Fufeng Liu
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View article: Application of multiple genomic-editing technologies in Streptomyces fungicidicus for improved enduracidin yield
Application of multiple genomic-editing technologies in Streptomyces fungicidicus for improved enduracidin yield Open
View article: A Molecular Logic Gate Enables Unconventional Super-resolution Same-Day Imaging of Lysosome Membrane in Live Cells
A Molecular Logic Gate Enables Unconventional Super-resolution Same-Day Imaging of Lysosome Membrane in Live Cells Open
Lysosomes are acidic membrane-bound organelles that aid digestion, excretion, and cell renewal. The lysosomal membranes are essential for maintaining lysosomal functions and cellular homeostasis. In this work, we developed a molecular "NOR…
View article: A microbiome war in our gut
A microbiome war in our gut Open
View article: The Microbiome Protocols eBook initiative: Building a bridge to microbiome research
The Microbiome Protocols eBook initiative: Building a bridge to microbiome research Open
The Microbiome Protocols eBook (MPB) serves as a crucial bridge, filling gaps in microbiome protocols for both wet experiments and data analysis. The first edition, launched in 2020, featured 152 meticulously curated protocols, garnering w…
View article: Rational Design of a Yeast-derived 3’,5’-bisphosphate Nucleotidase with Improved Substrate Specificity
Rational Design of a Yeast-derived 3’,5’-bisphosphate Nucleotidase with Improved Substrate Specificity Open
In recent years, a convenient phosphatase-coupled sulfotransferase assay method has been proven to be applicable to most sulfotransferases. The central principle of the method is that phosphatase specifically degrades 3'-phosphoadenosine-5…
View article: IPAD-DB: a manually curated database for experimentally verified inhibitors of proteins associated with Alzheimer’s disease
IPAD-DB: a manually curated database for experimentally verified inhibitors of proteins associated with Alzheimer’s disease Open
Alzheimer’s disease (AD) is a universal neurodegenerative disease with the feature of progressive dementia. Currently, there are only seven Food and Drug Administration-approved drugs for the treatment of AD, which merely offer temporary r…
View article: Lyophilization process optimization and molecular dynamics simulation of mRNA-LNPs for SARS-CoV-2 vaccine
Lyophilization process optimization and molecular dynamics simulation of mRNA-LNPs for SARS-CoV-2 vaccine Open
View article: Heterologous expression and fibrillary characterization of the microtubule-binding domain of tau associated with tauopathies
Heterologous expression and fibrillary characterization of the microtubule-binding domain of tau associated with tauopathies Open
Background Neurofibrillary tangles (NFTs) are one of the most common pathological characteristics of Alzheimer's disease (AD). The NFTs are mainly composed of hyperphosphorylated microtubule-associated tau. Thus, recombinant tau is urgentl…
View article: Identification of Binding Sites in the Nucleotide-Binding Domain of P-Glycoprotein for a Potent and Nontoxic Modulator, the Amine-Containing Monomeric Flavonoid <b>FM04</b>
Identification of Binding Sites in the Nucleotide-Binding Domain of P-Glycoprotein for a Potent and Nontoxic Modulator, the Amine-Containing Monomeric Flavonoid <b>FM04</b> Open
We have previously discovered an amine-containing flavonoid monomer FM04 as a potent P-glycoprotein (P-gp) inhibitor (EC50 = 83 nM). Here, a series of photoactive FM04 analogues were synthesized and used together w…
View article: Discrimination of psychrophilic enzymes using machine learning algorithms with amino acid composition descriptor
Discrimination of psychrophilic enzymes using machine learning algorithms with amino acid composition descriptor Open
Introduction Psychrophilic enzymes are a class of macromolecules with high catalytic activity at low temperatures. Cold-active enzymes possessing eco-friendly and cost-effective properties, are of huge potential application in detergent, t…
View article: Brazilin-7-Acetate, a Novel Potential Drug of Parkinson's Disease, Hinders the Formation of Α-Synuclein Fibril, Mitigates Cytotoxicity, and Decreases Oxidative Stress
Brazilin-7-Acetate, a Novel Potential Drug of Parkinson's Disease, Hinders the Formation of Α-Synuclein Fibril, Mitigates Cytotoxicity, and Decreases Oxidative Stress Open
View article: Heterologous expression and characterization of an M4 family extracellular metalloprotease for detergent application
Heterologous expression and characterization of an M4 family extracellular metalloprotease for detergent application Open
Proteolytic enzymes stand out as the most widely employed category utilized in manufacturing industry. A new protease was separated from Planococcus sp.11815 strain and named as nprS-15615 in this research. The gene of this protease has no…
View article: Bionics design of affinity peptide inhibitors for SARS-CoV-2 RBD to block SARS-CoV-2 RBD-ACE2 interactions
Bionics design of affinity peptide inhibitors for SARS-CoV-2 RBD to block SARS-CoV-2 RBD-ACE2 interactions Open
View article: Asymmetric reduction of conjugated C C bonds by immobilized fusion of old yellow enzyme and glucose dehydrogenase
Asymmetric reduction of conjugated C C bonds by immobilized fusion of old yellow enzyme and glucose dehydrogenase Open
Asymmetric reduction of the conjugated CC bonds by the old yellow enzymes (OYEs) presents a promising field in the synthesis of chiral chemicals. Nevertheless, few natural OYEs have been applied in large-scale applications due to the requ…
View article: SPSED: A Signal Peptide Secretion Efficiency Database
SPSED: A Signal Peptide Secretion Efficiency Database Open
DATA REPORT article Front. Bioeng. Biotechnol., 18 January 2022Sec. Industrial Biotechnology Volume 9 - 2021 | https://doi.org/10.3389/fbioe.2021.819789
View article: Asymmetric Reduction of Conjugated C=C Bonds by Immobilized Fusion of Old Yellow Enzyme and Glucose Dehydrogenase
Asymmetric Reduction of Conjugated C=C Bonds by Immobilized Fusion of Old Yellow Enzyme and Glucose Dehydrogenase Open
View article: Aromadendrin: a dual amyloid promoter to accelerate fibrillization and reduce cytotoxicity of both amyloid-β and hIAPP
Aromadendrin: a dual amyloid promoter to accelerate fibrillization and reduce cytotoxicity of both amyloid-β and hIAPP Open
Aromadendrin, as a protective amyloid promoter, is discovered to simultaneously promote the aggregation of Aβ and hIAPP peptides and reduce the amyloid-induced cytotoxicity.
View article: Rational design of a Yarrowia lipolytica derived lipase for improved thermostability
Rational design of a Yarrowia lipolytica derived lipase for improved thermostability Open
View article: Factors Influencing Recombinant Protein Secretion Efficiency in Gram-Positive Bacteria: Signal Peptide and Beyond
Factors Influencing Recombinant Protein Secretion Efficiency in Gram-Positive Bacteria: Signal Peptide and Beyond Open
Signal peptides are short peptides directing newly synthesized proteins toward the secretory pathway. These N-terminal signal sequences are ubiquitous to all prokaryotes and eukaryotes. Signal peptides play a significant role in recombinan…
View article: Highly efficient soluble expression, purification and characterization of recombinant Aβ42 from<i>Escherichia coli</i>
Highly efficient soluble expression, purification and characterization of recombinant Aβ42 from<i>Escherichia coli</i> Open
A novel high-yield expression and purification method for Aβ42 based on a fusion with maltose binding protein followed by the soluble polypeptide linker (NANP)3and a modified tobacco etch virus cleavage site before the Aβ42 was …
View article: 5-Hydroxycyclopenicillone Inhibits β-Amyloid Oligomerization and Produces Anti-β-Amyloid Neuroprotective Effects In Vitro
5-Hydroxycyclopenicillone Inhibits β-Amyloid Oligomerization and Produces Anti-β-Amyloid Neuroprotective Effects In Vitro Open
The oligomer of β-amyloid (Aβ) is considered the main neurotoxin in Alzheimer’s disease (AD). Therefore, the inhibition of the formation of Aβ oligomer could be a target for AD therapy. In this study, with the help of the dot blotting assa…
View article: Brazilin inhibits fibrillogenesis of human islet amyloid polypeptide, disassembles mature fibrils, and alleviates cytotoxicity
Brazilin inhibits fibrillogenesis of human islet amyloid polypeptide, disassembles mature fibrils, and alleviates cytotoxicity Open
Inhibitory effect of brazilin on the fibrillogenesis of hIAPP was explored using biochemical, biophysical, cytobiological and molecular simulation experiments. Brazilin was a potential compound for therapeutic treatment of type II diabetes…
View article: Brazilin inhibits amyloid β-protein fibrillogenesis, remodels amyloid fibrils and reduces amyloid cytotoxicity
Brazilin inhibits amyloid β-protein fibrillogenesis, remodels amyloid fibrils and reduces amyloid cytotoxicity Open
Soluble amyloid β-protein (Aβ) oligomers, the main neurotoxic species, are predominantly formed from monomers through a fibril-catalyzed secondary nucleation. Herein, we virtually screened an in-house library of natural compounds and disco…