemicals Derived From Aromatic Amino Acids Open
Aromatic compounds derived from aromatic amino acids are an important class of diverse chemicals with a wide range of industrial and commercial applications. They are currently produced via petrochemical processes, which are not sustainabl…
View article: Genomic and transcriptional changes in response to pinene tolerance and overproduction in evolved Escherichia coli
Genomic and transcriptional changes in response to pinene tolerance and overproduction in evolved Escherichia coli Open
α-Pinene is an important monoterpene, which is widely used as a flavoring agent and in fragrances, pharmaceuticals and biofuels. Although an evolved strain Escherichia coli YZFP, which had higher tolerance to pinene and titer, has b…
View article: Enhancing Production of Pinene in Escherichia coli by Using a Combination of Tolerance, Evolution, and Modular Co-culture Engineering
Enhancing Production of Pinene in Escherichia coli by Using a Combination of Tolerance, Evolution, and Modular Co-culture Engineering Open
α-Pinene is a natural and active monoterpene, which is widely used as a flavoring agent and in fragrances, pharmaceuticals, and biofuels. Although it has been successfully produced by genetically engineered microorganisms, the production l…
View article: Metabolic engineering for the microbial production of isoprenoids: Carotenoids and isoprenoid-based biofuels
Metabolic engineering for the microbial production of isoprenoids: Carotenoids and isoprenoid-based biofuels Open