Fumihiro Azuma
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View article: Selection of Cord Blood Unit by CD34+ Cell and GM-CFU Numbers and Allele-Level HLA Matching in Single Cord Blood Transplantation
Selection of Cord Blood Unit by CD34+ Cell and GM-CFU Numbers and Allele-Level HLA Matching in Single Cord Blood Transplantation Open
In Japan, only single-unit cord blood transplantations (CBTs) are typically performed, and their number has increased over the last 23 years, with ongoing improvement in results. In most cases, CBTs with multiple HLA mismatches are used, o…
View article: Adverse effect of donor-specific anti-human leukocyte antigen (HLA) antibodies directed at HLA-DP/-DQ on engraftment in cord blood transplantation
Adverse effect of donor-specific anti-human leukocyte antigen (HLA) antibodies directed at HLA-DP/-DQ on engraftment in cord blood transplantation Open
View article: EVALUATION OF AN AUTOMATED COLONY-COUNTING SYSTEM AS A STANDARD COLONY-FORMING ASSAY METHOD FOR CORD BLOOD BANKING
EVALUATION OF AN AUTOMATED COLONY-COUNTING SYSTEM AS A STANDARD COLONY-FORMING ASSAY METHOD FOR CORD BLOOD BANKING Open
コロニー形成試験（CFU試験）は，移植後の造血回復能を予測できる臍帯血品質試験法として用いられているが，コロニーの種類と数を顕微鏡下にて目視で判定することから主観的要素を完全に排除することが難しく，判定者ごとに結果の不揃いが生じやすい．これを解決するために，CFU試験における自動コロニー判定システム（STEMvisionTM）の有用性について30例の臍帯血を対象に検討した．まず，樹脂製擬似コロニーを埋め込んだ精度管理用プレートでシステムの安定的稼働を確認した．同システムでの…
View article: HLA class I allele–lacking leukocytes predict rare clonal evolution to MDS/AML in patients with acquired aplastic anemia
HLA class I allele–lacking leukocytes predict rare clonal evolution to MDS/AML in patients with acquired aplastic anemia Open
View article: Overview of HLA DNA typing technology
Overview of HLA DNA typing technology Open
HLA（Human Leukocyte Antigen＝ヒト白血球抗原）はその豊富な多型性からヒトゲノム多様性の研究対象となるだけでなく，自己非自己認識の根幹としての機能から，免疫学的研究でも重要な位置を占める。さらにドナーと患者の適合性は移植や一部の血小板輸血において大きな影響を与えるため，適合度を評価するためのHLAタイピングは臨床上も重要な技術である。かつてタイピングは血清学的手法で行われてきたが，分子生物学的理解が進んだ現在はHLA遺伝子の塩基配列から遺伝子型（アレ…
View article: A frequent nonsense mutation in exon 1 across certain HLA-A and -B alleles in leukocytes of patients with acquired aplastic anemia
A frequent nonsense mutation in exon 1 across certain HLA-A and -B alleles in leukocytes of patients with acquired aplastic anemia Open
Leukocytes that lack HLA allelic expression are frequently detected in patients with acquired aplastic anemia (AA) who respond to immunosuppressive therapy (IST), although the exact mechanisms underlying the HLA loss and HLA allele reperto…
View article: PS1110 THE IDENTIFICATION OF A COMMON LOSS‐OF‐FUNCTION MUTATION ACROSS DIFFERENT HLA CLASS ALLELES FREQUENTLY DETECTED IN PATIENTS WITH ACQUIRED APLASTIC ANEMIA
PS1110 THE IDENTIFICATION OF A COMMON LOSS‐OF‐FUNCTION MUTATION ACROSS DIFFERENT HLA CLASS ALLELES FREQUENTLY DETECTED IN PATIENTS WITH ACQUIRED APLASTIC ANEMIA Open
Background: The presence of HLA class I allele‐lacking leukocytes due to the copy‐number neutral loss of heterozygosity in the short arm of chromosome 6 (6pLOH) or HLA allelic mutations is compelling evidence supporting the notion that cyt…
View article: THE PREVALENCE OF <i>JK*A</i> with c.130A SINGLE NUCLEOTIDE POLYMORPHISM IN THE JAPANESE BLOOD DONORS AND EXPRESSION ANALYSIS OF Jk<sup>a</sup> AND Jk3 ANTIGENS ON THEIR RED BLOOD CELLS
THE PREVALENCE OF <i>JK*A</i> with c.130A SINGLE NUCLEOTIDE POLYMORPHISM IN THE JAPANESE BLOOD DONORS AND EXPRESSION ANALYSIS OF Jk<sup>a</sup> AND Jk3 ANTIGENS ON THEIR RED BLOOD CELLS Open
抗Jka，抗Jkbは遅発性溶血性副作用の原因となるが，クロスマッチでは検出困難である場合が多く問題となっている．最近，JK*A遺伝子（またはJK*01）の一塩基置換c.130G＞A（アリル名JK*01W.01）に伴うアミノ酸置換（p.Glu44Lys）によるJkaおよびJk3抗原の減少が報告された．本研究では日本人献血者におけるJK遺伝子c.130Aの頻度，さらにJkaとJk3抗原の発現への影響を検討した．日本人献血者2,017名でのJK遺伝子c.130Aの頻度は38.5%…
View article: Risk of HLA Homozygous Cord Blood Transplantation: Implications for Induced Pluripotent Stem Cell Banking and Transplantation
Risk of HLA Homozygous Cord Blood Transplantation: Implications for Induced Pluripotent Stem Cell Banking and Transplantation Open
Clinical application of induced pluripotent stem cells (iPS) in autologous settings has just begun. To overcome the high time and cost barriers in the individual production of autologous iPS, the use of allogeneic iPS with a homozygous hum…
View article: Evolutionary basis of HLA-DPB1 alleles affects acute GVHD in unrelated donor stem cell transplantation
Evolutionary basis of HLA-DPB1 alleles affects acute GVHD in unrelated donor stem cell transplantation Open
Key Points HLA-DPB1 alleles diverged into 2 major groups according to highly conserved DNA sequences Ex3-3′UTR. Two evolutionarily different HLA-DPB1 gene regions complementarily affect aGVHD in HLA-DPB1 mismatch UR-HCT.
View article: GENOTYPING OF RED CELL BLOOD GROUPS BY THE LIQUID BEAD ARRAY SYSTEM (LUMINEX)
GENOTYPING OF RED CELL BLOOD GROUPS BY THE LIQUID BEAD ARRAY SYSTEM (LUMINEX) Open
DNAによる赤血球型遺伝子タイピングは血清学的検査を補う有用な方法である．しかし，人種による多型性の相違もあるため，日本人を対象とした赤血球型遺伝子検査法の開発および検討を行った．
View article: HLA-DPB1 Mismatch Induces Low Hematological Relapse and Beneficial Survival in Single Unit Cord Blood Transplantation
HLA-DPB1 Mismatch Induces Low Hematological Relapse and Beneficial Survival in Single Unit Cord Blood Transplantation Open
The risks of HLA-A,-B,-C,-DRB1,-DQB1 and -DPB1 allele disparity for transplant-related events in cord blood transplantation (CBT) have not been fully analyzed. We conducted cooperative research to elucidate the impact of each HLA locus in …
View article: High Risk HLA Allele for Severe Acute Graft-Versus-Host-Disease and Mortality in Unrelated Donor Hematopoietic Stem Cell Transplantation
High Risk HLA Allele for Severe Acute Graft-Versus-Host-Disease and Mortality in Unrelated Donor Hematopoietic Stem Cell Transplantation Open
HLA molecules play an important role for immunoreactivity in allogeneic hematopoietic stem cell transplantation. To elucidate the effect of specific HLA alleles on acute graft-versus-host disease (aGVHD), we conducted a retrospective analy…
View article: High-risk HLA alleles for severe acute graft- <i>versus</i> -host disease and mortality in unrelated donor bone marrow transplantation
High-risk HLA alleles for severe acute graft- <i>versus</i> -host disease and mortality in unrelated donor bone marrow transplantation Open
HLA molecules play an important role for immunoreactivity in allogeneic hematopoietic stem cell transplantation. To elucidate the effect of specific HLA alleles on acute graft-versus-host disease, we conducted a retrospective analysis usin…
View article: Genome-wide surveillance of mismatched alleles for graft-versus-host disease in stem cell transplantation
Genome-wide surveillance of mismatched alleles for graft-versus-host disease in stem cell transplantation Open
Key Points GWAS can identify allele mismatch associated with aGVHD development. Three novel candidate loci for minor histocompatibility antigens significantly associate with aGVHD.
View article: Cost-efficient multiplex PCR for routine genotyping of up to nine classical HLA loci in a single analytical run of multiple samples by next generation sequencing
Cost-efficient multiplex PCR for routine genotyping of up to nine classical HLA loci in a single analytical run of multiple samples by next generation sequencing Open