Fuliang Du
YOU?
Author Swipe
View article: Cerebrovascular compromise and cognitive decline driven by chronic heart failure
Cerebrovascular compromise and cognitive decline driven by chronic heart failure Open
Objective This study investigated the effects of chronic heart failure with decreased LVEF on small cerebral vascular disease (CSVD) and associated cognitive impairment. Methods This study encompassed a cohort of 73 patients diagnosed with…
View article: Interaction and regulatory expression of Polycomb and NuRD complexes in mouse embryonic stem cell under PKC inhibition
Interaction and regulatory expression of Polycomb and NuRD complexes in mouse embryonic stem cell under PKC inhibition Open
Polycomb repressive complexes (PRC) and nucleosome remodeling and deacetylase (NuRD) complex are crucial for regulating the expression of pluripotent and developmental genes and maintaining the characteristics of mouse embryonic stem cells…
View article: H3K9me3 and H3K27me3 are epigenetic barriers to somatic cell nuclear transfer in rabbits
H3K9me3 and H3K27me3 are epigenetic barriers to somatic cell nuclear transfer in rabbits Open
Somatic cell nuclear transfer (NT) is associated with aberrant changes in epigenetic reprogramming that disrupt embryo development. Here, we characterized the epigenetic patterns of histone 3 lysine 9 trimethylation (H3K9me3) and histone 3…
View article: Regulation of PKCi-mediated pluripotency and gene expression by polycomb complex 1 in mouse embryonic stem cells
Regulation of PKCi-mediated pluripotency and gene expression by polycomb complex 1 in mouse embryonic stem cells Open
These results indicate that PKCi activates both the canonical and non-canonical PRC1 pathways, finely regulates the expression of mES pluripotent and developmental genes, and helps maintain mES in a poised state between self-renewal and li…
View article: Overcoming the <scp>H4K20me3</scp> epigenetic barrier improves somatic cell nuclear transfer reprogramming efficiency in mice
Overcoming the <span>H4K20me3</span> epigenetic barrier improves somatic cell nuclear transfer reprogramming efficiency in mice Open
Epigenetic reprogramming during fertilization and somatic cell nuclear transfer (NT) is required for cell plasticity and competent development. Here, we characterize the epigenetic modification pattern of H4K20me3, a repressive histone sig…
View article: Nucleosome remodeling and deacetylation complex and MBD3 influence mouse embryonic stem cell naïve pluripotency under inhibition of protein kinase C
Nucleosome remodeling and deacetylation complex and MBD3 influence mouse embryonic stem cell naïve pluripotency under inhibition of protein kinase C Open
The pluripotency of naïve mouse embryonic stem cells (mES) is regulated by multiple signaling pathways, with inhibition of protein kinase C (PKCi) playing a particularly important role in maintaining naïve mES. However, the regulatory func…
View article: Epigenetic dynamics of H4K20me3 modification during oocyte maturation and early reprogramming of somatic cell nuclear transfer goat embryos.
Epigenetic dynamics of H4K20me3 modification during oocyte maturation and early reprogramming of somatic cell nuclear transfer goat embryos. Open
These results indicate that NT embryos exhibit insufficient H4K20me3 modification compared with IVF and PA embryos during early reprogramming, suggesting the existence of a resistant memory of differentiated cell nuclear architecture. Thes…
View article: PKC inhibitors regulate stem cell self-renewal by regulating H3K27me3 and H3K9me3.
PKC inhibitors regulate stem cell self-renewal by regulating H3K27me3 and H3K9me3. Open
Embryonic stem cell (ESC) research is critical to the scientific community, as their application in regenerative medicine can be widely beneficial. ESCs eventually withdraw from their self-renewal program and subsequently differentiate int…
View article: Efficient mutagenesis targeting the <i>IFNAR1</i> gene in mice using a combination of Cas9 protein and dual gRNAs.
Efficient mutagenesis targeting the <i>IFNAR1</i> gene in mice using a combination of Cas9 protein and dual gRNAs. Open
We injected mouse zygotes with combinations of Cas9 protein, Cas9 mRNA, and two gRNAs targeting a single exon of type I interferon receptor (IFNAR1) to determine the gene targeting efficiencies. Cas9 protein produced on-targe…
View article: Methyl-CpG-binding domain 3 (Mbd3) is an important regulator for apoptosis in mouse embryonic stem cells.
Methyl-CpG-binding domain 3 (Mbd3) is an important regulator for apoptosis in mouse embryonic stem cells. Open
Methyl-CpG-binding domain 3 (Mbd3) is a core repressor complex component. Although Mbd3 is required for the pluripotency of embryonic stem cells (ES), the role of Mbd3 in mouse ES (mES) cell apoptosis remains undefined. In this study naïve…
View article: Magnesium is a critical element for competent development of bovine embryos
Magnesium is a critical element for competent development of bovine embryos Open
View article: Nuclear reprogramming by somatic cell nuclear transfer – the cattle story
Nuclear reprogramming by somatic cell nuclear transfer – the cattle story Open
Somatic cell nuclear transfer (cloning) returns a differentiated cell to a totipotent status; a process termed nuclear reprogramming. Nuclear transfer has potential applications in agriculture and biomedicine, but is limited by low efficie…
View article: Deriving rabbit embryonic stem cells by small molecule inhibitors.
Deriving rabbit embryonic stem cells by small molecule inhibitors. Open
We previously developed pluripotent rabbit embryonic stem cells (rbES) using a culture system supplemented with basic fibroblast growth factor (bFGF) and leukemia inhibitory factor (LIF), noggin and Y-27632 (referred to as iFLY). In presen…
View article: Dynamic patterns of H3K4me3, H3K27me3, and Nanog during rabbit embryo development.
Dynamic patterns of H3K4me3, H3K27me3, and Nanog during rabbit embryo development. Open
Epigenetic modification and expression of key pluripotent factors are critical for development, cell fate determination, and differentiation in early embryos. In this study, we systematically examined the dynamic patterns of histone modifi…
View article: ROCK inhibitor Y-27632 maintains the propagation and characteristics of hair follicle stem cells.
ROCK inhibitor Y-27632 maintains the propagation and characteristics of hair follicle stem cells. Open
Hair follicle stem cells (HFSCs) are an important source for skin tissue engineering studies and clinical applications. Here, we describe a differential enrichment approach to derive HFSCs from hair follicles of vibrissae and ear skin usin…
View article: Efficient generation of FVII gene knockout mice using CRISPR/Cas9 nuclease and truncated guided RNAs
Efficient generation of FVII gene knockout mice using CRISPR/Cas9 nuclease and truncated guided RNAs Open
We investigated the effects of 5′-end truncated CRISPR RNA-guided Cas9 nuclease (tru-RGN, 17/18 nucleotides) on genome editing capability in NIH/3T3 cells, and its efficiencies on generating Factor VII ( FVII ) gene-knockout (KO) mice. In …
View article: Derivation of Rabbit Embryonic Stem Cells from Vitrified–Thawed Embryos
Derivation of Rabbit Embryonic Stem Cells from Vitrified–Thawed Embryos Open
The rabbit is a useful animal model for regenerative medicine. We previously developed pluripotent rabbit embryonic stem cell (rbESC) lines using fresh embryos. We also successfully cryopreserved rabbit embryos by vitrification. In the pre…