Fuguo Jiang
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View article: The RNA splicing factor PRPF8 is required for left-right organiser cilia differentiation and determination of cardiac left-right asymmetry via regulation of Arl13b splicing
The RNA splicing factor PRPF8 is required for left-right organiser cilia differentiation and determination of cardiac left-right asymmetry via regulation of Arl13b splicing Open
Cilia function in the left-right organizer (LRO) is critical for determining internal organ asymmetry in vertebrates. To further understand the genetics of left-right asymmetry, we isolated a mouse mutant with laterality defects, l11Jus27,…
View article: [Expressions of miR-27a-3p mRNA and <i>YAP1</i> mRNA in Breast Cancer and the Relationship With Clinicopathology and Survival Prognosis].
[Expressions of miR-27a-3p mRNA and <i>YAP1</i> mRNA in Breast Cancer and the Relationship With Clinicopathology and Survival Prognosis]. Open
A significant downregulation of miR-27a-3p mRNA and upregulation of YAP1 mRNA are observed in breast tumor tissues. The low expression of miR-27a-3p mRNA and the high expression of YAP1 mRNA are associated with adverse clinicopathological …
View article: MicroRNA‑20a promotes non‑small cell lung cancer proliferation by upregulating <i>PD‑L1</i> by targeting <i>PTEN</i>
MicroRNA‑20a promotes non‑small cell lung cancer proliferation by upregulating <i>PD‑L1</i> by targeting <i>PTEN</i> Open
Non-small cell lung cancer (NSCLC) remains one of the most common malignant tumors worldwide. The aim of the present study was to investigate the possibility of microRNA-20a (miR-20a) as a biomarker and therapeutic target for the diagnosis…
View article: Ceftazidime-Avibactam Resistance in Klebsiella pneumoniae Sequence Type 11 Due to a Mutation in Plasmid-Borne blakpc-2 to blakpc-33, in Henan, China
Ceftazidime-Avibactam Resistance in Klebsiella pneumoniae Sequence Type 11 Due to a Mutation in Plasmid-Borne blakpc-2 to blakpc-33, in Henan, China Open
This is the first report from Henan to show that treatment with CZA for 6 days can cause mutations and change the phenotype from CZA sensitive to resistant. Therefore, routine testing for drug susceptibility and carbapenemase phenotypes sh…
View article: Temperature-responsive competitive inhibition of CRISPR-Cas9
Temperature-responsive competitive inhibition of CRISPR-Cas9 Open
SUMMARY CRISPR–Cas immune systems utilize RNA-guided nucleases to protect bacteria from bacteriophage infection. Bacteriophages have in turn evolved inhibitory ‘anti-CRISPR’ (Acr) proteins, including six inhibitors (AcrIIA1-6) that can blo…
View article: Extension of the crRNA enhances Cpf1 gene editing in vitro and in vivo
Extension of the crRNA enhances Cpf1 gene editing in vitro and in vivo Open
Engineering of the Cpf1 crRNA has the potential to enhance its gene editing efficiency and non-viral delivery to cells. Here, we demonstrate that extending the length of its crRNA at the 5′ end can enhance the gene editing efficiency of Cp…
View article: Disabling Cas9 by an anti-CRISPR DNA mimic
Disabling Cas9 by an anti-CRISPR DNA mimic Open
Natural inhibitors of Cas9 pretend to be DNA and block target binding, and using them in human cells can reduce off-target events.
View article: Disabling Cas9 by an anti-CRISPR DNA mimic
Disabling Cas9 by an anti-CRISPR DNA mimic Open
CRISPR-Cas9 gene editing technology is derived from a microbial adaptive immune system, where bacteriophages are often the intended target. Natural inhibitors of CRISPR-Cas9 enable phages to evade immunity and show promise in controlling C…
View article: Regulation of Retinoic Acid Inducible Gene-I (RIG-I) Activation by the Histone Deacetylase 6
Regulation of Retinoic Acid Inducible Gene-I (RIG-I) Activation by the Histone Deacetylase 6 Open
Retinoic acid inducible gene-I (RIG-I) is a cytosolic pathogen recognition receptor that initiates the immune response against many RNA viruses. Upon RNA ligand binding, RIG-I undergoes a conformational change facilitating its homo-oligome…
View article: Nucleosome breathing and remodeling constrain CRISPR-Cas9 function
Nucleosome breathing and remodeling constrain CRISPR-Cas9 function Open
The CRISPR-Cas9 bacterial surveillance system has become a versatile tool for genome editing and gene regulation in eukaryotic cells, yet how CRISPR-Cas9 contends with the barriers presented by eukaryotic chromatin is poorly understood. He…
View article: Author response: Nucleosome breathing and remodeling constrain CRISPR-Cas9 function
Author response: Nucleosome breathing and remodeling constrain CRISPR-Cas9 function Open
Article Figures and data Abstract eLife digest Introduction Results Discussion Materials and methods References Decision letter Author response Article and author information Metrics Abstract The CRISPR-Cas9 bacterial surveillance system h…
View article: Structural basis for m7G recognition and 2′-O-methyl discrimination in capped RNAs by the innate immune receptor RIG-I
Structural basis for m7G recognition and 2′-O-methyl discrimination in capped RNAs by the innate immune receptor RIG-I Open
Significance The cytosolic innate immune receptor Retinoic Acid Inducible Gene-I (RIG-I) is the principal detector of pathogenic RNAs carrying a 5′-triphosphate (5′ppp). Self RNAs like mRNAs evade recognition by RIG-I due to posttranscript…
View article: The autoinhibitory CARD2-Hel2i Interface of RIG-I governs RNA selection
The autoinhibitory CARD2-Hel2i Interface of RIG-I governs RNA selection Open
RIG-I (Retinoic Acid Inducible Gene-I) is a cytosolic innate immune receptor that detects atypical features in viral RNAs as foreign to initiate a Type I interferon signaling response. RIG-I is present in an autoinhibited state in the cyto…